Resistance of potential probiotic lactic acid bacteria and bifidobacteria of African and European origin to antimicrobials: Determination and transferability of the resistance genes to other bacteria

Potencial probiótico in vitro de bactérias ácido-láticas isoladas de queijo-de-minas artesanal da Serra da Canastra, MG

“…A resistência à vancomicina em 100% das amostras de Lactobacillus e Weissella testadas foi também observada em outros trabalhos (Coppola et al, 2005;Ouoba et al, 2008;Rodríguez-Alonso et al, 2009), o que confirma que Lactobacillus e outros gêneros de bactérias ácido-láticas podem ter resistência intrínseca à vancomicina (Teuber et al, 1999 Em concordância com os resultados encontrados neste trabalho, diversos autores encontraram altas porcentagens de resistência à oxacilina em estudos que avaliaram a susceptibilidade a este antimicrobiano (Coppola et al, 2005;Rodríguez-Alonso et al, 2009). Mesmo pertencendo ao grupo dos β-lactâmicos, geralmente as taxas de resistência à oxacilina são maiores do que para penicilina (Teuber et al, 1999).…”

Assessment of Antibiotic Resistance in Probiotic Lactobacilli

“…In the diassociated model pAMβ1 has been transferred from L. reuteri to Enterococcus faecalis (Morelli et al, 1988). Interspecies conjugative transfer of tetracycline and erythromycin resistance plasmids from lactobacilli has been demonstrated previously in vitro (Gevers et al, 2003a;Ouoba et al, 2008;Feld et al, 2008 Transfer has been demonstrated in the GIT of rodents, both gnotobiotic (Feld et al, 2008;Jacobsen et al, 2007;Morelli et al, 1988) and those having an indigenous gut microbiota (Feld et al, 2008;Jacobsen et al, 2007;Schlundt et al, 1994;McConnell et al, 1991;Gruzza et al, 1994;Igimi et al, 1996). In addition, the in vivo transfer of vancomycin resistance has recently been shown between enterococci and probiotic lactobacilli in gnotobiotic mice (Mater et al, 2008).…”

“…Probiotic lactobacilli of African and European origins were studied and compared for their susceptibility to antibiotics. Acquired resistance genes encoding aminoglycoside (aph(3')-III, aadA, aadE) and tet(S) and erm(B) were detected (Ouoba et al, 2008). The potentially probiotic strain L. plantarum CCUG 43738, which displayed atypical phenotypic resistance to tetracycline and minocycline, was found to contain a tet(S) gene located on a plasmid of approximately 14 kb (Huys et al, 2006).…”

“…Transfer from lactobacilli to other commensal bacteria has been documented in vitro (Feld et al, 2008;Gevers et al, 2003b;Jacobsen et al, 2007;Sasaki et al, 1988;Schlundt et al, 1994). Studying the board-host-range conjugative plasmid pAMβ1, transfer was observed in vitro from lactobacilli (L. plantarum, L. reuteri, L. fermentum, and L. murinus) to other commensal bacteria (Ouoba et al, 2008;Tannock, 1987;Gasson & Davies, 1980;Shrago et al, 1986;West & Warner, 1985). In the diassociated model pAMβ1 has been transferred from L. reuteri to Enterococcus faecalis (Morelli et al, 1988).…”

Selection of Lactic Acid Bacteria as Probiotic Candidate for Chicken

“…Isolates of E5, E7, and E8 did not resistant to erythromycin, chloramphenicol, and tetracycline. Some species of LAB showed phenotypical sensitive to tetracycline and chloramphenicol, then intermediate to erythromycin but the gene encoding of erythromycin resistance was not found (Ouoba et al, 2008). According to FAO/WHO (2002) and EFSA (2012) probiotics as feed additives should not resistant to anti-biotics.…”