Resistance to fatigue of single muscle fibres from Xenopus related to succinate dehydrogenase and myofibrillar ATPase activities

Laarse, Willem J. van der; Lännergren, Jan; Pc, Diegenbach

doi:10.1113/expphysiol.1991.sp003526

Cited by 34 publications

(28 citation statements)

References 11 publications

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“…This association, which confirms similar results reported previously for single muscle cells (30), demonstrates that fatigue profiles from a single cell can be used to predict oxidative capacity (29). Thus, for the purposes of the present investigation, fatigability profiles in response to the incremental frequency protocol can be considered analogous to the intrinsic oxidative capacity of the cell.…”

Section: Discussionsupporting

confidence: 76%

Relationship between intracellular Po₂ recovery kinetics and fatigability in isolated single frog myocytes

Kindig¹,

Walsh²,

Howlett³

et al. 2005

Journal of Applied Physiology

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. Relationship between intracellular PO2 recovery kinetics and fatigability in isolated single frog myocytes. J Appl Physiol 98: 2316 -2319, 2005. First published February 3, 2005 doi:10.1152/japplphysiol.00355.2004.-In single frog skeletal myocytes, a linear relationship exists between "fatigability" and oxidative capacity. The purpose of this investigation was to study the relationship between the intracellular PO2 (PIO 2 ) offset kinetics and fatigability in single Xenopus laevis myocytes to test the hypothesis that PIO 2 offset kinetics would be related linearly with myocyte fatigability and, by inference, oxidative capacity. Individual myocytes (n ϭ 30) isolated from lumbrical muscle were subjected to a 2-min bout of isometric peak tetanic contractions at either 0.25-or 0.33-Hz frequency while PIO 2 was measured continuously via phosphorescence quenching techniques. The mean response time (MRT; time to 63% of the overall response) for PIO 2 recovery from contracting values to resting baseline was calculated. After the initial square-wave constantfrequency contraction trial, each cell performed an incremental contraction protocol [i.e., frequency increase every 2 min from 0.167, 0.25, 0.33, 0.5, 1.0, and 2.0 Hz until peak tension fell below 50% of initial values (TTF)]. TTF values ranged from 3.39 to 10.04 min for the myocytes. The PIO 2 recovery MRT ranged from 26 to 146 s. A significant (P Ͻ 0.05), negative relationship (MRT ϭ Ϫ12.68TTF ϩ 168.3, r 2 ϭ 0.605) between TTF and PIO 2 recovery MRT existed. These data demonstrate a significant correlation between fatigability and oxidative phosphorylation recovery kinetics consistent with the notion that oxidative capacity determines, in part, the speed with which skeletal muscle can recover energetically to alterations in metabolic demand. muscle fiber; fatigue; metabolic control; oxidative capacity AN INVERSE, PROPORTIONAL RELATIONSHIP has been proposed to exist between the speed of phosphocreatine (PCr) resynthesis and muscle mitochondrial capacity (18,22). Previous investigations in both animals (23) and humans (e.g., Refs. 14, 20, 21) have confirmed this postulation. Specifically, Paganini and colleagues (23) demonstrated a linear relationship between PCr recovery times after electrically stimulated contractions and citrate synthase activity in rat skeletal muscle.O 2 uptake (V O 2 ) onset kinetics are considered dependent, at least in part, on maximal aerobic capacity. Indeed, aerobic exercise training that induces significant increases in muscle oxidative capacity also speeds V O 2 kinetics (4, 8, 10). However, this can occur before any discernible increase in maximal V O 2 and muscle oxidative capacity (24). To date, no rigorous investigation studying the relationship between maximal aerobic capacity and V O 2 off-kinetics exists. However, given that PCr and V O 2 kinetics appear to be reasonably well matched (1,19,26), it is possible that a strong relationship exists between maximal aerobic capacity and V O 2 off-kinetics.The purpose of the present inv...

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Section: Discussionsupporting

confidence: 76%

Relationship between intracellular Po₂ recovery kinetics and fatigability in isolated single frog myocytes

