Resistance of Biomphalaria occidentalis from Varzea das Flores dam, Minas Gerais, to Schistosoma mansoni infection detected by low stringency polymerase chain reaction

Souza, Cecília Pereira de; Passos, Liana Konovaloff Jannotti

doi:10.1590/s0074-02762001000300017

Cited by 7 publications

(5 citation statements)

References 7 publications

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“…The understanding of the genetic basis for schistosome resistance/or susceptibility in these snail species is especially important, because schistosome eradication programs may benefit from the knowledge of the genetic polymorphism of these snails since association between hosts and parasite tend to generate and maintain genetic polymorphism (May, 1985), as has been predicted in models of schistosome-snail interactions (Morand et al, 1996). Several molecular biology techniques for studies of genetic variability in schistosomiasis vectors have been used Spatz et al, 1999;Knight et al, 1991Knight et al, , 2000Jannotti-Passos and Souza, 2000;Vidigal et al, 2000Vidigal et al, , 2001Caldeira et al, 2000Caldeira et al, , 2001Souza and Passos, 2001;Tuan and Bortolato, 2001). …”

Section: Introductionmentioning

confidence: 99%

Schistosome/mollusk: genetic compatibility

Oliveira¹,

Silva²,

Zanotti-Magalhães³

et al. 2008

Genet. Mol. Res.

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AbSTRAcT. Schistosomiasis remains one of the most prevalent parasitic infections and has significant economic and public health consequences in many developing countries. Economic development and improvement in standard of living in these countries are dependent on the elimination of this odious disease. For the control of Schistosomiasis, understanding the host/parasite association is important, since the host parasite relationship is often complex and since questions remain concerning the susceptibility of snails to infection by respective trematodes and their specificity and suitability as hosts for continued parasite development. Thus, the long term aim of this research is to learn more about the genetic basis of the snail/parasite relationship with the hope of finding novel ways to disrupt the transmission of this disease. In the current research, genetic variability among susceptible and resistant strains within and between Biomphalaria glabrata and B. tenagophila was investigated using RAPD-PCR. The results indicate great genetic variations within the two snail species using three different primers 519 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 7 (2): 518-526 (2008) Schistosome/mollusk: genetic compatibility (intrapopulational variations), while specimens from the same snail species showed few individual differences between the susceptible and resistant strains (interpopulational variation).

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Section: Introductionmentioning

confidence: 99%

Schistosome/mollusk: genetic compatibility

Oliveira¹,

Silva²,

Zanotti-Magalhães³

et al. 2008

Genet. Mol. Res.

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“…Quanto a B. intermedia, espécie de distribuição mais restrita, os dados permitiram ampliar a sua distribuição, anteriormente com ocorrências em São Paulo, Mato Grosso do Sul e Minas Gerais (PARAENSE 1975, 1985, SOUZA et al 2001.…”

Section: Discussionunclassified

“…Ao contrário dos estudos existentes sobre a biologia e a composição da fauna de peixes em reservatórios de hidrelétri-cas no Brasil (AGOSTINHO et al 2003, CARVALHO & SILVA 1999, TUNDISI et al 2002, são escassas as informações sobre os moluscos e especialmente suas interações com os parasitos (SOUZA & JANNOTTI-PASSOS 2001. Assim, de 1997 a 2004, foi efetuado um estudo qualitativo da malacofauna límnica da área de influência do lago da Usina Hidrelétrica de Serra da Mesa (UHSM), localizada no rio Tocantins, em Minaçu (13º49'26.9"S, 48º18'56.5"W); esta Usina é a maior do Brasil em volume de água represada, com 1.784 km 2 de área de superfície, atingindo oito municípios (Barro Alto, Campinaçu, Campinorte, Colinas do Sul, Minaçu, Niquelândia, Santa Rita do Novo Destino e Uruaçu).…”

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Malacofauna límnica da área de influência do lago da usina hidrelétrica de Serra da Mesa, Goiás, Brasil.: I. Estudo qualitativo

