Residual Stress Patterns Affect Cell Distributions on Injection-Molded Poly-l-Lactide Substrate

Abstract

The effects of residual intra-substrate stress distribution on cell behavior have not been systematically investigated. Thus, the objective of this research was to analyze the relationship between cell distribution and internal stress patterns. A photoelastic method was used for residual stresses identification. Poly-L-lactide (PLLA) discs were prepared using an injection molding technique. MG-63 and NIH-3T3 cells were cultured on the surface of the PLLA disc. The cell distributions for high and low-stress regions were measured and compared. There were significantly more cells in the low-stress regions relative to high-stress analogs (p < 0.05). Further, linear relationships were demonstrated for both MG-63 and NIH-3T3 models with high correlation coefficients of 0.80 and 0.95, respectively. These results suggest that the distribution of residual stress in substrates affect cell behavior. These findings may provide greater insight into the interaction between cells and substrates, and may serve as a useful reference in future clinical study.

Static magnetic field exposure promotes differentiation of osteoblastic cells grown on the surface of a poly-l-lactide substrate

“…Poly-L-lactide (PLLA) has become the most popular bioresorbable material for fixation or scaffolding because of its controlled physical and chemical surface properties [15]. In the clinical application, PLLA scaffold were applied in the dental therapy for guided tissue regeneration/guided bone regeneration as a barrier.…”

Effects of compressive residual stress on the morphologic changes of fibroblasts

“…However, for quantitative analyses, these methods make it difficult both to create controlled stress gradients in substrate samples and to accurately measure their local mechanical properties. To overcome these problems, injection-molded PLLA in combination with photoelastic measurements was proposed by Lee et al Their results suggested that this system can provide an effective system for mechanotaxis analysis [18].…”

“…While residual stress can be a mechanical stimulator for inducing bone remodeling [26], most in vitro studies of tensotaxis focused on the effects of tensile strain on cell behavior [3,23], with not much attention paid to the effects of compressive stress and strain [18]. In this study, a residual stress-controlled poly-L-lactide (PLLA) substrates, developed in our previous report [18], were used as substrates.…”

“…In this study, a residual stress-controlled poly-L-lactide (PLLA) substrates, developed in our previous report [18], were used as substrates. By seeding the fibroblasts on the substrates, the effects of the compressive stress of the substrate on cell behaviors were evaluated.…”

Development and biocompatibility tests of electrospun poly-l-lactide nanofibrous membranes incorporating oleic acid-coated Fe3O4

“…Its success greatly depends on the chemical and physical properties of the biomaterials. Recently, poly-l-lactide (PLLA) has become the most popular biodegradable material for scaffolding [1][2][3]. However, low proliferation rates and poor cell attachment were reported when cells were cultured on PLLA surfaces [4,5].…”