Resistance to Anthracnose (Colletotrichum lentis, Race 0) in Lens spp. Germplasm

Abstract

Anthracnose, caused by the fungal pathogen Colletotrichum lentis, is a severe disease of lentil (Lens culinaris) causing premature defoliation, necrotic stem lesions that lead to plant wilting and death in susceptible varieties. Two races of C. lentis (0 and 1) have been described so far. Race 0 is the most virulent one and limited genetic resistance is available to date. To address this scarcity of resistance, we screened a germplasm collection covering different Lens spp. originating from different countries for C. lentis race 0 resistance. Leaf and stem damage and plant mortality were assessed on seedlings inoculated under controlled conditions. A significant variability was observed among accession. Most of the collection studied was highly susceptible, but some levels of resistance were identified in about 15% of the accessions. The highest levels of resistance were identified in L. ervoides accessions PI572330, PI572334 and PI572338. Moderate resistance was also identified in 10 L. culinaris ssp. culinaris accessions but not in the remaining species studied. Selected accessions showed potential to integrate several breeding programs.

Status of Phytotoxins Isolated from Necrotrophic Fungi Causing Diseases on Grain Legumes

“…Biological control is also an alternative method, which uses various antagonistic microorganisms such as Bacillus subtilis [ 110 ]. In legumes, genetic sources of resistance have been developed in common bean, soybean, and lentil [ 111 , 112 , 113 ], where some varieties with partial resistance to the disease have been identified.…”

Conventional and new-breeding technologies for improving disease resistance in lentil (Lens culinaris Medik)

“…The following paragraphs briefly explain some of the commonly used screening methods for various diseases. Masoor-93, ILL 4605, ILL 857, ILL5590, ILL 5593, ILL 5244, ILL 5725, ILL 179, ILL 195, ILL 201, ILL 5698, ILL 5700, ILL 5883, ILL 6212, ILL 2439, ILL 5562, Indianhead, 96, 507, 712, 859, 112082, 123452, 123514 and123801 Nguyen et al, 2001;Ye et al, 2002;Tivoli et al, 2006;Sari et al, 2018;Bedasa, 2021 Lentil rust IPL81, PL639, L4147, L4149, DPL 15, LL147, L4076, Pant Lentil 4, LH82-6, NDL92-1, Gudo (a resistant cultivar), R 186 Buchwaldt et al, 2004;Buchwaldt et al, 2018;Barilli et al (2020) Resistant to Blight, rust and Viral disease 66013-6 Hussain et al, 2008 Resistant to Stemphylium blight…”

“…Gene pyramiding can be used to accumulate such multiple disease resistance in a single genotype through marker-assisted selection. Barilli et al (2020) proposed using moderate to highly resistant germplasm of diverse origin as donors for Aschochyta blight resistance to broaden the genetic diversity of the evolved resistant genotypes. Interspecific hybridization among newly identified resistant lentil species is very important to develop pre-breeding materials for disease resistance breeding.…”

Analysis of Crop Genetic and Germplasm Diversity

“…In legumes, studies on resistance to anthracnose in lentils (Lens spp.) [29], the inheritance and expressivity of neoplasm trait in crosses between the domestic pea (Pisum sativum subsp. sativum) and wild pea (Pisum sativum subsp.…”

“…Most of the collection studied was highly susceptible, but some levels of resistance were identified in about 15% of the accessions. Selected accessions showed potential to integrate several breeding programs [29]. The Neoplasm trait in pea pods is reported to be due to the lack of ultraviolet (UV) light in glasshouse conditions or in response to pea weevil (Bruchus pisorum L.) damage.…”