Resistance of the Meth A sarcoma-associated rejection antigen to inactivation with glutaraldehyde

Price, Michael R.; Gerlier, Denis; Law, Lloyd W.

doi:10.1038/bjc.1981.230

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…Before conducting the biochemical analysis of the active molecules on HSV-Meth A, we had to determine the native immunogenic structure presented by HSV-Meth A. Therefore we treated HSV-Meth A with either glutaraldehyde as described by Price et al (14) or by heating at 56 C in order to preserve the effective molecules on the cell membrane and then used them for the CTL stimulation. The activity shown by GA-and HT-HSV-Meth A implied that the active moiety was the molecules on the plasma membrane (Fig.…”

Section: Discussionmentioning

confidence: 99%

Preparation of Membrane Fraction from Herpes Simplex Virus‐Infected Cells which Induce Cytotoxic T Lymphocytes

Hitsumoto

Sonoda²,

Okuyama³

et al. 1983

Microbiology and Immunology

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The immunogenic capacity of herpes simplex virs (HSV)-infected cells and their subcellular membrane fractions was investigated by assessing the anti-HSV cytotoxic T lymphocyte (CTL) response in cultures of spleen lymphocytes from HSV-primed BALBJc mice. Methylchloranthrane-induced fibrosarcoma (Meth A) cells infected with HSV (HSV-Meth A) were fixed either with glutaraldehyde or by heating at 56 C to preserve their immunogenic competence and then used as a stimulator. Microsomes and plasma membranes were prepared from HSV-Meth A and their immunogenic activities were determined. Though the recovery of stimulatory activity in the plasma membrane fraction was half of that in the microsome fraction, the activity in the former was much more stable than in the latter and the plasma membrane fraction proved to be well qualified as an immunogen for anti-HSV CTL induction. Upon purification, the specific activity of the membrane fraction, on the basis of protein concentration, increased 43-fold.Anti-herpes simplex virus cytotoxic T lymphocytes (anti-HSV CTL) are presumed to suppress HSV growth in the early phase of infection and to prevent the secondary outgrowth of HSV in the recurrent disease (12). In fact, enhanced activity of anti-HSV CTL has been noted in post-herpetic patients (17,18,20), as well as in the animal model system of HSV infection (5, 9, 22). For induction of CTL, inoculation oflive virus has been employed widely (25), but it is not feasible to use live HSV because of the oncogenic potency of the HSV genome (1, 6). It is therefore desirable to develop a simplified artificial immunogen specific for CTL induction. To this end, the attempt to reconstitute the immunogen by using HSV antigens and histocompatible membrane antigens appears to be promising (8), as described for other virus systems (4, 7).By virtue of the method previously devised for in vitro assay of anti-HSV CTL (22), it is now feasible to identity the immunogenic molecules of HSV and the requirements of major histocompatibility complex (MHC)-related molecules. In the present study, we reevaluated the immunogenicity of HSV-infected methyl-757

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Section: Discussionmentioning

confidence: 99%

Preparation of Membrane Fraction from Herpes Simplex Virus‐Infected Cells which Induce Cytotoxic T Lymphocytes

Hitsumoto

Sonoda²,

Okuyama³

et al. 1983

Microbiology and Immunology

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Use of an automatic cell harvester in a cellular radioimmunoassay

Gerlier¹,

Avice²

1984

Journal of Immunological Methods

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A Critical Review of the Toxicology of Glutaraldehyde

et al. 1992

Critical Reviews in Toxicology

187

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Glutaraldehyde, a low molecular weight aldehyde, has been investigated for toxicity in humans and animals. Examination of this dialdehyde was indicated from previous studies with other aldehydes in which carcinogenicity of formaldehyde and toxicity of acetaldehyde and malonaldehyde have been disclosed. Information gaps concerning the actions of glutaraldehyde have been identified in this review and recommendations are suggested for additional short- and long-term studies. In particular, information regarding irritation of the respiratory tract, potential neurotoxicity, and developmental effects would assist in a complete hazard evaluation of glutaraldehyde. Further study related to disposition, metabolism, and reactions of glutaraldehyde may elucidate the mechanism of action.

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Resistance of the Meth A sarcoma-associated rejection antigen to inactivation with glutaraldehyde

Cited by 3 publications

References 6 publications

Preparation of Membrane Fraction from Herpes Simplex Virus‐Infected Cells which Induce Cytotoxic T Lymphocytes

Preparation of Membrane Fraction from Herpes Simplex Virus‐Infected Cells which Induce Cytotoxic T Lymphocytes

Use of an automatic cell harvester in a cellular radioimmunoassay

A Critical Review of the Toxicology of Glutaraldehyde

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