Resistance response enhancement and reduction of Botrytis cinerea infection in strawberry fruit by Morchella conica mycelial extract

Vanti, Gulamnabi L.; Leshem, Y.; Masaphy, Segula

doi:10.1016/j.postharvbio.2021.111470

Cited by 19 publications

(13 citation statements)

References 48 publications

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“…are known [ 34 , 35 , 36 ]. For example, a Morchella conica mycelial extract led to upregulation of genes related to defense against biotic stress in the treated fruit [ 37 ]. It is already known that plants that are infected with pathogens after exposure to biological control agents may present high levels of catalase, peroxidase, polyphenol oxidases, superoxide dismutase and phenylalanine ammonia-lyase enzymes [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Mechanism of Action of Serratia rubidaea Mar61-01 against Botrytis cinerea in Strawberries

Alijani

Amini

Karimi

et al. 2022

Plants

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Several bacterial strains belonging to Serratia spp. possess biocontrol capability, both against phytopathogens and human pathogenic species, thanks to the production of secondary metabolites, including as a red-pink, non-diffusible pigment, 2-methyl-3-pentyl-6-methoxyprodiginine (prodigiosin). Botrytis cinerea is the causal agent of gray mold, which is an economically relevant disease of many crops worldwide. Gray mold is normally controlled by chemical fungicides, but the environmental and health concerns about the overuse of pesticides call for environmentally friendly approaches, such as the use of biocontrol agents. In this study, the efficacy of a specific strain of Serratia rubidaea (Mar61-01) and its metabolite prodigiosin were assessed against B. cinerea under in vitro and in vivo conditions. This strain was effective against B. cinerea, and the effect of prodigiosin was confirmed under in vitro and in vivo conditions. The strain suppressed mycelial growth of B. cinerea (71.72%) in the dual-culture method. The volatile compounds produced by the strain inhibited mycelial growth and conidia germination of B. cinerea by 65.01% and 71.63%, respectively. Efficacy of prodigiosin produced by S. rubidaea Mar61-01 on mycelial biomass of B. cinerea was 94.15% at the highest concentration tested (420 µg/mL). The effect of prodigiosin on plant enzymes associated with induction of resistance was also studied, indicating that the activity of polyphenol oxidase (PPO), superoxide dismutase (SOD) and phenylalanine ammonia lyase (PAL) were increased when prodigiosin was added to the B. cinerea inoculum on strawberry fruits, while catalase (CAT) and peroxidase (POD) did not change. In addition, the volatile organic compounds (VOCs) produced by S. rubidaea Mar61-01 reduced mycelial growth and inhibited conidial germination of B. cinerea in vitro. The findings confirmed the relevant role of prodigiosin produced by S. rubidaea Mar61-01 in the biocontrol of B. cinerea of strawberries, but also indicate that there are multiple mechanisms of action, where the VOCs produced by the bacterium and the plant-defense reaction may contribute to the control of the phytopathogen. Serratia rubidaea Mar61-01 could be a suitable strain, both to enlarge our knowledge about the potential of Serratia as a biocontrol agent of B. cinerea and to develop new biofungicides to protect strawberries in post-harvest biocontrol.

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Section: Discussionmentioning

confidence: 99%

Characterization of the Mechanism of Action of Serratia rubidaea Mar61-01 against Botrytis cinerea in Strawberries

Alijani

Amini

Karimi

et al. 2022

Plants

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“…Because of these limitations, there is an urgent demand to develop alternative approaches to reduce or even replace chemical fungicides for postharvest storage of fruits and vegetables. Promising approaches, including natural antimicrobials like chitosan [ 9 ], natamycin [ 10 ], and tea oil [ 11 ], GRAS (generally regarded as safe) substances like trisodium phosphate [ 12 ], and biological control agents [ 13 , 14 ], and elicitors [ 15 ] have been explored for the storage of postharvest fruits.…”

Section: Introductionmentioning

confidence: 99%

Postharvest quality maintenance of wax apple and guava fruits by use of a fermented broth of an ε-poly-l-lysine-producing Streptomyces strain

