Residue analysis of veterinary drugs and growth-promoting agents

Stolker, A.A.M.; Zuidema, T.; Nielen, Michel W. F.

doi:10.1016/j.trac.2007.09.008

Cited by 150 publications

(62 citation statements)

References 41 publications

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“…The majority of current LC-MS based hormone and veterinary drug residue analyses relies on the high sensitivity and selectivity of triple quadrupole tandem mass spectrometry (QqQ-MS/MS) systems operated in the multiple reaction-monitoring (MRM) mode. The two-stage mass selection provides the selectivity and sensitivity to enable the detection, identification, and quantification of preselected targets at low ng/g level in complex biological matrices such as urine, feces, tissue, feed, and hair [1]. The recent introduction of ultra-performance (UP)LC and fast-switching QqQ-MS/MS instruments significantly increased the number of targets that can be detected in one analysis [2].…”

mentioning

confidence: 99%

Full-scan accurate mass selectivity of ultra-performance liquid chromatography combined with time-of-flight and orbitrap mass spectrometry in hormone and veterinary drug residue analysis

Heeft¹,

Bolck²,

Beumer³

et al. 2009

J. Am. Soc. Mass Spectrom.

128

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The applicability of ultra-performance liquid chromatography (UPLC) combined with fullscan accurate mass time-of-flight (TOF) and Orbitrap mass spectrometry (MS) to the analysis of hormone and veterinary drug residues was evaluated. Extracts from blank bovine hair were fortified with 14 steroid esters. UPLC-Orbitrap MS performed at a resolving power of 60,000 (FWHM) enabled the detection and accurate mass measurement (Ͻ3 ppm error) of all 14 steroid esters at low ng/g concentration level, despite the complex matrix background. A 5 ppm mass tolerance window proved to be essential to generate highly selective reconstructed ion chromatograms (RICs) having reduced background from the hair matrix. UPLC-Orbitrap MS at a lower resolving power of 7500 and UPLC-TOFMS at mass resolving power 10,000 failed both to detect all of the steroid esters in hair extracts owing to the inability to mass resolve analyte ions from co-eluting isobaric matrix compounds. In a second application, animal feed extracts were fortified with coccidiostats drugs at levels ranging from 240 to 1900 ng/g. UPLC-Orbitrap MS conducted at a resolving power of 7500 and 60,000 and UPLC-TOFMS detected all of the analytes at the lowest investigated level. Thanks to the higher analyte-to-matrix background ratio, the utilization of very narrow mass tolerance windows in the RIC was not required. This study demonstrates that even when the targeted sample preparation from conventional LC-MS/MS is applied to UPLC with full-scan accurate mass MS, false compliant (false negative) results can be obtained when the mass resolving power of the MS is insufficient to separate analyte ions from isobaric co-eluting sample matrix ions. The current trend towards more generic and less selective sample preparation is expected to aggravate this issue further. (J Am Soc Mass Spectrom 2009, 20, 451-463)

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mentioning

confidence: 99%

Full-scan accurate mass selectivity of ultra-performance liquid chromatography combined with time-of-flight and orbitrap mass spectrometry in hormone and veterinary drug residue analysis

Heeft¹,

Bolck²,

Beumer³

et al. 2009

J. Am. Soc. Mass Spectrom.

128

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“…28, No. 1,2017 respectively. The samples were left to stand in the dark for 30 min at room temperature.…”

Section: Sample Preparationmentioning

confidence: 99%

“…1 Foodstuff derived from animals treated with the antibiotics fluoroquinolones (FQs) and sulfonamides may be contaminated with these drugs, which can lead to the development of allergic reactions or bacterial resistance in the consumers. 2,3 Therefore, it is vital to monitor the presence of these residues in tissues derived from food producing animals.…”

Section: Introductionmentioning

confidence: 99%

Validation of a LC-MS/MS Multiresidue Methodology Based on a QuEChERS Approach for the Determination of Fluoroquinolones, Sulfonamides and Trimethoprim in Poultry and Porcine Kidney According to the Normative Instruction 24/2009-MAPA

Rocha¹,

Santos²,

Gomes³

et al. 2016

Journal of the Brazilian Chemical Society

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This work involved the validation of a multiresidue method according to the Normative Instruction 24/2009-MAPA for determining 25 analytes, among fluoroquinolones, sulfonamides and trimethoprim in samples of poultry and porcine kidney. The extraction procedure was based on a QuEChERS approach. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed using the selected reaction monitoring mode (SRM) and ESI + ionization. All of the validated figures of merit were evaluated as satisfactory. Accuracy was assessed by recovery studies, varying from 82.7 to 115.5% for porcine kidney and from 91.5 to 110.4% for poultry kidney. Relative standard deviations were lower than 25.5% for porcine kidney, and 29.8% for poultry kidney. Decision limits (CCα) comprised values from 10.37 to 3298.43 μg kg -1 for porcine kidney and 10.08 to 3176.59 μg kg -1 for poultry kidney. Detection capabilities (CCβ) varied from 10.73 to 3396.86 μg kg -1 for porcine kidney and 10.67 to 3253.19 μg kg -1 for poultry kidney. The developed method has been successfully employed in the routine analysis of incurred samples.

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“…Antimicrobial agents are widely used in milk producing animals for therapeutic and prophylactic purposes. Apart from this, they are also used as feed additives as growth promoters may result in presence of antimicrobial residues in milk and dairy products, and can contribute to the development of microbial drug resistance and the spread of resistant bacteria (Stolker et al, 2007). Also, the residues of Veterinary Medicinal Product i.e.…”

Section: Introductionmentioning

confidence: 99%