Resina Draconis Reduces Acute Liver Injury and Promotes Liver Regeneration after 2/3 Partial Hepatectomy in Mice

Abstract

Aim. To investigate the protective effects and possible mechanisms of action of resina draconis (RD) on acute liver injury and liver regeneration after 2/3 partial hepatectomy (PH) in mice. Methods. 2/3 PH was used to induce acute liver injury. Mice were divided into three groups: sham, vehicle + 2/3 PH, and RD + 2/3 PH. Resina draconis was administered intragastrically after 2/3 PH into the RD + 2/3 PH group, and the same volume of vehicle (1% sodium carboxymethyl cellulose) was injected into the vehicle + 2/3 PH group and sham group mice. The index of liver to body weight (ILBW) and proliferating cell nuclear antigen (PCNA) were assayed to evaluate liver regeneration. Blood and liver tissues were collected for serological and western blotting analysis. Results. Resina draconis protected against 2/3 PH-induced acute severe liver injury and promoted liver regeneration as shown by significantly increased ILBW compared with that of controls. 2/3 PH increased serum AST and ALT levels, which were significantly decreased by RD treatment, while 2/3 PH decreased serum TP and ALB, which were increased by RD treatment. In the RD + 2/3 PH group, PCNA expression was significantly increased compared with the 2/3 PH group. Further, hepatocyte growth factor (HGF), TNFα, and EGFR levels were increased in the RD group at postoperative days 2 and 4 compared with the those in the 2/3 PH group. Conclusion. Our results suggest that RD ameliorates acute hepatic injury and promotes liver cell proliferation, liver weight restoration, and liver function after 2/3 PH, probably via HGF, TNFα, and EGFR signaling.

WITHDRAWN: Investigation of regenerative effects of Theranekron ® and Misoprostol after partial hepatectomy in rats

“…ALT is more liver speci c to determine hepatocellular damage 31 . It is reported that these enzymes were increased during partial hepatectomy [32][33][34] . In our study, although there was an increase in ALT, AST, ALP and GGT enzymes in the hepatectomy group compared to the control group, but there was not any statistically signi cant.…”

Investigation of Regenerative Effects of Theranekron® and Misoprostol after Partial Hepatectomy in Rats

“…ALT is more liver specific to determine hepatocellular damage [31]. It is reported that these enzymes were increased during partial hepatectomy [32][33][34]. In our study, although there was an increase in ALT, AST, ALP and GGT enzymes in the hepatectomy group compared to the control group, but there was not any statistically significant.…”

Signaling pathways of liver regeneration: Biological mechanisms and implications

“…For instance, studies have shown that activating signaling pathways such as HGF, EGFR, and TNF-α prevents acute liver failure and promotes LR. 225 In PHx after cirrhosis, the IL-6 signaling pathway can maintain compensatory LR by enhancing STAT3 activation, 226 the continuous activation of IL-6 signaling pathway can lead to the development of HCC. In mice with carbon tetrachloride (CCl4),-induced cirrhosis, the TGF-β receptor type I(TGFβR1) kinase inhibitor galunisertib (LY2157299) can block the liver fibrosis process and promote LR by inhibiting the TGF-β signaling pathway.…”