Resistance of Nicotiana tabacum to Phytophthora parasitica var. nicotianae Race 0 Is Enhanced by the Addition of N. plumbaginifolia Chromosome 9 with a Slight Effect on Host Genomic Expression

Abstract

Black shank is a devastating disease in tobacco production worldwide, and resistance to this disease in Nicotiana plumbaginifolia Viv. tobacco is controlled by the Php gene. The position of the Php gene on chromosomes of N. plumbaginifolia remains unclear. The tobacco line TP‐1, which was derived from N. tabacum L. cv. Yunyan87 and N. plumbaginifolia, was used in the present study to help locate the Php gene on the N. plumbaginifolia chromosomes and understand the effects of the N. plumbaginifolia chromosome addition on the genomic expression of N. tabacum. An in vitro infection test verified that TP‐1 was as resistant to Phytophthora parasitica var. nicotianae race 0 as N. plumbaginifolia and more resistant than Yunyan87. According to cytogenetic analysis, TP‐1 was identified as a monosomic alien addition line and the alien chromosome was recognized as the ninth chromosome of N. plumbaginifolia through. Thus, the P. parasitica var. nicotianae race 0 resistance gene might be Php on chromosome 9 of N. plumbaginifolia. A superficial transcriptomic analysis showed that addition of the alien chromosome slightly affected the genomic expression of the host, with only 3.32% of differentially expressed genes (DEGs) identified between TP‐1 and Yunyan87. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, most (9/13) genes classified as involved in plant–pathogen reactions, particularly five serine/threonine‐protein kinase genes, were upregulated in TP‐1. It is worth noting that flavonoid biosynthesis may be greatly downregulated in TP‐1: two genes were both downregulated in TP‐1 according to gene ontology analysis, and all 20 DEGs in the flavonoid biosynthesis pathway were downregulated in TP‐1 according to KEGG analysis.

NpPP2-B10, an F-Box-Nictaba Gene, Promotes Plant Growth and Resistance to Black Shank Disease Incited by Phytophthora nicotianae in Nicotiana tabacum

“…In previous studies, we analyzed the differentially expressed genes of the highly resistant tobacco species N. plumbaginifolia and of the moderately resistant cultivar ‘Yunyan 87’ identified by transcriptome sequencing [ 9 ]. A Unigene, c62451.graph_c0, was specifically expressed in N. plumbaginifolia.…”

“…Their resistance has been successfully transferred to cultivated tobacco by distant hybridization [ 6 ]. A study by Goins and Apple [ 7 ] showed that the resistance of N. longiflora and N. plumbaginifolia is regulated by the major genes Phl and Php , respectively, and the black shank resistance was introgressed into cultivated tobacco as early as 1960 [ 8 , 9 ]. In recent years, researchers have identified the glutathione S-transferase gene in N. tabacum as a negative regulator, and silencing of this gene has been found to promote resistance to black shank in tobacco [ 10 ].…”

Single-step genome-wide association study for susceptibility to Teratosphaeria nubilosa and precocity of vegetative phase change in Eucalyptus globulus

“…For instance, the disease resistance protein RPV1 is known to confer resistance to oomycete and fungal pathogens in cultivated grapevines ( Vitis vinifera) ( Gessler et al., 2011 ). LRR receptor-like serine/threonine-protein kinase GSO1 was reported to be related to the resistance of Nicotiana tabacum to the oomycete Phytophthora parasitica ( Dang et al., 2019 ). TMV resistance protein N-like was reported to be involved in the resistance in potato to the fungus Synchytrium endobioticum ( Hehl et al., 1999 ), and in peanut to two serious fungal foliar diseases ( Dang et al., 2021 ).…”

Single-step genome-wide association study for susceptibility toTeratosphaeria nubilosaand precocity of vegetative phase change inEucalyptus globulus

“…For instance, the disease resistance protein RPV1 is known to confer resistance to oomycete and fungal pathogens in cultivated grapevines ( Vitis vinifera) 69 . LRR receptor-like serine/threonine-protein kinase GSO1 was reported to be related to the resistance of Nicotiana tabacum to the oomycete Phytophthora parasitica 70 . TMV resistance protein N-like was reported to be involved in the resistance in potato to the fungus Synchytrium endobioticum 71 , and in peanut to two serious fungal foliar diseases 72 .…”