Resistance of <i>Haemophilus influenzae</i> Isolates in Children Under 5 Years Old with Acute Respiratory Infections in China between 2000 and 2002

Shen, XZ; Lu, Quan; Deng, Li; Yu, Sangjie; Zhang, H; Deng, Qiulian; Jiang, Min; Hu, Yang; Yang, Yucheng

doi:10.1177/147323000703500416

Cited by 6 publications

(6 citation statements)

References 35 publications

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“…22.7% of the strains produced β-lactamase. The incidence of β-lactamase production was only 12.0% in patients with acute upper respiratory tract infection under 5 years old in Shanghai, Guangzhou, and Beijing city from 2000 to 2002 (Shen et al, 2007). The incidence of β-lactamase producing strains manifested an ascending tendency in China from 2004China from to 2007 In this study, we confirmed a high heterogeneity of nontypeable H. influenzae strains (NTHi) by the MLST analysis.…”

Section: J Microbiolsupporting

confidence: 88%

Genotypic characteristics of Haemophilus influenzae isolates from pediatric pneumonia patients in Chengdu city, Sichuan, China

Tian

Zhang

et al. 2009

J Microbiol.

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Two hundred and seventy-three Haemophilus influenzae strains isolated from pediatric pneumonia patients in China were studied. We used Multilocus Sequence Typing (MLST) to analyze genotypic characteristics. All strains were biotyped and serotyped. Relatedness and patterns of genes among isolates were determined by the analysis of MLST and eBURST. H. influenzae primarily causes acute pneumonia in children under 1 year old. Nontypeable H. influenzae was responsible for most cases of pediatric pneumonia. All 273 strains were classified into eight biotypes. They mostly belonged to the I, II, and III biotypes (17.6%, 43.6%, and 22.7%, respectively). 62 strains (22.7%) produced beta-lactamase. We found 28 novel alleles. Fifty different STs were found by MLST, of which 39 were novel. These were ST477 through ST508 and ST521 through ST527. Group 17 and predicted founders 503 were new groups in this study. No STs correlated with strains from Korea, which is adjacent to China. The H. influenzae strains from China appeared to have heterogeneous ST types patterns which may be the reason no outbreaks or epidemics of H. influenzae infections have occurred in Chengdu city, Sichuan, China.

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Section: J Microbiolsupporting

confidence: 88%

Genotypic characteristics of Haemophilus influenzae isolates from pediatric pneumonia patients in Chengdu city, Sichuan, China

Tian

Zhang

et al. 2009

J Microbiol.

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“…In recent years, with the extensive use of antibiotics, the drug resistance of H. in uenzae strains to ampicillin has gradually increased. The ampicillin resistance rate of H. in uenzae strains in China was increased from12% in 2000-2002 [14] to 58.1% in 2016 [15]. In this study, the ampicillin resistance rate was 39.1%, which was higher than that in genital strains (26.4%) but lower than that in respiratory strains (58.4%)in 2015 reported by our study team [15].…”

Section: Discussioncontrasting

confidence: 48%

The prevalence and antimicrobial resistance of Haemophilus influenzae in prepubertal girls with vulvovaginitis

