Resin-based micropipette tip for immunochromatographic assays in urine samples

Yuhi, Teruko; Nagatani, Naoki; Endo, Tatsuro; Kerman, Kağan; Takata, Masayuki; Konaka, Hiroyuki; Namiki, Mikio; Takamura, Yuzuru; Tamiya, Eiichi

doi:10.1016/j.jim.2006.02.011

Cited by 25 publications

(23 citation statements)

References 18 publications

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“…This is because when the positive serum was diluted to 1/50, the test line of the larger sized group (upper picture) shows a little red color but the smaller sized group (lower picture) does not. This result is coincident to Teruko Yuhi's work 26 and another literature survey. 27 …”

Section: Comparison Of the Real Sample Testsupporting

confidence: 88%

A gold nanoparticle-based immunochromatographic assay: The influence of nanoparticulate size

Lou

et al. 2012

Analyst

101

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Four different sized gold nanoparticles (14 nm, 16 nm, 35 nm and 38 nm) were prepared to conjugate an antibody for a gold nanoparticle-based immunochromatographic assay which has many applications in both basic research and clinical diagnosis. This study focuses on the conjugation efficiency of the antibody with different sized gold nanoparticles. The effect of factors such as pH value and concentration of antibody has been quantificationally discussed using spectra methods after adding 1 wt% NaCl which induced gold nanoparticle aggregation. It was found that different sized gold nanoparticles had different conjugation efficiencies under different pH values and concentrations of antibody. Among the four sized gold nanoparticles, the 16 nm gold nanoparticles have the minimum requirement for antibody concentrations to avoid aggregation comparing to other sized gold nanoparticles but are less sensitive for detecting the real sample compared to the 38 nm gold nanoparticles. Consequently, different sized gold nanoparticles should be labeled with antibody under optimal pH value and optimal concentrations of antibody. It will be helpful for the application of antibody-labeled gold nanoparticles in the fields of clinic diagnosis, environmental analysis and so on in future.

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Section: Comparison Of the Real Sample Testsupporting

confidence: 88%

A gold nanoparticle-based immunochromatographic assay: The influence of nanoparticulate size

Lou

et al. 2012

Analyst

101

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“…The lower detection sensitivity in IgG/HSA-depleted samples compared to IgG-depleted samples could in part be due to the role that HSA plays in blocking non-specific adsorption. 20 The absence of HSA (depleted successfully to < 3% 2 and in this study), could have increased non-specific adsorption, thus reducing the potential "optimal" sensitivity of the assay. This highlights the need for detailed studies on high-abundant protein depletion and their individual impact on downstream assays and their applications.…”

Section: Application Of Depletion Protocol To Detection Of Prostate Smentioning

confidence: 76%

A Rapid Sample Pretreatment Protocol: Improved Sensitivity in the Detection of a Low-abundant Serum Biomarker for Prostate Cancer

Vestergaard

Tamiya

2007

ANAL. SCI.

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The detection of small and low abundant biomarkers in clinical samples remains a challenge, mainly due to the presence of high-abundant proteins, such as albumin (HSA) and immunoglobulin G (IgG), accounting for at least 65% of plasma proteins. 1 The depletion of these proteins could facilitate biomarker discovery and detection. Depletion should aim to provide the selective removal of high-abundant proteins, and concentrate components of low abundance. Commercially available methods for IgG and HSA depletion have enabled the visualization of new protein signals, the identification of gene products and improved detection of low-abundant proteins. [2][3][4] Most of the methods utilize either antibody-or dye-based resins, 3,5 molecular size filtration, organic solvents, and ammonium sulfate precipitation, as well as reverse phase and ion exchange chromatography. 5,6 Also available are resins cross-linked with proteins A and G, and their recombinants for IgG removal. 3,7 Antibody-based resins display higher specificity and efficiency, 4,8 but are more expensive than the dye-based resins, limiting their usage. Commercially available pre-packed columns lack the flexibility to accommodate issues pertaining to sample size, for example. In addition, some rely on use of pumps and other equipment to operate smoothly, 2,3 thus limiting their use in many health centers. Most studies on IgG/HSA depletion have focused on the detection of lowabundant proteins using electrophoresis and mass spectrometry.2,3,9 As far as we are aware, the effects of depletion on point-of-care-testing (POCT) immunochromatography membrane-based assays is yet to be reported. Further, an evaluation of the potential benefits of HSA depletion over IgG, and vice versa has received very little attention to date.In this report, we present (a) a versatile method for HSA and IgG depletion from quality control (QC) and human serum samples, (b) assess the effect of depletion on human chorionic gonadotropin (HCG) and prostate specific antigen (PSA) using immunochromatography test strips (ICTS), and (c) evaluate the effects of HSA depletion compared to that of IgG on HCG and PSA detection. We employed a dye-based removal of HSA using Cibacron Blue 3G-A Sepharose (SB). SB has a strong affinity towards HSA. 10 For the removal of IgG, we exploited the high affinity between the Fc region of antibodies and protein G using sepharose beads cross-linked with protein G (S-PG). 7 Experimental MaterialsSB and S-PG were purchased from GE Healthcare BioSciences AB (Uppsala, Sweden). Handee TM spin columns (500 μL) and IgG specific kit (Easy-Titre ® IgG assay kit) were purchased from Pierce (IL, USA). HSA blue fluorescent assay kit was from Active Motif Japan (Tokyo, Japan). Prostate specific antigen (PSA) was obtained from Chemicon International (Temecula, CA, USA). Unless otherwise stated, all other reagents were from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). All solutions were prepared and diluted using ultra-pure water (18.3 MΩ cm -1 ) from the Millipore Milli-Q ...

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“…An immunochromatographic SPR test has been reported for the determination of hCG, which is based on the immobilization of primary antibodies in a thin zone of the chromatographic support, in which the analyte is accumulated, and the conjugation of the secondary antibodies with AuNPs [63]. Other similar immunochromatographic tests for PSA and hCG determination involve the measurement of the intense red color owing to the accumulation of the AuNPs [64][65][66].…”

Section: Sandwich Assaysmentioning

confidence: 99%

“…In some instances, these NPs are only used as nanoscaffolds to improve the immobilization of the antibodies [19,20,84], although other methods involve the measurement of a property of the AuNPs, which is correlated with the analyte concentration, such as SPR [63], light scattering [48,49] and color intensity image [64][65][66]. The lowest LOD reported corresponds to a sandwich assay using this last detection system [65].…”

Section: Individual Determinationsmentioning

confidence: 99%