Resistance pattern and distribution of carbapenemase and antiseptic resistance genes among multidrug-resistant Acinetobacter baumannii isolated from intensive care unit patients

Shirmohammadlou, Neda; Zeighami, Habib; Haghi, Fakhri; Kashefieh, Mehdi

doi:10.1099/jmm.0.000826

Cited by 34 publications

(34 citation statements)

References 31 publications

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“…According to our previous study [2], all A. baumannii isolates were resistant to three or more antimicrobial agents and considered as MDR. The highest rate of resistance was detected against ciprofloxacin and imipenem (100%), followed by piperacillin (99%) and cefepime/levofloxacin/ceftazidime (97%).…”

Section: Resultsmentioning

confidence: 99%

“…In our previous study, a total of 100 non-replicate A. baumannii clinical isolates were randomly recovered from different specimens including blood, sputum, wound swabs, chest tube secretions and urine from immunocompromised patients with symptomatic clinical infections at least 48 h after ICU admission [2]. Case patients were defined as patients infected by A. baumannii according to the Centers for Disease Control and Prevention criteria.…”

Section: Methodsmentioning

confidence: 99%

“…The antibiotic susceptibility was determined using the disk diffusion method in our previous study [2]. The following antimicrobial disks were used: ampicillin-sulbactam (10/10 μg ), ceftazidim (30 μg), imipenem (10 μg), gentamicin (10 μg), tobramycin (10 μg), doxycycline (30 μg), ciprofloxacin (5 μg), levofloxacin (5 μg), co-trimoxazole (1.25/23.75 μg), piperacillin (100 μg) and cefepime (30 μg) (MAST, Merseyside, U.K).…”

Section: Methodsmentioning

confidence: 99%

“…Acinetobacter baumannii is an important opportunistic human pathogen that causes a variety of infections as ventilator-associated pneumonia, meningitis, bacteremia, wound and soft-tissue infections, peritonitis and urinary tract infections [1, 2]. High prevalence of multidrug resistant (MDR) A. baumannii has emerged as serious problem in healthcare settings in Iran [2, 3]. Persistence and survival ability of MDR A. baumannii in various hospital environments and dry condition has made it as a major cause of nosocomial infections worldwide [4].…”

Section: Introductionmentioning

confidence: 99%

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Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients

et al. 2019

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Background Nosocomial infections and persistence of multidrug resistant biofilm forming Acinetobacter baumannii in hospitals has made it as a serious problem in healthcare settings worldwide. Methods A total of 100 A. baumannii clinical isolates from immunocompromised patients hospitalized in ICU were investigated for biofilm formation, the presence of biofilm related genes ( bap, ompA, csuE, fimH, epsA, bla PER-1 , bfmS, ptk, pgaB, csgA, kpsMII ), integron characterization and molecular typing based on REP-PCR. Results All isolates were resistant to three or more categories of antibiotics and considered as multidrug resistant (MDR). A total of 32 isolates were resistant to all tested antibiotics and 91% were extensively drug-resistance (XDR). All isolates were able to produce biofilm and 58% of isolates showed strong ability to biofilm formation. All strong biofilm forming A. baumannii isolates were XDR. All A. baumannii isolates carried at least one biofilm related gene. The most prevalent gene was csuE (100%), followed by pgaB (98%), epsA and ptk (95%), bfmS (92%) and ompA (81%). 98% of isolates carried more than 4 biofilm related genes, simultaneously. Class I integron (67%) was more frequent in comparison with class II (10%) ( P < 0.05). The REP-PCR patterns were classified as 8 types (A-H) and 21 subtypes. The A1 (23%) and C1 (15%) clusters were the most prevalent among A. baumannii isolates ( P < 0.05). According to the REP-PCR patterns, 23% of all isolates had a clonal relatedness. Conclusion Our study revealed the high frequency of biofilm forming XDR A. baumannii in ICU patients, with a high prevalence of biofilm related genes of csuE and pgaB. It seems that the appropriate surveillance and control measures are essential to prevent the emergence and transmission of XDR A. baumannii in our country.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients

et al. 2019

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“…In 2017, SENTRY Antimicrobial Surveillance Program reported the highest number of MDR strains of A. baumannii in Europe and Latin America, Asia-Pacific, and North America between 1997 and 2016 (24). Also, high prevalence of MDR A. baumannii has emerged as a serious problem in healthcare settings in Iran (25). Previous studies have shown that fluoroquinolones are one of the first-line therapies for A. baumannii infections (26).…”

Section: Discussionmentioning

confidence: 99%

Phenotypic Activity of Efflux Pumps by Carbonyl Cyanide M-Chlorophenyl Hydrazone (CCCP) and Mutations in GyrA and ParC Genes Among Ciprofloxacin-Resistant Acinetobacter baumannii Isolates

Jahromy

Ranjbar

Pishkar

2020

Jundishapur J Microbiol

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Background: Acinetobacter baumannii is one of the major bacteria causing nosocomial infections. The emergence of multidrugresistant (MDR) isolates causes morbidity and mortality and lead to financial burdens for patients and public health systems. Objectives: Regarding the use of ciprofloxacin and increase of ciprofloxacin resistance, this study was aimed to investigate the role of the efflux pump and mutations in gyrA and parC genes as the mechanisms of ciprofloxacin-resistance. Methods: This study was conducted on 55 strains of A. baumannii isolated from the patients hospitalized in Milad Hospital, Tehran. The isolates were identified by biochemical tests, and their antibiotic susceptibility was assessed by the disc diffusion method. To investigate the role of the efflux pump, minimum inhibitory concentration (MIC) of ciprofloxacin was determined in the presence and absence of the carbonyl cyanide 3-chloro phenyl hydrazine (CCCP) inhibitor. The PCR was used for amplification of gyrA, and parC genes and sequencing of its products was carried out to track the mutations. Results: The highest and lowest antibiotic resistance were observed in ciprofloxacin (100%) and tobramycin (52.7%), respectively. Moreover, 96% of the A. baumannii isolates exhibited MDR. Five percent of strains showed 4-fold MIC reduction after the use of the inhibitor and were reported as the strains with high activity efflux pump. In 100% of the isolates, gyrA and parC genes were detected, and only one mutation was observed in parC and gyrA genes in the studied isolates that altered amino acid type. Conclusions: Reduction in MIC in the presence of an efflux pump inhibitor confirmed its role in enhancing the resistance to ciprofloxacin in some isolates. The results of this study also revealed no significant relationship between the ciprofloxacin resistance of the studied isolates and mutation in the encoding region of parC and gyrA genes. Thus, the significance of other mechanisms, such as the expression of genes encoding efflux pump proteins, should not be neglected.

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Molecular Typing of Multidrug-Resistant Acinetobacter baumannii Isolates from Clinical Specimens by ERIC-PCR and MLVA

Osanloo,

Zeighami,

Haghi

et al. 2023

Curr Microbiol

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Resistance pattern and distribution of carbapenemase and antiseptic resistance genes among multidrug-resistant Acinetobacter baumannii isolated from intensive care unit patients

Cited by 34 publications

References 31 publications

Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients

Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients

Phenotypic Activity of Efflux Pumps by Carbonyl Cyanide M-Chlorophenyl Hydrazone (CCCP) and Mutations in GyrA and ParC Genes Among Ciprofloxacin-Resistant Acinetobacter baumannii Isolates

Molecular Typing of Multidrug-Resistant Acinetobacter baumannii Isolates from Clinical Specimens by ERIC-PCR and MLVA

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