Resilience of planktonic and biofilm cultures to supercritical CO2

Mitchell, Andrew C.; Phillips, Adrienne J.; Hamilton, Marty A.; Gerlach, Robin; Hollis, W. Kirk; Kaszuba, John P.; Cunningham, Alfred B.

doi:10.1016/j.supflu.2008.07.005

Cited by 73 publications

(81 citation statements)

References 47 publications

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“…Survival of spores and biofilms after short-term scCO 2 exposure (i.e., minutes to hours) (8,9,15,16) is well documented, and recent studies show that mineral matrices may enhance microbial survival of scCO 2 exposure by providing substrates for biofilm formation and/or by creating buffered microenvironments (10,17). Biogeochemical models also suggest that diverse forms of microbial metabolism are thermodynamically favorable under reservoir conditions post-CO 2 injection (3,18), where an aqueous phase in direct contact with scCO 2 may have dissolved CO 2 concentrations exceeding 2.5 M (3).…”

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confidence: 99%

“…Microbial activity can influence the various trapping mechanisms that are crucial to permanent storage of sequestered CO 2 . Prior work has documented that microbial biofilms can be employed to plug pore spaces and impede the flow of scCO 2 through sandstone cores, providing a means of "structural trapping" for a mobile CO 2 phase (15,23). Trapping of CO 2 residuals in pore spaces by capillary forces (residual trapping) may be affected by biosurfactant effects on wetting (24).…”

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confidence: 99%

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Microbial Growth under Supercritical CO ₂

Peet

Freedman

Hernandez

et al. 2015

Appl Environ Microbiol

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Growth of microorganisms in environments containing CO 2 above its critical point is unexpected due to a combination of deleterious effects, including cytoplasmic acidification and membrane destabilization. Thus, supercritical CO 2 (scCO 2 ) is generally regarded as a sterilizing agent. We report isolation of bacteria from three sites targeted for geologic carbon dioxide sequestration (GCS) that are capable of growth in pressurized bioreactors containing scCO 2 . Analysis of 16S rRNA genes from scCO 2 enrichment cultures revealed microbial assemblages of varied complexity, including representatives of the genus Bacillus. Propagation of enrichment cultures under scCO 2 headspace led to isolation of six strains corresponding to Bacillus cereus, Bacillus subterraneus, Bacillus amyloliquefaciens, Bacillus safensis, and Bacillus megaterium. Isolates are spore-forming, facultative anaerobes and capable of germination and growth under an scCO 2 headspace. In addition to these isolates, several Bacillus type strains grew under scCO 2 , suggesting that this may be a shared feature of spore-forming Bacillus spp. Our results provide direct evidence of microbial activity at the interface between scCO 2 and an aqueous phase. Since microbial activity can influence the key mechanisms for permanent storage of sequestered CO 2 (i.e., structural, residual, solubility, and mineral trapping), our work suggests that during GCS microorganisms may grow and catalyze biological reactions that influence the fate and transport of CO 2 in the deep subsurface.

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confidence: 99%

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confidence: 99%

Microbial Growth under Supercritical CO ₂

Peet

Freedman

Hernandez

et al. 2015

Appl Environ Microbiol

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“…CO 2 will reside in the reservoir as a plume of supercritical fluid and will gradually dissolve into the water. Given that high aqueous CO 2 concentrations are generally toxic to most microorganisms as the molecule interferes with intracellular functions (Hong and Pyun, 1999;Spilimbergo and Bertucco, 2003;Watanabe et al, 2003;Damar and Balaban, 2006;Mitchell et al, 2008;Santillan et al, 2013), this change will initially stress the native microbial population. However, over longer time scales, these new conditions may create a niche for CO 2 -tolerant bacteria that will select for a new community of microorganisms capable of resisting CO 2 toxicity.…”

Section: Introductionmentioning

confidence: 99%

“…This in turn, can complement existing genomic and metagenomic studies which can only confirm and infer the presence and activities of microbes based on sequence data. Previous culture-based approaches have also been performed utilizing samples from environmental sites (Mitchell et al, 2008;Kirk et al, 2013;Peet et al, 2015). Our approach was to work with a field site that had been under prolonged CO 2 perturbation (Burnside et al, 2013) to find out whether microbial communities under prolonged CO 2 exposure would still be viable.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a CO2-tolerant Lactobacillus strain from Crystal Geyser, Utah, U.S.A.

et al. 2015

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When CO 2 is sequestered into the deep subsurface, changes to the subsurface microbial community will occur. Capnophiles, microorganisms that grow in CO 2 -rich environments, are some organisms that may be selected for under the new environmental conditions. To determine whether capnophiles comprise an important part of CO 2 -rich environments, an isolate from Crystal Geyser, Utah, U.S.A., a CO 2 -rich spring considered a carbon sequestration analog, was characterized. The isolate was cultured under varying CO 2 , pH, salinity, and temperature, as well as different carbon substrates and terminal electron acceptors (TEAs) to elucidate growth conditions and metabolic activity. Designated CG-1, the isolate is related (99%) to Lactobacillus casei in 16S rRNA gene identity, growing at PCO 2 between 0 and 1.0 MPa. Growth is inhibited at 2.5 MPa, but stationary phase cultures exposed to this pressure survive beyond 5 days. At 5.0 MPa, survival is at least 24 h. CG-1 grows in neutral pH, 0.25 M NaCl, and between 25 • and 45 • C and consumes glucose, lactose, sucrose, or crude oil, likely performing lactic acid fermentation. Fatty acid profiles between 0.1 and 1.0 MPa suggests decreases in cell size and increases in membrane rigidity. Transmission electron microscopy reveals rod shaped bacteria at 0.1 MPa. At 1.0 MPa, cells are smaller, amorphous, and produce abundant capsular material. Its ability to grow in environments regardless of the presence of CO 2 suggests we have isolated an organism that is more capnotolerant than capnophilic. Results also show that microorganisms are capable of surviving the stressful conditions created by the introduction of CO 2 for sequestration. Furthermore, our ability to culture an environmental isolate indicates that organisms found in CO 2 environments from previous genomic and metagenomics studies are viable, metabolizing, and potentially affecting the surrounding environment.

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“…This is referred to as osmotic swelling and deswelling, respectively, and is handled as an additional pressure. Osmotic swelling has been observed in biofilms (Mitchell et al, 2008;Hentzer et al, 2001) as well as in agarose hydrogel models of EPS (Strathmann et al, 2001).…”

Section: Mechanical and Chemical Forcesmentioning

confidence: 99%