Resistance mechanisms and remediation potential of hexavalent chromium in Pseudomonas sp. strain AN-B15

Shi, Yu; Wang, Zitong; Li, Huifen; Yan, Zhengjian; Meng, Zhuang; Liu, Chang-e; Chen, Jinquan; Duan, Changqun

doi:10.1016/j.ecoenv.2023.114498

Cited by 11 publications

(2 citation statements)

References 42 publications

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“…Recently, we identi ed a plant-associated strain, i.e., Pseudomonas sp., AN-B15, which e ciently reduced Cr(VI) to soluble organo-Cr(III) in solution, which is a typical end product in the genus Pseudomonas for biotic Cr(VI) reduction (Shi et al, 2023). We hypothesized that biochar can facilitate Cr(VI)-reducing bacteria for soil remediation by directly reducing Cr(VI), thus improving soil biochemical properties and changing the soil bacterial community structure.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms involving the facilitation of the remediation of Cr(VI) contaminated soil by bacteria-loaded biochar

Huang,

Luo,

Shi

et al. 2024

Preprint

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The combination of biochar and bacteria can effectively remediate Cr(VI)-contaminated soils. However, the synergistic mechanisms remain unclear and require further investigation. In this study, we investigate the effects of a combination of biochar and Pseudomonas sp. The effects of AN-B15 on Cr(VI) transformation, soil biochemical properties, and soil microbiome are investigated, and the results show that biochar facilitated bacterial Cr(VI) removal from a solution. Specifically, the Cr(VI) removal rates by biochar are 54.44% and 42.78%, which show improvements of 14% and 20.58% by the combination of biochar and strain AN-B15 in solutions with 50 and 100 mg/L Cr(VI), respectively. Moreover, soil incubation experiments show that the bacteria-loaded biochar remediated Cr (VI)-contaminated soil more effectively than biochar or strain AN-B15 alone. Alkaline digestion shows that the amendment of 5% bacteria-loaded biochar facilitates 33.5% total soil Cr(VI) removal compared with treatment without any addition. In general, the synergistic mechanisms can be summarized as either direct or indirect immobilization. Regarding direct reduction, biochar immobilizes Cr(VI) by adsorption and abiotically reduction, which protects bacteria under the stress of Cr(VI) and enhances bacterial Cr(VI) reduction to soluble organo-Cr(III). Regarding indirect immobilization, bacteria-loaded biochar improves the soil biochemical properties, which are beneficial to Cr(VI) reduction. Moreover, bacteria-loaded biochar immobilized Cr(VI) by impacting soil bacterial communities, which increases the relative abundance of indigenous helpful bacteria to improve soil quality and reduce Cr(VI). Notably, Nocardioides is a key genus in the decline of Cr(VI) into organically integrated Cr in soil. This study deepens our understanding of the transformation of soil Cr(VI) by bacteria-loaded biochar and can contribute to the optimization of Cr(VI) remediation strategies.

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Section: Introductionmentioning

confidence: 99%

Mechanisms involving the facilitation of the remediation of Cr(VI) contaminated soil by bacteria-loaded biochar

Huang,

Luo,

Shi

et al. 2024

Preprint

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show abstract

“…21 Several practices for using native microbes for remediation of heavy metal-contaminated soils have been proposed. For example, according to Shi et al 22 inoculating strain AN-B15 increased the fresh weight of wheat roots and shoots by 55.5% and 18.8%, respectively under Cr( vi ) stress (5 mg L −1 ) and reduced Cr induced toxicity and plant uptake of Cr, indicating its potential for safe cultivation in Cr( vi ) polluted soil. Ali et al 23 discovered that the application of citric acid increased plant growth even at a high concentration of Cr; however, the application of citric acid with S. aureus inoculum further boosted the plant growth and promoted the absorption of Cr 3+ and Cr 6+ , enhancing the phytoremediation potential of castor bean.…”

Section: Introductionmentioning

confidence: 99%