Resistance of Transgenic <i>Prunus domestica</i> to Plum Pox Virus Infection

Ravelonandro, Michel; Scorza, R.; Bachelier, J. C.; Labonne, Gérard; Lévy, L.; Damsteegt, V. D.; Callahan, Ann M.; Dunez, Jean

doi:10.1094/pdis.1997.81.11.1231

Cited by 103 publications

(94 citation statements)

References 36 publications

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“…This is in agreement with the results obtained in France and Romania (Ravelonandro et al 1997(Ravelonandro et al , 2002, and in Spain and Poland (Malinowski et al 2006). Original results were obtained when graft inoculated trees were exposed to a very high infection pressure with IB being allowed to reach the size of 20-30% of the supporting HoneySweet tree (Figure 2).…”

Section: Resultssupporting

confidence: 91%

“…Sharka disease is the most devastating disease of plum and is responsible for the reduction or loss of the plum production in many areas of Europe ). In the past 22 years of research by an international team of public sector scientists, a GM plum highly resistant to PPV was developed and thoroughly tested in the greenhouse and field, in the USA and Europe -the Czech Republic, France, Poland, Romania, and Spain for the resistance to PPV and for environmental safety (Scorza et al 1994;Ravelonandro et al 1997Ravelonandro et al , 2000Ravelonandro et al , 2002Hily et al 2004Hily et al , 2007Polák et al 2005Polák et al , 2008aMalinowski et al 2006;Capote et al 2007Capote et al , 2008Zagrai et al 2008aZagrai et al ,b, 2010). An original trial of a high and permanent infection pressure of PPV-Rec alone and in combinations with Prune dwarf virus (PDV) and Apple chlorotic leafspot virus (ACLSV) was initiated in the Czech Republic (Polák et al 2008a,b).…”

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confidence: 99%

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Biotech/GM crops in horticulture: plum cv. HoneySweet resistant to Plum pox virus

Polák¹,

Kumar²,

Krška³

et al. 2012

Plant Prot. Sci.

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Polák J., Kumar J., Krška B., Ravelonandro M. (2012): Biotech/GM crops in horticulture: Plum cv. HoneySweet resistant to Plum pox virus. Plant Protect. Sci., 48 (Special Issue): S43-S48.Commercialisation of Biotech/GM (Biotech) crops started in 1995. Not only field crops, but also horticultural transgenic crops are under development and are beginning to be commercialised. Genetic engineering has the potential to revolutionise fruit tree breeding. The development of transgenic fruit cultivars is in progress. Over the past 20 years an international public sector research team has collaborated in the development of HoneySweet plum which is highly resistant to Plum pox virus (PPV) the most devastating disease of plums and other stone fruits. HoneySweet was deregulated in the USA in 2010. HoneySweet (aka C5) has been evaluated for eleven years (2002)(2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010)(2011)(2012) in a regulated field trial in the Czech Republic for the resistance to PPV, Prune dwarf virus (PDV), and Apple chlorotic leaf spot virus (ACLSV), all of them being serious diseases of plum. Even under the high and permanent infection pressure produced through grafting, PPV has only been detected in HoneySweet trees in several leaves and fruits situated close to the point of inoculum grafting. The lack of infection spread in HoneySweet demonstrates its high level of PPV resistance. Co-infections of PPV with PDV and/or ACLSV had practically no influence on the quantity and quality of HoneySweet fruit which are large, sweet, and of a high eating quality. In many respects, they are superior to the fruits of the well-known cultivar Stanley. Many fruit growers and fruit tree nurseries in the Czech Republic are supportive of the deregulation of HoneySweet plum to help improve the plum production and control the spread of PPV.

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Section: Resultssupporting

confidence: 91%

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confidence: 99%

Biotech/GM crops in horticulture: plum cv. HoneySweet resistant to Plum pox virus

Polák¹,

Kumar²,

Krška³

et al. 2012

Plant Prot. Sci.

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“…HoneySweet) [6]. "HoneySweet" plum has proven to be highly resistant to PPV [7] and this high level of resistance has remained stable in European field tests for over 15 years under high PPV infection pressure [8]. Resistance in "HoneySweet" is based on gene silencing or RNA interference (RNAi) which is a natural virus defense system in plants [9].…”

Section: Introductionmentioning

confidence: 99%

"HoneySweet" Plum—A Valuable Genetically Engineered Fruit-Tree Cultivar

Ravelonandro¹,

Scorza²,

Polák³

et al. 2013

FNS

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ABSTRACT"HoneySweet" is a plum variety developed through genetic engineering to be highly resistant to plum pox potyvirus (PPV) the causal agent of sharka disease that threatens stone-fruit industries world-wide, and most specifically in Europe. Field testing for over 15 years in Europe has demonstrated the stable and durable PPV resistance of "HoneySweet". Resistance is based on gene silencing whereby the inserted gene induces a natural plant defense mechanism against viruses. This resistance has been transferred to seedlings through cross-hybridization as a single locus dominant trait making it useful as a parent for developing new plum varieties for specific growing areas and markets. "HoneySweet" plums are of high quality and compare well to the quality and nutritional value of conventional plums. "HoneySweet" demonstrates the utilization of genetic engineering to provide safe and effective solutions to important agricultural challenges facing growers, and ultimately consumers.

