Resistance development in Cotton mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) to insecticides from Organophosphate, Thiadiazines and Thiourea derivatives

Nagrare, V. S.; Fand, Babasaheb B.; Naik, V. Chinna Babu; Naikwadi, Bhausaheb; Deshmukh, Vrushali; Sinh, Dharmendra

doi:10.1007/s42690-019-00068-9

Cited by 16 publications

(12 citation statements)

References 28 publications

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“…Paracoccus marginatus were provided by the Environment and Plant Research Institution, Chinese Academy of Tropical Agriculture Sciences. Mealybugs were maintained on potato spouts ( Potato tuberosum L.) supported with soil in plastic containers as a growing media ( Nagrare et al 2019 ). The insects were kept at 25 ± 1°C and 60–70% relative humidity (RH) under a 14 L: 10 D photoperiod in artificial climate chambers.…”

Section: Methodsmentioning

confidence: 99%

Reference Gene Selection for Analyzing the Transcription Patterns of Two Fatty Acyl-CoA Reductase Genes From Paracoccus marginatus (Hemiptera: Pseudococcidae)

Liang

Chen

et al. 2021

Journal of Insect Science

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Paracoccus marginatus (Hemiptera: Pseudococcidae), known as the papaya mealybug, could cause considerable yield loss of several plants. To date, there is no molecular-based study of P. marginatus. Fatty acyl-CoA reductases (FARs) are key enzymes involved in wax synthesis. In the present study, we cloned and characterized coding sequences (CDS) of two FAR genes from P. marginatus. The results showed that PmFAR1 and PmFAR2 CDS were 1,590 and 1,497 bp in length, respectively, and sequence analysis indicated that these two genes both had the conservative motifs belonging to FAR_C superfamily. Furthermore, seven candidate reference genes were analyzed for their expression stability by using common algorithms including comparative ΔCq method, geNorm, NormFinder, BestKeeper, and RefFinder. Eventually, β-actin and GAPDH were the best reference genes in evaluating the expression of those two FAR genes. We found that PmFAR1 and PmFAR2 showed distinct expression patterns in different life stages. Moreover, the transcription of PmFAR1 and PmFAR2 in P. marginatus fed on resistant cassava cultivars was significantly lower compared with those fed on susceptible ones, indicating the potential function of FAR genes in cassava resistance to P. marginatus. The present study might help in better understanding the molecular mechanism of cassava resistance to mealybug.

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Section: Methodsmentioning

confidence: 99%

Reference Gene Selection for Analyzing the Transcription Patterns of Two Fatty Acyl-CoA Reductase Genes From Paracoccus marginatus (Hemiptera: Pseudococcidae)

Liang

Chen

et al. 2021

Journal of Insect Science

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“…133 Insecticide resistance in P. solenopsis is a challenging pest management issue with consequences including crop losses, increased environmental pollution, risks of applicator and agricultural worker exposure from higher rates of pesticide application, wastage of resources, increased production costs, socio-economic problems and, in the most extreme case, the complete destruction of agricultural production systems on a local or regional basis. 134,135 Insecticide resistance in P. solenopsis was first reported by Saddiq et al 136 137 in Maharashtra, India in 2020. During initial invasion in 2005, P. solenopsis was highly susceptible to a wide range of insecticides, yet after a few years, it had developed resistance to organophosphates, pyrethroids and neonicotinoids from low to high levels in nymphal instars, although resistance of the adult stage remains unknown.…”

Section: Development Of Insecticide Resistancementioning

confidence: 99%

“…The P. solenopsis population from Maharashtra (19.7515°N, 75.7139°E) had a higher level of resistance against buprofezin and a lower resistance level against organophosphates and diafenthiuron than that of the P. solenopsis population from Amravati (20.9320°N, 77.7523°E) city. 137 Saddiq et al 136 reported the various levels of resistance in P. solenopsis in response to organophosphate and pyrethroids. Insecticide-susceptible P. solenopsis strains were used to evaluate the district-wide patterns of organophosphate and pyrethroid resistance by testing field populations in the cotton-growing areas of southern Punjab.…”

