Resistance of QYm.nau-2D to wheat yellow mosaic virus was derived from an alien introgression into common wheat

Chen, Yiming; Ji, Jialun; Kong, Dehui; Tang, Xiong; Wen, Mingxing; Wang, Guoqing; Dai, Keli; Shi, Peiyao; Zhang, Xu; Zhang, Huajian; Jiao, Chengzhi; Wang, Zongkuan; Sun, Li; Yuan, Chunxia; Wang, Haiyan; Zhang, Xueyong; Sun, Bing‐Jian; Fei, Xinru; Guo, Hong; Xiao, Jin; Wang, Xiue

doi:10.1007/s00122-023-04286-1

Cited by 2 publications

(15 citation statements)

References 72 publications

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“…Our laboratory research showed that WYMV P1 protein also has viral suppressor of RNA silencing (VSR) activity, and plays a suppressor role by interfering with calmodulin-related antiviral RNAi defense, confirming that its VSR activity contributes to the systemic infection of WYMV in wheat (Chen et al, 2023). The P2 proteins of BaMMV, OMV, and BaYMV may be related to fungal transmission (Jacobi et al, 1995;Peerenboom et al, 1992;Zheng et al, 2002).…”

Section: Introductionmentioning

confidence: 79%

“…Agrobacterium co-infiltration experiments showed that P2 had relatively strong local VSR activity in N. benthamiana leaves at the four-leaf stage, but weak at the eightleaf stage (Figure 1B and C). The WYMV P2 was similar to P1 in that it had similar local VSR activity and only exhibited strong suppressor activity in the younger N. benthamiana leaves, which further explains why wheat seedlings need to be inoculated with WYMV at the one to two leaf stage (Chen et al, 2023;Zhang et al, 2021;Shang et al, 2002). One significant difference between them was that the P2 truncated mutants had local VSR activity (Figure 2A-C), while the P1 truncated mutants tested lost local VSR activity (Chen et al, 2023).…”

Section: Discussionmentioning

confidence: 98%

“…The WYMV P2 was similar to P1 in that it had similar local VSR activity and only exhibited strong suppressor activity in the younger N. benthamiana leaves, which further explains why wheat seedlings need to be inoculated with WYMV at the one to two leaf stage (Chen et al, 2023;Zhang et al, 2021;Shang et al, 2002). One significant difference between them was that the P2 truncated mutants had local VSR activity (Figure 2A-C), while the P1 truncated mutants tested lost local VSR activity (Chen et al, 2023). Amino acid sequence alignment of Bymovirus P2 revealed 13 conserved amino acid residues (Figure 2D), and VSR activity screening of the 13 point mutants showed that conserved proline 70 and glycine 230 in P2 were essential for RNA-silencing suppressor activity (Figure 2E and F).…”

Section: Discussionmentioning

confidence: 98%

“…Viruses have developed a variety of mechanisms to overcome antiviral RNA silencing. In general, the mechanisms of viral silencing suppressors can be divided into two broad categories in the downstream of the RNA-silencing pathway (Brodersen and Voinnet, 2006;Chen et al, 2023;Li et al, 2019;Marco et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…The qRT-PCR results showed that the gene for CaM was the most significantly downregulated; it was recently reported that VSRs exert suppressor activity by directly interacting with CaM, thereby interfering with the CaM-CAMTA3 interaction (Chen et al, 2023;Wang et al, 2021;Wang et al, 2022). Therefore, Y2H and BiFC were used to verify the P2-CaM interaction (Figure 3; Supporting Figure 7).…”

Section: Wymv P2 Interacts With Gene-silencing Related Protein Nbcammentioning

confidence: 96%

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The P2 protein of wheat yellow mosaic virus acts as a viral suppressor of RNA silencing to facilitate virus infection in wheat plants

Chen,

Zhang,

et al. 2023

Preprint

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Wheat yellow mosaic virus (WYMV) causes severe viral wheat disease in Asia. The WYMV P1 protein encoded by RNA2 has viral suppressor of RNA silencing (VSR) activity to facilitate virus infection; however, VSR activity has not been identified for P2 protein encoded by RNA2. In this study, P2 protein exhibited strong VSR activity inNicotiana benthamianaat the four-leaf stage, and point mutants P70A and G230A lost VSR activity. Protein P2 interacted with calmodulin (CaM) protein, a gene-silencing associated protein, while point mutants P70A and G230A did not interact with it. Competitive bimolecular fluorescence complementation and competitive co-immunoprecipitation experiments showed that P2 interfered with the interaction between CaM and calmodulin-binding transcription activator 3 (CAMTA3), but the point mutants P70A and G230A could not. Mechanical inoculation of wheat within vitrotranscripts of WYMV infectious cDNA clone further confirmed that VSR-deficient mutants P70A and G230A decreased WYMV infection in wheat plants compared with the wild type. In addition, RNA silencing, temperature, and autophagy had significant effects on accumulation of P2 protein inN. benthamianaleaves. In conclusion, WYMV P2 plays a VSR role in wheat and promotes virus infection by interfering with calmodulin-related antiviral RNAi defense.One-sentence summaryWYMV P2 protein exerts VSR activity by interfering with the CaM–CAMTA3 interaction to facilitate virus efficient systemic infection in wheat plants.

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Section: Introductionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Wymv P2 Interacts With Gene-silencing Related Protein Nbcammentioning

confidence: 96%

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The P2 protein of wheat yellow mosaic virus acts as a viral suppressor of RNA silencing to facilitate virus infection in wheat plants

Chen,

Zhang,

et al. 2023

Preprint

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Identification of Barley yellow mosaic virus Isolates Breaking rym3 Resistance in Japan

Zhu,

Okiyama,

Mishina

et al. 2024

Genes

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In early spring 2018, significant mosaic disease symptoms were observed for the first time on barley leaves (Hordeum vulgare L., cv. New Sachiho Golden) in Takanezawa, Tochigi Prefecture, Japan. This cultivar carries the resistance gene rym3 (rym; resistance to yellow mosaic). Through RNA-seq analysis, Barley yellow mosaic virus (BaYMV-Takanezawa) was identified in the roots of all five plants (T01–T05) in the field. Phylogenetic analysis of RNA1, encompassing known BaYMV pathotypes I through V, revealed that it shares the same origin as isolate pathotype IV (BaYMV-Ohtawara pathotype). However, RNA2 analysis of isolates revealed the simultaneous presence of two distinct BaYMV isolates, BaYMV-Takanezawa-T01 (DRR552862, closely related to pathotype IV) and BaYMV-Takanezawa-T02 (DRR552863, closely related to pathotype III). The amino acid sequences of the BaYMV-Takanezawa isolates displayed variations, particularly in the VPg and N-terminal region of CP, containing mutations not found in other domains of the virus genome. Changes in the CI (RNA1 amino acid residue 459) and CP (RNA1 amino acid residue 2138) proteins correlated with pathogenicity. These findings underscore the importance of monitoring and understanding the genetic diversity of BaYMV for effective disease management strategies in crop breeding.

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Resistance of QYm.nau-2D to wheat yellow mosaic virus was derived from an alien introgression into common wheat

Cited by 2 publications

References 72 publications

The P2 protein of wheat yellow mosaic virus acts as a viral suppressor of RNA silencing to facilitate virus infection in wheat plants

The P2 protein of wheat yellow mosaic virus acts as a viral suppressor of RNA silencing to facilitate virus infection in wheat plants

Identification of Barley yellow mosaic virus Isolates Breaking rym3 Resistance in Japan

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