Residue screening and analysis of enrofloxacin and its metabolites in real aquatic products based on ultrahigh-performance liquid chromatography coupled with high resolution mass spectrometry

Dai, Jinxia; Wang, Ye; Lin, Hui; Sun, Yanmin; Pan, Yuning; Qiao, Jun-qin; Lian, Hong-zhen; Xu, Chun-xiang

doi:10.1016/j.foodchem.2022.134757

Cited by 27 publications

(11 citation statements)

References 31 publications

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“…Recent studies have shown that enrofloxacin and ciprofloxacin residues are of significant concern [ 21 ]. Enrofloxacin is widely used due to its broad spectrum of antibacterial activity, strong bactericidal effects, and rapid action [ 39 ], making it one of the most frequently detected veterinary antibiotics in aquatic products [ 40 ]. Griboff et al, during their research on fish in the Argentine market, found enrofloxacin to be present in 100% of their samples [ 41 ].…”

Section: Resultsmentioning

confidence: 99%

“…Enrofloxacin has been banned in aquaculture in countries like the US, Canada, and Chile due to the risks it poses to consumers [ 54 , 55 ]. Nevertheless, enrofloxacin is still being employed in Chinese aquaculture [ 40 ]. Wang et al’s assessments in Zhejiang province unveiled the widespread utilization of enrofloxacin in aquaculture, encompassing its application in the cultivation of large yellow croakers [ 56 ].…”

Section: Resultsmentioning

confidence: 99%

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Health Risk Assessment of Antibiotic Pollutants in Large Yellow Croakers from Zhejiang Aquaculture Sites

Li,

Jin,

Wang

et al. 2023

Foods

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Intensive aquaculture combatting the decline of large yellow croaker populations can trigger bacterial outbreaks, resulting in extensive antibiotic use. In this study, we screened 5 aquaculture sites in the coastal areas of Zhejiang and identified 17 antibiotics in large yellow croakers using UPLC-MS/MS. The distribution and occurrence of antibiotic pollutants were different in the different tissues of large yellow croakers, being primarily dominated by quinolones. Relatively higher average residue levels of enrofloxacin and ciprofloxacin were detected in the inedible parts, specifically the gills (37.29 μg/kg). Meanwhile, relatively high average residue levels of enrofloxacin and ciprofloxacin were also found in the edible parts, particularly in the muscle (23.18 μg/kg). We observed that the residue levels detected in the swim bladder exceeded the prescribed limit for fish muscle, but there is currently no specific regulatory limit established for this particular tissue. Despite the HI values of enrofloxacin and ciprofloxacin being below 0.01, the health risks should not be disregarded. The findings of this research provide significant practical implications for assessing antibiotic contamination and enhancing the risk management of coastal regions.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Health Risk Assessment of Antibiotic Pollutants in Large Yellow Croakers from Zhejiang Aquaculture Sites

Li,

Jin,

Wang

et al. 2023

Foods

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“…High-performance liquid chromatography (HPLC) or HPLC-mass spectrometry (HPLC-MS) [ 16 ], thin-layer chromatography surface-enhanced Raman spectroscopy (TLC-SERS) [ 17 , 18 ], enzyme-linked immunosorbent assay (ELISA) [ 19 ], immunochromatographic assay [ 20 ], and other methods have been employed in the majority of prior studies to determine ENR. While these traditional detection techniques are sensitive and precise, their instrumentation and sample preparation processes are expensive, time-consuming, and complicated [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Enrofloxacin Rapid Detection in Aquatic Foods: Based on DNA Aptamer Sensor

Bao,

Wang,

Hao

et al. 2024

Foods

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Enrofloxacin (ENR) is widely used as a synthetic fluoroquinolone antibiotic for disease control in aquatic animals. ENR aptamers were screened in this study using the magnetic bead-SELEX method, and a graphene oxide fluorescent sensor was developed to detect the ENR residues in aquatic products. Firstly, ENR was conjugated to amino magnetic beads by amidation reaction, and then the aptamer sequences showing high affinity to ENR were screened step by step by using the SELEX screening method. Finally, after 10 rounds of SELEX screening, six candidate aptamers with high affinity were obtained. Among these, ENR-Apt 6 was selected based on its secondary structure features, high affinity (Kd = 35.08 nM), and high specificity to ENR. Furthermore, a fluorescent sensor was prepared using graphene oxide and ENR-Apt 6. The results showed that the linear range of the sensor could reach 600 nM (R2 = 0.986), while its optimal linear range was 1–400 nM (R2 = 0.991), with the lowest detection limit of 14.72 nM. The prepared sensor was successfully used for the detection of ENR in real samples, with a recovery range of 83.676–114.992% and a relative standard deviation < 10% for most of the samples.

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“…To date, many commercial analytical methods for norfloxacin detection have been reported, including those using enzyme immunoassay (EIA), 5 voltammetry, surface-enhanced Raman spectroscopy (SERS), 8,9 electrochemistry 10 and high-performance liquid chromatography (HPLC). 11 These methods are highly accurate for detecting norfloxacin but require expensive instrumentation, complex sample preparation steps, rigorous handling skills and sophisticated experimental conditions. 12 Therefore, there is still an urgent need for simple and effective methods for sensitive monitoring of norfloxacin.…”

Section: Introductionmentioning

confidence: 99%

Y³⁺@CdTe quantum dot nanoprobe as a fluorescence signal enhancement sensing platform for the visualization of norfloxacin

Chen

Jiang

Liu

et al. 2023

Analyst

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Residue screening and analysis of enrofloxacin and its metabolites in real aquatic products based on ultrahigh-performance liquid chromatography coupled with high resolution mass spectrometry

Cited by 27 publications

References 31 publications

Health Risk Assessment of Antibiotic Pollutants in Large Yellow Croakers from Zhejiang Aquaculture Sites

Health Risk Assessment of Antibiotic Pollutants in Large Yellow Croakers from Zhejiang Aquaculture Sites

Enrofloxacin Rapid Detection in Aquatic Foods: Based on DNA Aptamer Sensor

Y³⁺@CdTe quantum dot nanoprobe as a fluorescence signal enhancement sensing platform for the visualization of norfloxacin

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Residue screening and analysis of enrofloxacin and its metabolites in real aquatic products based on ultrahigh-performance liquid chromatography coupled with high resolution mass spectrometry

Cited by 27 publications

References 31 publications

Health Risk Assessment of Antibiotic Pollutants in Large Yellow Croakers from Zhejiang Aquaculture Sites

Health Risk Assessment of Antibiotic Pollutants in Large Yellow Croakers from Zhejiang Aquaculture Sites

Enrofloxacin Rapid Detection in Aquatic Foods: Based on DNA Aptamer Sensor

Y3+@CdTe quantum dot nanoprobe as a fluorescence signal enhancement sensing platform for the visualization of norfloxacin

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Product

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Y³⁺@CdTe quantum dot nanoprobe as a fluorescence signal enhancement sensing platform for the visualization of norfloxacin