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“…In both cases, transgenic plants were generated with the aim of overexpressing the CP transgene, thus getting an ectopic overaccumulation of the corresponding protein that would reencapsidate the challenging virus soon after initiating infection. However, only unique transgenic events with several CP transgene insertions showed strong resistance to the challenging viruses Ravelonandro et al 1997), particularly in field trial assays (Hily et al 2004). Molecular analysis of these events revealed very low transgene mRNA levels and undetectable CP accumulation ).…”

Section: Ctv and Rna Silencingmentioning

confidence: 99%

Transgenic resistance against Citrus tristeza virus (CTV) and analysis of the viral p23 protein as pathogenicity determinant in citrus.

Calvo¹

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AgradecimientosMe gustaría agradecer en primer lugar a Leandro Peña y al Instituto Valenciano de Investigaciones Agrarias por darme la oportunidad de realizar la Tesis doctoral en el Laboratorio de Transformación genética.Quiero agradecer a todo el equipo de Cultivo In vitro, a Pepe, Juana Mari, Cloti, Carmen, Pablo, Toni, Vio, etc., por todo el apoyo, sus consejos y sus enseñanzas.A todo el laboratorio de Leandras: los postdoc Magda, Mamen y Juan, por toda la ayuda y enseñanzas que me disteis; a Joseph, por cuidar tan bien de nuestras plantitas; y como no, agradecer a mis chicas: Ana, Ali, Elsa, Berta, Montse y Ana cubana; además de todos los compañeros de pasillo ivias/ex-ivias: Jesús, Vero, Andrés, Frank, Marta, Jose, Diana, Ezequiel; los que ya se fueron: Inma, Lucía, Nubi, Ague, Marta, Rosa, Esther, Giovanni, Jorge, María, Mari Cruz… por todo el apoyo, el cariño, las risas, los consejos, los cafés, las alquerías, las escapadas, los lunes al sol, … mil gracias, porque ahora son grandes amigos con los que he compartido tantos y tantos buenos momentos.A los que he olvidado nombrar, gracias también.Y por último, gracias a mi familia, porque sin ellos nada de esto sería posible. ABSTRACTCitrus tristeza virus (CTV) is the causal agent of one of the most devastating viral diseases of citrus trees in the world. CTV is phloem-restricted in natural citrus hosts, and has evolved three silencing suppressor proteins acting at intra-(p23 and p20) and intercellular level (p20 and p25) to overcome strong host antiviral defense in citrus. RNA interference (RNAi), an approach based on using dsRNA to trigger RNA silencing, has been widely used for generating transgenic plants resistant against viruses. Considering the important role of p23, p20 and p25 in CTV pathogenesis, we have transformed Mexican lime plants with an intron-hairpin vector carrying full untranslatable versions of genes p25, p20, p23 and the 3'-UTR from the CTV strain T36, to attempt silencing their expression in CTVinfected cells. Complete resistance to viral infection was observed in three transgenic lines, with all their propagations remaining symptomless and virus-free after graft-inoculation with CTV-T36, either in the non-transgenic rootstock or directly in the transgenic scion. Accumulation of transgene-derived siRNAs was necessary but not sufficient for CTV resistance. Challenging immune transformants with a divergent CTV strain resulted in partial breakage of the resistance, stressing the importance of sequence identity in the underlying RNAi mechanism. This is the first evidence that it is possible to achieve full resistance to CTV in a highly sensitive citrus host by targeting simultaneously its three viral silencing suppressors through RNAi. The p23 protein encoded by the virus is additionally an important pathogenicity factor. Ectopic expression of p23 in RESUMEN El virus de la tristeza de los cítricos (Citrus tristeza virus;CTV) es el agente causal de unas de las enfermedades virales de los árboles cítricos más devastadoras en el mundo. CTV es...

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Resistance of Transgenic Prunus domestica to Plum Pox Virus Infection

Cited by 103 publications

References 36 publications

Biotech/GM crops in horticulture: plum cv. HoneySweet resistant to Plum pox virus

Biotech/GM crops in horticulture: plum cv. HoneySweet resistant to Plum pox virus

"HoneySweet" Plum—A Valuable Genetically Engineered Fruit-Tree Cultivar

Transgenic resistance against Citrus tristeza virus (CTV) and analysis of the viral p23 protein as pathogenicity determinant in citrus.

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Resistance of Transgenic Prunus domestica to Plum Pox Virus Infection

Cited by 103 publications

References 36 publications

Biotech/GM crops in horticulture: plum cv. HoneySweet resistant to Plum pox virus

Biotech/GM crops in horticulture: plum cv. HoneySweet resistant to Plum pox virus

&quot;HoneySweet&quot; Plum—A Valuable Genetically Engineered Fruit-Tree Cultivar

Transgenic resistance against Citrus tristeza virus (CTV) and analysis of the viral p23 protein as pathogenicity determinant in citrus.

Contact Info

Product

Resources

About

"HoneySweet" Plum—A Valuable Genetically Engineered Fruit-Tree Cultivar