2005

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A esquistossomose é uma importante doença endêmica associada a mudanças ambientais causadas por grandes projetos econômicos, tais como a construção de usinas hidrelétricas. A dificuldade de se prever o surgimento de focos de esquistossomose associados com estes empreendimentos e a existência de condições peculiares em cada tipo de projeto requerem estudos em cada uma dessas áreas, de forma a serem implementadas medidas preventivas adequadas. O número crescente de barragens no país é preocupante pois, além de criarem condições propícias ao desenvolvimento dos moluscos vetores, aumentam a população local e o turismo. Nessas áreas, a Organização Mundial de Saúde e a Fundação Nacional de Saúde recomendam ações preventivas contra a instalação de focos de esquistossomose, dentre elas, o monitoramento das populações de moluscos. Neste trabalho são apresentados dados obtidos por nossa equipe na área da Usina Hidrelétrica de Serra da Mesa (UHSM), Goiás, onde levantamentos qualitativos e quantitativos da malacofauna límnica foram realizados de 1997 a 2004, com o principal objetivo de investigar a ocorrência e a distribuição das espécies de interesse médico e veterinário. Vinte e oito espécies, pertencentes a nove diferentes famílias foram encontradas. Além de documentar novas ocorrências no Estado, é também apresentada a distribuição de duas espécies de importância médica, Biomphalaria straminea (Dunker, 1848) e Lymnaea columella Say, 1817, bem como de duas espécies exóticas, Corbicula fluminea (Müller, 1774) e Melanoides tuberculatus (Müller, 1774).

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“…In the mid 1990's, lowstringency polymerase chain reaction (LS-PCR) was used for the identification of species of Biomphalaria Preston, 1910 (Vidigal et al, 1996;Pires et al, 1997) and for the detection of S. mansoni infection in B. glabrata (Jannotti-Passos et al, 1997), B. straminea (Dunker, 1848) and B. tenagophila (Jannotti-Passos & Souza, 2000. LS-PCR was also employed to demonstrate the resistance of B. occidentalis Paraense, 1981 against infection by S. mansoni (Souza & Jannotti-Passos, 2001).…”

Section: Amplification Of Nonspecific Dna Regionsmentioning

confidence: 99%

Molecular studies in Brazilian malacology: Tools, trends and perspectives

2013

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The use of molecular tools in Brazilian malacological studies was analyzed. We searched for these molecular tools by screening the annals of malacological events in Brazil and Latin America within the last 15 years. Information was obtained from a Brazilian curricula data bank (curriculum Lattes), which enabled the identification of the main national research groups, their main researchers and research focuses. An estimate of the scientific production on the basis of the analysis of these curricula is also provided. The main techniques and molecular markers employed were indicated. The data showed that these studies were concentrated in institutions in the South and Southeast regions and emphasized gastropods of medical importance. However, molecular studies that examined other gastropods, cephalopods and bivalves were also performed. No research study was found in the Central-West region of the country. The growth of this field of study in the country is expected, considering the molecular studies that have been performed and the great diversity of the molluscan fauna in Brazil. Future molecular studies on some taxa in the country, which have not yet been studied, should be contemplated. The increase in the use of sequencing techniques will help to bridge the gaps in our taxonomic and phylogenetic knowledge, contributing to the studies on biodiversity and conservation. Keywords: Molecular biology, malacology, snail, Brazil, DNA.

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Resistance of Biomphalaria occidentalis from Varzea das Flores dam, Minas Gerais, to Schistosoma mansoni infection detected by low stringency polymerase chain reaction

Cited by 7 publications

References 7 publications

Schistosome/mollusk: genetic compatibility

Schistosome/mollusk: genetic compatibility

Malacofauna límnica da área de influência do lago da usina hidrelétrica de Serra da Mesa, Goiás, Brasil.: I. Estudo qualitativo

Molecular studies in Brazilian malacology: Tools, trends and perspectives

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