Resistance to Freezing and Frozen Storage of Streptococcus thermophilus Is Related to Membrane Fatty Acid Composition

Béal, Catherine; Fonseca, Fernanda; Corrieu, Georges

doi:10.3168/jds.s0022-0302(01)74683-8

Cited by 114 publications

(86 citation statements)

References 33 publications

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“…bulgaricus CFL1 after freeze thawing in MRS broth was reported [8]. This difference in cell viability between strains was linked to the intrinsic resistance of the strain, the initial concentration, growth conditions and possibly the elimination of a large part of the sensitive population during the freezing step [9]. The strains were sensitive to a variation in the incubation temperature as shown in Table 4.…”

Section: Resultsmentioning

confidence: 99%

Assessment of the Potential of Lactic Acid Bacteria as Dried Starter Culture for Cereal Fermentation

Soro-Yao¹,

Aka²,

Thonart³

et al. 2014

TOBIOTJ

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and 0.61 h to 1.33 h, respectively. We observed that the strains were sensitive to a change in water activity (a w 0.32 final), storage and incubation temperatures. The Strain LF obtained a higher cell concentration and viability, as well as a lower g compared to those obtained by the other strains. The kinetic growth parameters (µ m , g) together with viability after stress treatments could be used for the screening of dried lactic acid starter cultures.

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Section: Resultsmentioning

confidence: 99%

Assessment of the Potential of Lactic Acid Bacteria as Dried Starter Culture for Cereal Fermentation

Soro-Yao¹,

Aka²,

Thonart³

et al. 2014

TOBIOTJ

View full text Add to dashboard Cite

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“…delbrueckii subsp. bulgaricus [8] [29] [47], Streptococcus thermophilus [48]. Loss of the viability of freeze-dried cultures is a consequence of cell damage at several target sites, namely the cell wall, the cell membrane and the DNA, as well as a result of membrane lipid oxidation [49].…”

Section: Discussionmentioning

confidence: 99%

Influence of Freezing and Freeze Drying on Intracellular Enzymatic Activity and Autolytic Properties of Some Lactic Acid Bacterial Strains

Kandil¹,

Soda²

2015

AiM

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Lactic acid bacteria possess several interesting properties of great economic importance. Improvement and stabilization of these industrially important features are an active research area at the present time. The objectives of this work are to study the effect of freezing and freeze-drying on the survival rate, autolytic activity and intracellular enzymatic activity of the main species of lactic acid bacteria used in the dairy industry. The article focused on several characteristics that were not well covered in the past. The obtained results revealed that both preservation methods have a significant effect on viability, autolytic activity and intracellular enzymatic activity. After six months of storage we found that frozen cultures exhibited higher survival rate, higher rate of intracellular enzymatic activity and lower rate of autolysis. The impact of conservation treatments was only strain specific in the case of survival rate. The results obtained lead to the selection of the best preservation method for the selected cultures based on survival rate, autolytic activity and intracellular enzymatic activity.

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“…Although genes associated with migration, quiescence, or hibernation strategies have been studied for a variety of metazoans, microorganisms, particularly bacteria in temperate continental climate zones, have received considerably less attention. However, it is known that cryotolerance in some species is associated with fatty acid changes in membrane lipids (3,8), as well as certain culture conditions (11,21,35). Studies of polar psychrophiles have revealed adaptations such as lipid modification to maintain membrane fluidity, accumulation of polyols, genome adaptations, and production of cold shock proteins and cold-active enzymes, including enzymes important for protein synthesis (4,9,19,24,26).…”

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confidence: 99%

Freeze-Thaw Tolerance and Clues to the Winter Survival of a Soil Community

Walker

Palmer

Voordouw

2006

Appl Environ Microbiol

160

115

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Although efforts have been made to sample microorganisms from polar regions and to investigate a few of the properties that facilitate survival at freezing or subzero temperatures, soil communities that overwinter in areas exposed to alternate freezing and thawing caused by Foehn or Chinook winds have been largely overlooked. We designed and constructed a cryocycler to automatically subject soil cultures to alternating freeze-thaw cycles. After 48 freeze-thaw cycles, control Escherichia coli and Pseudomonas chlororaphis isolates were no longer viable. Mixed cultures derived from soil samples collected from a Chinook zone showed that the population complexity and viability were reduced after 48 cycles. However, when bacteria that were still viable after the freeze-thaw treatments were used to obtain selected cultures, these cultures proved to be >1,000-fold more freeze-thaw tolerant than the original consortium. Single-colony isolates obtained from survivors after an additional 48 freeze-thaw cycles were putatively identified by 16S RNA gene fragment sequencing. Five different genera were recognized, and one of the cultures, Chryseobacterium sp. strain C14, inhibited ice recrystallization, a property characteristic of antifreeze proteins that prevents the growth of large, potentially damaging ice crystals at temperatures close to the melting temperature. This strain was also notable since cell-free medium derived from cultures of it appeared to enhance the multiple freeze-thaw survival of another isolate, Enterococcus sp. strain C8. The results of this study and the development of a cryocycler should allow further investigations into the biochemical and soil community adaptations to the rigors of a Chinook environment.

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Resistance to Freezing and Frozen Storage of Streptococcus thermophilus Is Related to Membrane Fatty Acid Composition

Cited by 114 publications

References 33 publications

Assessment of the Potential of Lactic Acid Bacteria as Dried Starter Culture for Cereal Fermentation

Assessment of the Potential of Lactic Acid Bacteria as Dried Starter Culture for Cereal Fermentation

Influence of Freezing and Freeze Drying on Intracellular Enzymatic Activity and Autolytic Properties of Some Lactic Acid Bacterial Strains

Freeze-Thaw Tolerance and Clues to the Winter Survival of a Soil Community

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