Resistance monitoring and enzyme activity in three field populations of cowpea aphid ( Aphis craccivora ) from Egypt

Fouad, Eman; Abou-Yousef, H. M.; Abdallah, Ibrahim; Kandil, Mohammed A.

doi:10.1016/j.cropro.2015.12.015

Cited by 24 publications

(15 citation statements)

References 15 publications

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“…The greater activity of detoxifying enzymes, particularly carboxylesterase has a significant role in endowing resistance to thiamethoxamin in the cowpea aphid (Abdallah et al 2016). These results agree with Fouad et al (2016) who found that esterase activity in three field populations of cowpea aphid was higher than in the susceptible strain. The activity ratios ranged from 4.3 to 7.8-fold.…”

Section: Discussionsupporting

confidence: 83%

Role of metabolic enzymes in resistance to chlorpyrifos-methyl in the cowpea aphid, Aphis craccivora (Koch)

Mokbel¹,

Swelam²,

Radwan³

et al. 2017

Journal of Plant Protection Research

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The cowpea aphid, Aphis craccivora management relies mainly on chemical control. As a result extensive and repeated treatment of insecticides has led to the development of aphid resistance to commonly used insecticides. To investigate chlorpyrifos-methyl resistance in A. craccivora, a field strain was selected for 24-generations to achieve a resistance factor of 82.3 fold compared with a susceptible strain. In the resistant strain, malathion and lambda-cyhalothrin exhibited obvious cross-resistance; while fenvalerate and dinotefuran showed moderate cross-resistance. In contrast, slight or no cross-resistance was obtained with the other tested insecticides. To investigate metabolic resistance mechanisms, integration of biochemical and synergism assays was conducted. Results showed the key role of esterase (EST) and mixed function oxidases (MFO); however, glutathione-s-transferase (GST) contributed less to resistance. Cross-resistance studies showed the need for rotation with non-cross resistant insecticides as a resistance management tactic.

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Section: Discussionsupporting

confidence: 83%

Role of metabolic enzymes in resistance to chlorpyrifos-methyl in the cowpea aphid, Aphis craccivora (Koch)

Mokbel¹,

Swelam²,

Radwan³

et al. 2017

Journal of Plant Protection Research

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“…The M. persicae field population resistant to methomyl and omethoate exhibited a higher carboxylesterases activity compared to the laboratory susceptible strain ( Tang et al, 2017 ). Three populations of the cowpea aphid ( Aphis craccivora ) collected in Egypt showed higher carboxylesterases which may cause the resistance of the species to organophosphates, carbamates, and neonicotinoids ( Fouad et al, 2016 ). CarE enzyme activity were positively correlated with resistance level to chlorpyrifos, deltamethrin, and methomyl in the field populations of Sitobion avenae collect in wheat fields ( Zhang et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis of a Carboxylesterase Gene Associated With Isoprocarb and Cyhalothrin Resistance in Rhopalosiphum padi (L.)

Wang

Huang

et al. 2018

Front. Physiol.

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Carboxylesterase (CarE) is an important class of detoxification enzymes involved in insecticide resistance. However, the molecular mechanism of CarE-mediated insecticide resistance in Rhopalosiphum padi, a problematic agricultural pest, remains largely unknown. In the present study, an isoprocarb-resistant (IS-R) strain and a cyhalothrin-resistant (CY-R) strain were successively selected from a susceptible (SS) strain of R. padi. The enzyme activity indicated that enhanced carboxylesterase activity contributes to isoprocarb and cyhalothrin resistance. The expression levels of putative CarE genes were examined and compared among IS-R, CY-R, and SS strains, and only the R. padi carboxylesterase gene (RpCarE) was significantly over expressed in both the IS-R and CY-R strains compared to the SS strain. The coding region of the RpCarE gene was cloned and expressed in Escherichia coli. The purified RpCarE protein was able to catalyze the model substrate, α-naphtyl acetate (Kcat = 5.50 s-1; Km = 42.98 μM). HPLC assay showed that the recombinant protein had hydrolase activity against isoprocarb and cyhalothrin. The modeling and docking analyses consistently indicated these two insecticide molecules fit snugly into the catalytic pocket of RpCarE. Taken together, these findings suggest that RpCarE plays an important role in metabolic resistance to carbamates and pyrethroids in R. padi.

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“…In addition, A. craccivora can transmit two major plant viruses, bean leaf roll virus and faba bean necrotic yellows virus, which seriously affects the yield and quality of legumes (Li et al, 2013a). Insecticides, including pyrethroids, organophosphates, and neonicotinoids, are currently the primary management tool or controlling A. craccivora (Zhang et al, 2015;Fouad et al, 2016). However, due to the extensive and recurrent use of these insecticides, field populations of A. craccivora have developed insecticide resistance (Li et al, 2013a;Abd-Ella, 2014).…”

Section: Introductionmentioning

confidence: 99%

Function Analysis of P450 and GST Genes to Imidacloprid in Aphis craccivora (Koch)

Yang

Lin

et al. 2021

Front. Physiol.

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Aphis craccivora (Koch) is an economically important pest that affects legumes in worldwide. Chemical control is still the primary efficient method for A. craccivora management. However, the mechanism underlying insecticide resistance in A. craccivora has not been elucidated. A previous study observed that piperonyl butoxide (PBO) and diethyl maleate (DEM) significantly synergized imidacloprid in A. craccivora field populations, indicating that cytochrome P450 (P450) and glutathione S-transferase (GST) genes may play pivotal roles in imidacloprid resistance. In this study, 38 P450 genes and 10 GST genes were identified in A. craccivora through transcriptomic analysis. The expression levels of these P450 and GST genes were measured in susceptible (SUS) strains of A. craccivora under imidacloprid treatment with LC15, LC50, and LC85 doses. The expression levels of CYP18A1, CYP6CY21, CYP6DA1, CYP6DA2, CYP4CJ1, CYP4CJ2, and CYP380C6 were up-regulated in the three treatments. Most of these genes belong to CYP3 and CYP4 Clans. In addition, the expression levels of all P450 and GST genes in A. craccivora were also measured in the Juye (JY) and Linqing (LQ) field populations. The expression levels of CYP6DA2, CYP4CJ1, and CYP380C6 were up-regulated in the SUS strain after imidacloprid treatment at three doses, and these genes were overexpressed in the JY population. Furthermore, the sensitivity of A. craccivora to imidacloprid was significantly increased after knockdown of CYP380C6 and CYP6DA2 through RNA interference. These results may help to elucidate the mechanisms underlying of imidacloprid resistance in A. craccivora.

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Resistance monitoring and enzyme activity in three field populations of cowpea aphid ( Aphis craccivora ) from Egypt

Cited by 24 publications

References 15 publications

Role of metabolic enzymes in resistance to chlorpyrifos-methyl in the cowpea aphid, Aphis craccivora (Koch)

Role of metabolic enzymes in resistance to chlorpyrifos-methyl in the cowpea aphid, Aphis craccivora (Koch)

Functional Analysis of a Carboxylesterase Gene Associated With Isoprocarb and Cyhalothrin Resistance in Rhopalosiphum padi (L.)

Function Analysis of P450 and GST Genes to Imidacloprid in Aphis craccivora (Koch)

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