Kindig¹,

Walsh²,

Howlett³

et al. 2005

Journal of Applied Physiology

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“…The relationship between aerobic-oxidative energy supply (SDH) and energy demand of contraction, as reflected by actomyosin myofibrillar ATPase activity (qATPase), may be more indicative of fatigue resistance than mitochondrial content. 27 Because of these findings and because the average fiber SDH:qATPase ratio did not differ between the TA and QF muscles, Gregory et al hypothesized that fatigue would be comparable between muscles. Our results strongly support this hypothesis because the TA and QF muscles each showed about 55% force loss over 180 CFTs.…”

mentioning

confidence: 99%

Fatigability and Variable-Frequency Train Stimulation of Human Skeletal Muscles

et al. 2003

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Background and Purpose. The quadriceps femoris (QF) and tibialis anterior (TA) muscles are often activated through the use of electrical stimulation by physical therapists. These 2 muscles are fundamentally different in regard to their fiber-type composition. Whether protocols developed using a given muscle can be applied to another muscle has seldom been questioned, even if they differ in fiber type. The purpose of this study was to test the hypothesis that torque augmentation during variable-frequency train (VFT) stimulation as compared with constantfrequency train (CFT) stimulation in the fatigued state would not differ between these muscles, even though the TA muscle has 50% relatively more slow fibers than the QF muscle relative to each muscle's overall composition. Subjects. Ten recreationally active men with no history of lower-extremity pathology participated in the study (mean ageϭ25 years, SDϭ4, rangeϭ19 -31; mean heightϭ179 cm, SDϭ5, rangeϭ170 -188; mean body massϭ80 kg, SDϭ15, rangeϭ63-111). Methods. The subjects' TA and QF muscles were stimulated with CFTs (six 200-microsecond square waves separated by 70 milliseconds) or VFTs (first interpulse intervalϭ5 milliseconds) that evoked an isometric contraction. Results. After potentiation, the torque-time integral and peak torque were not different for the VFT and CFT stimulation. Rise time was longer for the TA muscle than for the QF muscle and for CFT stimulation versus VFT stimulation (both approximately 40%). After 180 CFTs (50% duty cycle), peak torque decreased 56% overall, with no differences between muscles. Enhancement of the torque-time integral (25%) by VFT stimulation was not different between fatigued QF and TA muscles. Torque augmentation was due to the VFT stimulation evoking 27% greater peak torque and less slowing of rise time than the CFT stimulation (15% versus 30%). Discussion and Conclusion. The results indicate that the QF muscle may not necessarily fatigue more than the TA muscle. The results suggest that VFTs augment the force of fatigued, human skeletal muscle irrespective of fiber type. [Bickel CS, Slade JM, Warren GL, Dudley GA. Fatigability and variable-frequency train stimulation of human skeletal muscles. Phys Ther. 2003;83:366 -373.] S urface electrical stimulation is often applied to human skeletal muscles that differ in fiber-type composition. For example, the tibialis anterior (TA) muscle is sometimes stimulated in an effort to control foot drop. 1 However, we have observed that the quadriceps femoris (QF) muscle is probably the muscle most often activated with electrical stimulation during rehabilitation, thereby serving as the test bed for protocol development. Whether protocols developed using a given muscle can be applied to another muscle has seldom been questioned, despite the fact that the characteristics of individual fibers contribute to a muscle's ability to develop and maintain force. 2 The observation that, on average, the TA muscle has 50% relatively more slow fibers than the QF muscle relative to each ...

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“…CLFS increased fatigue resistance and this increase was unaffected by salbutamol, Fig 8. The effect of CLFS and salbutamol on the distribution of fiber types may account for these findings. Muscle fatigue can be considered the result of an imbalance between energy supply and energy demand [20,21]. Energy supply can be estimated by succinic dehydrogenase (SDH) activity and energy demand by myofibrillar ATPase activity.…”

Section: Figmentioning

confidence: 99%

Salbutamol and the Conditioning of Latissimus Dorsi for Cardiomyoplasty

Wright

Zhang

McClain

et al. 1999

Journal of Surgical Research

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Resistance to fatigue of single muscle fibres from Xenopus related to succinate dehydrogenase and myofibrillar ATPase activities

Cited by 34 publications

References 11 publications

Relationship between intracellular Po₂ recovery kinetics and fatigability in isolated single frog myocytes

Relationship between intracellular Po₂ recovery kinetics and fatigability in isolated single frog myocytes

Fatigability and Variable-Frequency Train Stimulation of Human Skeletal Muscles

Salbutamol and the Conditioning of Latissimus Dorsi for Cardiomyoplasty

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Resistance to fatigue of single muscle fibres from Xenopus related to succinate dehydrogenase and myofibrillar ATPase activities

Cited by 34 publications

References 11 publications

Relationship between intracellular Po2 recovery kinetics and fatigability in isolated single frog myocytes

Relationship between intracellular Po2 recovery kinetics and fatigability in isolated single frog myocytes

Fatigability and Variable-Frequency Train Stimulation of Human Skeletal Muscles

Salbutamol and the Conditioning of Latissimus Dorsi for Cardiomyoplasty

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Relationship between intracellular Po₂ recovery kinetics and fatigability in isolated single frog myocytes

Relationship between intracellular Po₂ recovery kinetics and fatigability in isolated single frog myocytes