Resistance and virulence determinants of faecal Salmonella spp. isolated from slaughter animals in Benin

Abstract

Objective Salmonella spp. are one of the leading foodborne pathogens worldwide naturally found in the intestines of many animals. People that are in direct contact with the infected animals or their cages may become ill. The aim of this study was to determine the prevalence, antibiogram and virulence genes associated with Salmonella serovars from fecal samples of animals intended for consumption in Southern Benin. Results Out of a total of 406 samples, 2.46% were positive. The isolates identified were multidrug-resistant Salmonella spp. to penicillins, first generation cephalosporins and some aminoglycosides. All Salmonella isolates produced inv A gene of 284 bp, fim A of 85 bp and stn of 260 bp. The spvC gene (571 bp) was present in 10% of the isolates whereas the spvR gene (310 bp) was found in 20% of the isolates. The control strain possessed all the tested genes. The invA gene implies that strains are able to invade epithelial cells. The fimA and stn genes present in all isolates show that they are capable of causing gastrointestinal illness in humans. The presence of spvC and spvR genes suggests the possibility of these strains to produce toxins. Electronic supplementary material The online version of this article (10.1186/s13104-019-4341-x) contains supplementary material, which is available to authorized users.

Evaluation of in-vivo anti-Salmonella activity of Uvaria chamae, Lantana camara and Phyllantus amarus used in Benin, West Africa

“…Table 9 Evolution of cloacal aspect from three-day-old chicks inoculated with Salmonella Typhimurium and treated with Leaves aqueous extract of U. chamae and Colistin Fig. 3 weight changes of chicks orally infected with Salmonella Typhimurium 14028 and treated with leaves aqueous extract of U. chamae and Colistin Table 10 Evolution of cloacal aspect from three-day-old chicks inoculated with P19 Salmonella and treated with Leaves aqueous extract of U. Chamae and Colistin -Ten multiresistant strains of Salmonella spp: they were isolated by Deguenon et al [25]. The strains were multidrug-resistant to penicillins, first generation cephalosporins and some aminoglycosides.…”

“…For example, Pande et al [24] used with success henses of 14 weeks for oral induction with S. Typhimurium PT 9. The virulence of Salmonella Typhimurium ATCC 14028 was a guarantee because these virulence factors have been characterized by PCR by Deguenon et al [25]. This study showed that the strain has at least 5 virulence genes: invA, spvR, SpvC, FimA and Stn.…”

“…Presence of fimA gene indicates the presence of fimbriae which is important for Salmonella spp. to adhere to epithelial cells [25]. Stn gene is suspected to contribute to enterotoxigenic potency [28].…”

“…A preliminary microbiological examination of the cloaca of the chicks helped to check if chicks are exempt from Salmonella spp. A cloaca swab was performed on all chicks and Salmonella spp were searched according to the method described by Deguenon et al [25].…”

“…On days 3, 6, and 9, the feces from each lots of chicks were collected. Salmonella was sought using method described par Deguenon et al [25]. Five (5) grams of fecal samples were transferred immediately following collection in 45 ml of buffered peptone water.…”