Resistance to desiccation and skin fatty acids in outbreak strains of methicillin-resistant Staphylococcus aureus

Farrington, M.; Brenwald, N P; Haines, David; Walpole, Enid

doi:10.1099/00222615-36-1-56

Cited by 57 publications

(33 citation statements)

References 3 publications

(3 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Antimicrobial activity of fatty acids was stated to be dependent on chain length and unsaturation degree [7]. Longchain unsaturated fatty acids exhibit inhibitory activity against many bacteria even including methicillin-resistant S. aureus [8]. For instance, oleic and linoleic acids were reported as potent antibacterial [9].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Donkey Milk against Dermatomycotic Fungi and Foodborne Bacteria

Koutb¹,

Khider²,

Ali³

et al. 2016

IJBMR

View full text Add to dashboard Cite

Abstract:The antimicrobial activity of donkey milk was examined against 3 bacterial and 3 fungal strains selected on the basis of their relevance as human pathogens. All samples of donkey milk exhibited antimicrobial activity against dermatomycotic fungi and foodborne pathogen bacteria. The highest antimicrobial activity was recorded against Trichophyton mentagrophytes and T. rubrum with minimal lethal concentration of 32 mg/ml. In respect to foodborne pathogenic bacteria, Gram-positive bacteria (Bacillus cereus and Staphylococcus aureus) were more sensitive to donkey milk than Gram-negative bacteria (E. coli) with minimal lethal concentrations of 32, 64 and 128 mg/ml respectively. Donkey milk kept about 60 to 62% of its antimicrobial activity against B. cereus and S. aurous respectively, after digestion with pepsin (2 mg/ml), suggesting that the fatty acids of donkey milk has the highest antimicrobial effect. While the antimicrobial activity against dermatomycotic fungi and Gram-negative bacteria not affected after digestion with pepsin. To explain the antifungal capability of donkey milk against dermatomycotic fungi, fatty acids were analyzed by gas chromatography. Fatty acids analysis indicated that the major constituents in donkey milk lipid are oleic (25.4%), palmitic (23.75%), linolenic (20.04%), arachidic (3.58%) and stearic (3.26%), which have antimicrobial activity. Finally, on the basis of results obtained in the current study, the antimicrobial activity of donkey milk against Staphylococcus aureus and dermatomycotic fungi, specially T. mentagrophytes and T. rubrum which frequently cause acute or chronic inflammatory tinea corporis; may be considered as a valuable natural product with novel functional protection properties in cosmetics and pharmaceutical industries.

show abstract

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Donkey Milk against Dermatomycotic Fungi and Foodborne Bacteria

Koutb¹,

Khider²,

Ali³

et al. 2016

IJBMR

View full text Add to dashboard Cite

show abstract

“…Oleic acid is a bactericidal agent against important pathogenic microorganisms including methicillin-resistant Staphylococcus aureus and Helicobacter pylori (Farrington et al 1992). Oleic acid has been moderately docked with Ala41 residue of RRF as compared to other fatty acids of AH (Fig.…”

Section: Resultsmentioning

confidence: 99%

Computational approaches to the in vitro antibacterial activity of Allium hirtifolium Boiss against gentamicin-resistant Escherichia coli: focus on ribosome recycling factor

Ali

Chehri

Karimi

et al. 2017

In Silico Pharmacol.

View full text Add to dashboard Cite

Persian shallot, Allium hirtifolium Boiss. (AH), is an Iranian native medicinal plant belongs to Alliaceae family. Here, we investigated in vitro antibacterial activity of hydro-alcoholic extract derived from bulbs of AH. We also employed in silico molecular docking to decipher mechanisms of its antibacterial effects. Minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) against E. coli ATCC 25922 were determined. Molecular docking was performed for major phytochemicals of AH against ribosome recycling factor (RRF). E. coli ATCC 25922 was gentamicin-resistant while AH showed MIC (42 ± 18 lg/ml) and MBC (106 ± 36 lg/ml) against E. coli. In silico results reported all phytochemicals of AH shown acceptable negative binding affinity (kcal/-mol) with RRF.

show abstract

“…S. aureus survival within the hospital environment is well documented [11][12][13], and some epidemic strains can remain viable for longer on cotton lint than other strains [14]. The mechanisms of enhanced survival are poorly understood, but resistance to desiccation [13,15], adherence [16], and the ability to survive on human skin [15][16][17] have been implicated.…”

Section: Neonatal Units Provide Ideal Environments Formentioning

confidence: 99%

“…To test the hypothesis, we compared in itro survival rates of 20 S. aureus strains, including 5 SA5385, on Stomahesive2 using a modified desiccation method [15]. Overnight cultures of each strain grown in 10 ml Brain Heart Infusion broth (Oxoid) were centrifuged, washed, and re-suspended in 4 ml phosphate buffered saline (PBS).…”

Section: Resistance To Desiccationmentioning

confidence: 99%

A five year outbreak of methicillin-susceptible Staphylococcus aureus phage type 53,85 in a regional neonatal unit

et al. 2000

View full text Add to dashboard Cite

Article ReuseUnless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. SUMMARYWe identified a 5-year outbreak of a methicillin-susceptible Staphylococcus aureus (MSSA) strain, affecting 202 babies on a neonatal unit, by routine weekly phage typing all S. aureus isolates. Multiple staged control measures including strict emphasis on hand hygiene, environmental and staff surveillance sampling, and application of topical hexachlorophane powder failed to end the outbreak. S. aureus PT 53,85 (SA5385) was found on opened packs of Stomahesive2, used as a neonatal skin protectant. Only following the implementation of aseptic handling of Stomahesive, and the use of topical mupirocin for staff nasal carriers of SA5385, and for babies colonized or infected with S. aureus, did the isolation rate of SA5385 decline. DNA fingerprinting indicated that 95 % of SA5385 isolates were clonal. In itro death rates of SA5385 on Stomahesive with human serum were significantly lower than on Stomahesive alone (P l 0n04), and on cotton sheet with serum (P l 0n04), highlighting the potential of this material as a survival niche. Phage typing remains a valuable, inexpensive and simple method for monitoring nosocomial MSSA infection.

show abstract

Resistance to desiccation and skin fatty acids in outbreak strains of methicillin-resistant Staphylococcus aureus

Cited by 57 publications

References 3 publications

Antimicrobial Activity of Donkey Milk against Dermatomycotic Fungi and Foodborne Bacteria

Antimicrobial Activity of Donkey Milk against Dermatomycotic Fungi and Foodborne Bacteria

Computational approaches to the in vitro antibacterial activity of Allium hirtifolium Boiss against gentamicin-resistant Escherichia coli: focus on ribosome recycling factor

A five year outbreak of methicillin-susceptible Staphylococcus aureus phage type 53,85 in a regional neonatal unit

Contact Info

Product

Resources

About