Section: Development Of Insecticide Resistancementioning

confidence: 99%

Biology, ecology, and management of cotton mealybug Phenacoccus solenopsisTinsley (Hemiptera: Pseudococcidae)

Waqas

Shi

et al. 2021

Pest Management Science

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The cotton mealybug Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae), is a highly invasive and harmful pest. It causes considerable loss of cotton crops in China, India and Pakistan. Little is known about its bionomics since it was first recorded in Pakistan and India in 2005. Rapid spread of this pest worldwide has accelerated research on its biology, ecology and management. The P. solenopsis has a short life cycle, and optimal temperatures lead to an increase in the number of generations per year, which is a serious threat to cotton crop production. Cotton mealybug is native to the USA, although it has now spread to >43 countries. Insecticidal control is the primary and dominant practice for this pest, and its resistance to commonly used insecticides is increasing. Biocontrol agents have strong potential for the management of nymphal instar stages. We read >250 articles related to our review title and finally reviewed recent advances in the understanding of P. solenopsis biology, ecology and control approaches, aiming to highlight integrated and biological management practices of this pest.

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“…However, the indiscriminate and recurrent use of pesticides against the mealybugs is unsustainable due to environmental degradation, biodiversity loss, and resistance development ( Fouad et al., 2016 ). However, studies have reported mealybugs with the resistance development to many well-known classes of pesticides, including avermectins, carbohydrazide, cyclodiene, IGRs, neonicotinoids, organophosphates, oxadiazine, and pyrethroids in recent times ( Saddiq et al., 2014 ; Nagrare et al., 2020 ). Owing to the adverse impact of the chemical pesticides on the public and the environment, an immediate replacement is required to successfully control the mealybugs.…”

Section: Introductionmentioning

confidence: 99%

Toxicity and synergistic activity of compounds from essential oils and their effect on detoxification enzymes against Planococcus lilacinus

2022

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Mealybug, Planococcus lilacinus Cockerell, is a primary surface-feeding insect pest of fruit and flowering plants and also transmits plant viruses, resulting in economic crop loss. Inappropriate and recurrent use of pesticides for mealybug control results in resistance building and deleterious effects on humans and the environment. Essential oils are the most excellent choice for insecticides. Insecticidal activities of pure compounds of essential oils against P. lilacinus are not reported. The present study aims to study the insecticidal activities of some pure active compounds and their binary mixture’s action by topical application against P. lilacinus. Results showed that the pure compounds of L-limonene, β-myrcene, and ocimene revealed toxicity (each at LD50 = 0.37 µg/insect) after 96 h. The binary mixtures of geraniol + L-menthol and L-limonene + geraniol exhibited synergistic effects (each at LD50 = 0.03 µg/insect) after 96 h. The monoterpenes of ocimene and β-myrcene at the higher concentration of 5,000 ppm substantially inhibited the detoxification enzyme activities of AChE (0.93 and 0.78 mU/mg, respectively) and GST (2.19 and 7.29 nmol/min/ml, respectively) in P. lilacinus after 48 h. SEM analysis reported the significant anomalies on the morphology of abdominal cuticle, setae, and thoracic leg after 96-h treatment with ocimene at 1,250 ppm against P. lilacinus. Based on the results, the tested pure compounds and their combinations can be suggested for the control of mealybugs.

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Resistance development in Cotton mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) to insecticides from Organophosphate, Thiadiazines and Thiourea derivatives

Cited by 16 publications

References 28 publications

Reference Gene Selection for Analyzing the Transcription Patterns of Two Fatty Acyl-CoA Reductase Genes From Paracoccus marginatus (Hemiptera: Pseudococcidae)

Reference Gene Selection for Analyzing the Transcription Patterns of Two Fatty Acyl-CoA Reductase Genes From Paracoccus marginatus (Hemiptera: Pseudococcidae)

Biology, ecology, and management of cotton mealybug Phenacoccus solenopsisTinsley (Hemiptera: Pseudococcidae)

Toxicity and synergistic activity of compounds from essential oils and their effect on detoxification enzymes against Planococcus lilacinus

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