Resistance of winter wheat varieties registered in the Czech Republic to mycotoxin accumulation in grain following inoculation with Fusarium culmorum

Chrpová, J.; Šíp, V.; Matějová, Eva; Sýkorová, S.

doi:10.17221/1910-cjgpb

Cited by 23 publications

(14 citation statements)

References 16 publications

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“…It is evident that a high disease incidence was recorded in 2006, which could be characterized by relatively high temperatures following the inoculation. High temperatures accompanied by sufficient humidity (when using the mist irrigation of plots) favoured early FHB development in the wheat spike and resulted in the high accumulation of DON (Chrpová et al 2007). These conditions appeared to be favourable for all examined isolates (demonstrated for DON content in Figure 1) and especially for the highly pathogenic ones (regression coefficient b = 1.97).…”

Section: Evaluation Of Year Cultivar and Isolate (Species) Effects Omentioning

confidence: 95%

“…Relatively large differences in the magnitude of correlation coefficients in different years (r = 0.45-0.99) are the main reason for inclusion of all traits in the analysis. Both FDK and TGW-R measuring the kernel infection can be considered as valuable traits (Šíp et al 2002), but FDK is more frequently used as an indicator of FHB resistance (Arseniuk et al 1999;Ittu et al 2000;Wisniewska et al 2002;Lemmens et al 2003;Mesterházy et al 2005;Chrpová et al 2007). The importance of FDK for the classification of cultivar resistance is documented here by the highest cultivar F values from analyses of variance (Tables 1 and 4), relatively higher number of detected significant differences between the cultivars (Table 7) as well as by the clearest separation of Arina from the other cultivars (Figure 1c).…”

Section: Aggressiveness and Toxin Production Abilitymentioning

confidence: 99%

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Assessing resistance to head blight in wheat cultivars inoculated with different Fusarium isolates

Šíp¹,

Chrpová²,

Sýkorová³

2008

CZECH J GENET PLANT

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Four winter wheat cultivars, differing in resistance to Fusarium head blight (FHB), were spray inoculated under field conditions for three years with 18 isolates of two Fusarium species (F. graminearum and F. culmorum) obtained from different regions of the Czech Republic. Deoxynivalenol (DON) contamination, FHB severity (VSS), Fusarium damaged kernels (FDK) and reductions of thousand grain weight and grain weight per spike were measured to describe the aggressiveness level of isolates. Highly significant correlations were found between these traits, but correlation coefficients ranged from 0.45 to 0.99. Analyses of variance showed significant effects of year, cultivar, isolate and Fusarium species on all traits. Experimental year accounted for the highest proportion of variation (37-59%), followed by cultivar (11-39%), isolate (9-18%) and species (1-3%). Two-way and three-way interactions between the main factors were also statistically significant, but with generally lower contribution to total variation. Though the examined isolates of two Fusarium species differed largely in quantitative aggressiveness, they did not show any evident qualitative differences in virulence. Pathogenic specialization of isolates was relatively higher in FDK. This trait appeared to be less affected by the disease level than the content of DON, which strongly reflected environmental conditions, cultivar resistance and pathogenicity of an isolate. Under highly variable weather conditions the significance of differences between the cultivars was not often detected by a single isolate and it was necessary to look for isolate groups in order to get a higher percentage of significant differences and prove the moderate resistance of the cultivar Arina. The highest number of significant differences between the cultivars was reached after selection of isolates, the application of which resulted in pronounced cultivar effects (lower interaction with years) and which showed high correspondence with the average reaction of cultivars in different years (r 2 ). Divergence in sensitivity to the environment could be another criterion for selecting sources of inoculum.

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Section: Evaluation Of Year Cultivar and Isolate (Species) Effects Omentioning

confidence: 95%

Section: Aggressiveness and Toxin Production Abilitymentioning

confidence: 99%

Assessing resistance to head blight in wheat cultivars inoculated with different Fusarium isolates

Šíp¹,

Chrpová²,

Sýkorová³

2008

CZECH J GENET PLANT

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“…The Swiss variety Arina, generally known as a source of moderate resistance to FHB (Jenny et al 2000), was used in these experiments as a check. The registered varieties were bred in 6 European countries (12 -Czech Republic, 12 -Germany, 2 -France, 2 -Austria, 1 -the Netherlands and 1 -Denmark (Šíp et al 2002;Chrpová et al 2007) was performed at mid-flowering (GS 64: anthesis half-way) (Zadoks et al 1974). One term spraying of inoculum (conidial suspension 0.8 × 10 7 ml) onto bunches of 10 flowering spikes randomly selected within hill plots was applied.…”

