Resistance selection, mechanism and stability of Spodoptera litura (Lepidoptera: Noctuidae) to methoxyfenozide

Rehan, Adeel; Freed, Shoaib

doi:10.1016/j.pestbp.2014.02.001

Cited by 38 publications

(16 citation statements)

References 28 publications

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“…DBHs are not toxic to mammals (LD 50 > 5000 mg/kg by ingestion for methoxyfenozide, which compares favorably to the LD 50 = 430 mg/kg by ingestion of the pyrethroid permethrin commonly used on LLINs) and would therefore be ideally suitable for bed net-based strategies, where low toxicity is essential. Moreover, although resistance to DBH has been experimentally induced in larvae from a number of insect agricultural pests and has been described in wild populations with varying reports on the possible biochemical and genetic basis [24, 49–51], it has rapidly reversed when the intervention was withdrawn [24, 50]. Although resistance to DBH or any other active compound will inevitably occur at some point, different strategies such as rotation, mosaics, or combination with insecticide could be utilized to delay its emergence.…”

Section: Discussionmentioning

confidence: 99%

Disrupting Mosquito Reproduction and Parasite Development for Malaria Control

et al. 2016

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The control of mosquito populations with insecticide treated bed nets and indoor residual sprays remains the cornerstone of malaria reduction and elimination programs. In light of widespread insecticide resistance in mosquitoes, however, alternative strategies for reducing transmission by the mosquito vector are urgently needed, including the identification of safe compounds that affect vectorial capacity via mechanisms that differ from fast-acting insecticides. Here, we show that compounds targeting steroid hormone signaling disrupt multiple biological processes that are key to the ability of mosquitoes to transmit malaria. When an agonist of the steroid hormone 20-hydroxyecdysone (20E) is applied to Anopheles gambiae females, which are the dominant malaria mosquito vector in Sub Saharan Africa, it substantially shortens lifespan, prevents insemination and egg production, and significantly blocks Plasmodium falciparum development, three components that are crucial to malaria transmission. Modeling the impact of these effects on Anopheles population dynamics and Plasmodium transmission predicts that disrupting steroid hormone signaling using 20E agonists would affect malaria transmission to a similar extent as insecticides. Manipulating 20E pathways therefore provides a powerful new approach to tackle malaria transmission by the mosquito vector, particularly in areas affected by the spread of insecticide resistance.

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Section: Discussionmentioning

confidence: 99%

Disrupting Mosquito Reproduction and Parasite Development for Malaria Control

et al. 2016

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“…PBO is an insecticide synergist known to inhibit the activity of cytochrome P450 enzymes (Willoughby et al, 2007). Previous studies have suggested that resistance against chlorpyrifos, β-cypermethrin and methomyl in S. litura and Helicoverpa armigera is monooxygenase-mediated (Sasabe et al, 2004;Bues et al, 2005;Ahmad et al, 2007;Ahmad et al, 2009;Rehan & Freed, 2014;Dawkar et al, 2015). The toxicity of chlorpyrifos, β-cypermethrin and methomyl against S. litura increased 3.25-, 5.73-and 3.12-fold, respectively, when applied with PBO, as compared with chlorpyrifos, β-cypermethrin and methomyl alone (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Identification of a novel cytochrome P450 CYP321B1 gene from tobacco cutworm (Spodoptera litura) and RNA interference to evaluate its role in commonly used insecticides

et al. 2016

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Insect cytochrome P450 monooxygenases (CYPs or P450s) play an important role in detoxifying insecticides leading to resistance in insect populations. A polyphagous pest, Spodoptera litura, has developed resistance to a wide range of insecticides. In the present study, a novel P450 gene, CYP321B1, was cloned from S. litura. The function of CYP321B1 was assessed using RNA interference (RNAi) and monitoring resistance levels for three commonly used insecticides, including chlorpyrifos, β-cypermethrin and methomyl. The full-length complementary DNA sequence of CYP321B1 is 1814 bp long with an open reading frame of 1 488 bp encoding 495 amino acid residues. Quantitative reverse-transcriptase polymerase chain reaction analyses during larval and pupal development indicated that CYP321B1 expression was highest in the midgut of fifth-instar larvae, followed by fat body and cuticle. The expression of CYP321B1 in the midgut was up-regulated by chlorpyrifos, β-cypermethrin and methomyl with both lethal concentration at 15% (LC ) (50, 100 and 150 μg/mL, respectively) and 50%(LC ) dosages (100, 200 and 300 μg/mL, respectively). Addition of piperonyl butoxide (PBO) significantly increased the toxicity of chlorpyrifos, β-cypermethrin and methomyl to S. litura, suggesting a marked synergism of the three insecticides with PBO and P450-mediated detoxification. RNAi-mediated silencing of CYP321B1 further increased mortality by 25.6% and 38.9% when the fifth-instar larvae were exposed to chlorpyrifos and β-cypermethrin, respectively, at the LC dose levels. The results demonstrate that CYP321B1 might play an important role in chlorpyrifos and β-cypermethrin detoxification in S. litura.

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“…2007a,b, 2008; Shad et al . ; Rehan & Shoaib 2014a,b). The development of resistance in insect pests can be delayed due to fitness cost of resistance (Carriere & Tabashnik ).…”

Section: Introductionmentioning

confidence: 99%

Lethal and sub‐lethal effects of spinosad on the life‐history traits of army worm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae), and its fitness cost of resistance

Rehan

Freed

2015

Entomological Research

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Spodoptera litura is one of the most destructive polyphagous insect pests, with more than 120 host‐plant species. In our present study, a field‐collected population of S. litura when selected with spinosad for 11 consecutive generations resulted in the development of 3921‐fold resistance to spinosad as compared to the susceptible strain. The spinosad‐resistant strain of S. litura had a relatively high fitness cost (0.17) as compared to the susceptible strain. Furthermore, the lethal and sub‐lethal effects of different concentrations of spinosad were checked on the susceptible strain at different levels; i.e., LC40, LC30, LC20 and LC10, which revealed that the impact of spinosad on the life‐history traits of S. litura increased with the increase in concentration of spinosad. A significant impact of spinosad was recorded on the larval duration, pre‐pupal weight, pupal duration, pupal weight, reproductive potential and adult emergence. The outcomes of the current research clearly indicate that fitness cost of spinosad and its sub‐lethal effects have a significant impact on population dynamics of S. litura, for which it can be incorporated in integrated pest management.

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Resistance selection, mechanism and stability of Spodoptera litura (Lepidoptera: Noctuidae) to methoxyfenozide

Cited by 38 publications

References 28 publications

Disrupting Mosquito Reproduction and Parasite Development for Malaria Control

Disrupting Mosquito Reproduction and Parasite Development for Malaria Control

Identification of a novel cytochrome P450 CYP321B1 gene from tobacco cutworm (Spodoptera litura) and RNA interference to evaluate its role in commonly used insecticides

Lethal and sub‐lethal effects of spinosad on the life‐history traits of army worm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae), and its fitness cost of resistance

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