Bai

Zhang

et al. 2022

PLoS ONE

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ε-Poly-l-lysine (ε-PL) is a natural antimicrobial polymer with significant inhibitory activity against a broad spectrum of microorganisms, and nowadays used widely as a preservative in the food industry. In the present study, ε-PL broth was obtained from Streptomyces ahygroscopicus GIM8 fermentation in a nutrient-limited liquid medium. The in vitro antifungal activity of the broth against fruit pathogens Penicillium expansum and Colletotrichum gloeosporioides was investigated, and its usage for postharvest storage of two highly perishable fruits wax apple and guava was evaluated. Results showed that ε-PL concentration in the broth reached 0.61 g/L, and the nutrition level of the broth was low. The antifungal activity of ε-PL broth was comparable to that of the aqueous solution of ε-PL under the same concentration. Immersion with the diluted broth (200 mg/L ε-PL) markedly delayed the decline in the quality of postharvest wax apple and guava fruits during storage, and the decay incidences were also greatly decreased as compared to their respective controls (distilled water immersion). A further investigation demonstrated that the ε-PL broth immersion induced an increase in the activity of defense-related enzymes peroxidase and polyphenol oxidase in the two fruits during storage. The present study proved that the fermentation broth of ε-PL could be used as a promising alternative to high purity ε-PL and synthetic fungicides for preserving fruits at postharvest stage.

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“…However, several fungal pathogens attack strawberries due to their tender and juicy characteristics, among which Botrytis cinerea is the primary fungal pathogen, causing fruit decay that has resulted in severe economic losses to the strawberry industry [2]. B. cinerea is a necrotrophic fungus that attacks the fruits in the field or after harvest and causes gray mold in more than 200 crop species [3]. The fungal conidia are highly abundant and ubiquitous and generally penetrate unripe strawberries through wounds or natural openings and remain quiescent until ripe, causing rapid decay of fruit at or after harvest [4].…”

Section: Introductionmentioning

confidence: 99%

“…Synthetic fungicides, such as cyprodinil, phenylpyrrole, anilinopyrimidine, and fludioxonil have been used to manage gray mold on strawberries in the field; however, no strategy has been developed for managing postharvest infection [3]. These fungicides, with site-specific action, have been used for more than thirty years, leading to resistant fungal strains.…”

Section: Introductionmentioning

confidence: 99%

Antifungal Activities and Mode of Action of Cymbopogon citratus, Thymus vulgraris, and Origanum heracleoticum Essential Oil Vapors against Botrytis cinerea and Their Potential Application to Control Postharvest Strawberry Gray Mold

Yan

Chen

et al. 2021

Foods

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Gray mold caused by Botrytis cinerea is one of the most destructive postharvest decay of strawberry fruit. The present study aims to identify essential oils with antifungal activity against B. cinerea and the underlying mechanisms and their potential application in controlling postharvest decay. In the screening test, essential oils from Cymbopogon citratus (Cc), Thymus vulgraris (Tv), and Origanum heracleoticum (Oh) exhibited maximum inhibition of B. cinerea mycelial growth. The three essential oils altered the hyphal morphology and ultrastructure and resulted in many blebs around the hyphae. The essential oils damaged the plasma membrane of B. cinerea cells and resulted in the leakage of intercellular nucleic acids, proteins and soluble sugars. The exposure of strawberries to the vapors of these three essential oils in commercial package reduced gray mold, with Tv and Oh exhibiting strong efficiency and disease index reduction by 53.85% and 57.69%, respectively. Oh also inhibited postharvest decay and maintained fruit quality, preventing weight loss and soluble solid degradation. The study proposes using plant essential oils as an alternative to chemical fungicides in controlling the gray mold of strawberries.

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Resistance response enhancement and reduction of Botrytis cinerea infection in strawberry fruit by Morchella conica mycelial extract

Cited by 19 publications

References 48 publications

Characterization of the Mechanism of Action of Serratia rubidaea Mar61-01 against Botrytis cinerea in Strawberries

Characterization of the Mechanism of Action of Serratia rubidaea Mar61-01 against Botrytis cinerea in Strawberries

Postharvest quality maintenance of wax apple and guava fruits by use of a fermented broth of an ε-poly-l-lysine-producing Streptomyces strain

Antifungal Activities and Mode of Action of Cymbopogon citratus, Thymus vulgraris, and Origanum heracleoticum Essential Oil Vapors against Botrytis cinerea and Their Potential Application to Control Postharvest Strawberry Gray Mold

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