Section: Methodsmentioning

confidence: 99%

Resistance of winter wheat varieties registered in the Czech Republic to Fusarium head blight in relation to the presence of specific Rht Alleles

Chrpová¹,

Šíp²,

Štočková³

et al. 2010

CZECH J GENET PLANT

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Abstract:Resistance of 31 winter wheat varieties (bred in 6 European countries) to Fusarium head blight (FHB) was evaluated in field trials lasting for three years (2007, 2008, 2009) after artificial inoculation with Fusarium culmorum. The data on deoxynivalenol (DON) content were supplemented by symptom scores and determination of % of Fusarium damaged grains and % reductions of thousand-grain weight and grain weight per spike due to infection. These varieties and also 120 advanced breeding lines from the Úhřetice Breeding Station, SELGEN company were examined for the presence of gibberellic acid (GA) insensitive semi-dwarfing genes to evaluate their effect on FHB. The conditions of experimental years highly influenced the performance of all characters. The highest production of DON occurred in 2008 at a high temperature and high moisture content during the infection period. In all years the moderate resistance to accumulation of DON (at the level of Swiss variety Arina) was detected in the Czech varieties Bakfis, Federer, Baletka, Samanta and Sakura. Among these varieties, Federer showed a low accumulation of DON at a relatively higher symptom expression and greater reduction of grain weight per spike, but the other four varieties and the reference variety Arina expressed resistance in all the examined traits. The varieties Pitbull, Cubus, Kodex and Bagou were found to be highly susceptible to FHB. The presence of the dwarfing allele rht-D1b resulted in a significantly higher mean symptom score and also in a higher affection of the other traits. Nonetheless, the analysis of frequency distributions in symptom scores showed the presence of resistant lines also among the GA insensitive lines, but with a lower frequency than in the group of GA sensitive genotypes. A relatively greater effect on manifestation of the disease had plant height, and therefore it is suggested that the adverse impact of rht-D1b on FHB resistance could be to a high degree excluded by opting for taller rht-D1b genotypes.

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“…Seeds from infected spikes were analysed for DON content as determined by ELISA using RIDASCREEN ® FAST DON kits from R-Biopharm GmbH, Darmstadt, Germany. DON content determination is described in detail by Chrpová et al (2007).…”

Section: Cereal Research Communicationsmentioning

confidence: 99%

Combining ability analysis of fusarium head blight resistance in European winter wheat varieties

Šíp

Chrpová

Štěrbová

et al. 2017

Cereal Research Communications

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The aims of the present study were to estimate the general combining ability (GCA) and the specific combining ability (SCA) effects controlling type II FHB resistance across environments in a set of European winter wheat varieties and, for purposes of future selection, to identify potential combinations of parents with suitable levels of FHB resistance. Parental varieties as well as F 1 generations were evaluated under both field and greenhouse conditions in two years. The results of the present study indicate that in the F 1 generation mean DON content was relatively lowest after crossing of moderately resistant parents (Sakura/Bakfis, Sakura/Federer, Petrus/Bakfis, and Sakura/Petrus), and mean DON content is low also after crossing the moderately resistant Bakfis variety with the susceptible Biscay and Cubus varieties. Evaluation of crosses in the F 1 generation was followed by evaluation of selected crosses (derived from the Bakfis and Sakura varieties) in the F 2 generation. Correlations between F 1 and F 2 were highly significant in relation both to their DON content and visual symptom score (VSS), as well as between the individual experiments (and in the different years). The only exception was in the case of the 2014 field experiment, when inoculation was successful but conditions were not optimal for the disease to progress and DON to accumulate. The selection of a suitable parental variety (with a high GCA) can markedly influence the success rate of breeding for resistance to FHB. Detection of high SCA in the F 1 generation is important for directing breeders to promising combinations for achieving FHB resistance. It was demonstrated here that low DON content may be achieved even after crossing a moderately resistant variety with susceptible varieties. Another possibility is to make use of heterosis directly for acquiring resistance in hybrid wheat (for decreasing DON content and manifestation of symptoms).

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Resistance of winter wheat varieties registered in the Czech Republic to mycotoxin accumulation in grain following inoculation with Fusarium culmorum

Cited by 23 publications

References 16 publications

Assessing resistance to head blight in wheat cultivars inoculated with different Fusarium isolates

Assessing resistance to head blight in wheat cultivars inoculated with different Fusarium isolates

Resistance of winter wheat varieties registered in the Czech Republic to Fusarium head blight in relation to the presence of specific Rht Alleles

Combining ability analysis of fusarium head blight resistance in European winter wheat varieties

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