Zhou

Sun

Chen³

et al. 2020

Preprint

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Background: To determine the prevalence of Haemophilus influenzae vulvovaginitis in prepubertal girls and the antimicrobial resistance of H.influenzae strains isolated from vulval specimens.Methods: Isolates of H.influenzae from vulval swabs of prepubertal girls with vulvovaginitis received at The Children's Hospital, Zhejiang University School of Medicine during 2016-2019 were studied. Vulval specimens were inoculated on Haemophilus selective chocolate agar. Antimicrobial susceptibility tests were performed using the disk diffusion method. A cefinase disk was used to detect β-lactamase. Results: A total of 4142 vulval specimens were received during the 4 years, 649 isolates of H. influenzae were isolated from 642 girls aged 6 months to 13 years, with a median of 5y. There were peaks of isolates from April to July seen in the vulval isolates. In total, the ampicillin resistance rate was 39.1% (250/640); 33.2% strains (211/636) were for β-lactamase-positive isolates, 6.6% strains (42/635) were β-lactamase-negative and ampicillin-resistant (BLNAR) isolates. The resistance rates of H. influenzae isolates to amoxycillin-clavulanic acid, ampicillin-sulbactam, cefuroxime, ceftriaxone, cefotaxime, meropenem, levofloxacin, sulfamethoxazole-trimethoprim, azithromycin, and chloramphenicol were 26.4%, 21.8%, 24.8%, 1.7%, 1.0%, 0.2%, 0%, 47.7%, 10.2%, and 1.1%, respectively. MDR was present in 41 (6.4%) of the 642 H. influenzae isolates, with the most prevalent MDR phenotype of ampicillin-sulfamethoxazole-trimethoprim-azithromycin resistance. Conclusions: H. influenzae is a common cause of vulvovaginitis in prepubertal girls. Laboratories should ensure that they include media appropriate for the isolation of H. influenzae. It’s worth noting of ampicillin resistance of H. influenzae in clinical management.

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“…Although multidrug resistance is not as serious in Enterobacteriaceae, cefuroxime, azithromycin, and clarithromycin resistance levels are noticeably higher in ampicillin-resistant isolates than ampicillin-sensitive isolates according to our study. Before the Chinese government vigorously promoted the Hib vaccine in 2003, ampicillin-resistant isolates were uncommon, with 88.0% of isolates being susceptible to ampicillin, 100.0% being susceptible to amoxicillin/clavulanic acid, ceftriaxone, cefuroxime and azithromycin, and 99.0% being susceptible to ciprofloxacin according to a prospective muticenter study in China [ 21 ]. Most ampicillin resistance was due to beta-lactamase production.…”

Section: Discussionmentioning

confidence: 99%

“…Similar to our previous study in 2018 [ 5 ], resistance in fluoroquinolone was 3.75%, with chloramphenicol, fluoroquinolone and the third-generation cephalosporin remaining sensitive to H. influenzae . However, resistance in ampicillin has significantly increased in China compared with two decades before [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology and Antibiotic Resistance Analysis of Non-Typeable Haemophilus influenzae (NTHi) in Guangzhou: A Representative City of Southern China

et al. 2023

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This study aimed to investigate the molecular epidemiology and antibiotic resistance of Haemophilus influenzae in Guangzhou, China. A total of 80 H. influenzae isolates were collected from the First Affiliated Hospital of Guangzhou Medical University from January 2020 to April 2021. Species identification, antimicrobial susceptibility, molecular capsular typing, multilocus sequence typing and the clinical characteristics analysis of patients were performed. For all recruited isolates, the majority of H. influenzae strains from patients with respiratory symptoms were found to be non-typeable H. influenzae (NTHi). The isolates were relative susceptible to third- and fourth-generation cephalosporins, quinolones and chloramphenicol, despite having a high ampicillin resistance rate (>70%). The genotyping results reveal a total of 36 sequence types (STs), with ST12 being the most prevalent ST. Remarkably, the 36 STs identified from 80 NTHi isolates within a short period of 15 months and in a single medical setting have revealed a high genetic diversity in NTHi isolates. In comparison, it is noteworthy that the most prevalent STs found in the present study have rarely been found to overlap with those from previous studies. This is the first study on the molecular epidemiology of NTHi isolates in Guangzhou, a city that is representative of southern China.

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Resistance of Haemophilus influenzae Isolates in Children Under 5 Years Old with Acute Respiratory Infections in China between 2000 and 2002

Cited by 6 publications

References 35 publications

Genotypic characteristics of Haemophilus influenzae isolates from pediatric pneumonia patients in Chengdu city, Sichuan, China

Genotypic characteristics of Haemophilus influenzae isolates from pediatric pneumonia patients in Chengdu city, Sichuan, China

The prevalence and antimicrobial resistance of Haemophilus influenzae in prepubertal girls with vulvovaginitis

Molecular Epidemiology and Antibiotic Resistance Analysis of Non-Typeable Haemophilus influenzae (NTHi) in Guangzhou: A Representative City of Southern China

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