Residual Effects of Sucrose and Hormonal Treatments of the Tuberization Medium on <i>in Vitro</i> Germination of Potato (<i>Solanum tuberosum</i> L.) Microtubers

Abstract

The residual effects of sucrose concentrations (80 or 100 g·L ], was also lower than that one (66.66%) observed in the medium without hormones. Aida and Atlas varieties thus offer a better germination rate than Odessa after their cold storage.

Interactive effect of genotype and medium on microtuberization of potatoes (Solanum tuberosum L.) grown in vitro

“…The culture medium chosen is that of Murashige and Skoog [8], in which the macroelements are halved (MS/2). This medium is recommended by several authors [9,10,11,12] and is autoclaved at 120°C for 20 minutes at a pressure of 1 bar, its pH is adjusted to 5.7 by NaOH and HCl (1N) solutions, then solidified by agar (7g Lˉ¹ ) and supplemented with 30 g of sucrose (case of the medium used during the Micropropagation phase). The volume of the medium is 10 ml/tube and 120 ml/jar.…”

“…The BAP (6-benzyl amino purine) hormone dose (2.5 mg/l) is recommended by LÊ and Thomas [7], whereas the dose of kinetin (2.5 mg/l) and coumarin ( 25Corresponding author: kadizahia6@yahoo.fr Full Paper DOI: 10.2478/asn-2020-0003 ©2020 Konstantin Preslavsky University of Shumen. All rights reserved mg/l) is recommended by Diémé et al [12]. To facilitate their use, BAP and kinetin are dissolved in a few drops of NaOH, while coumarin is dissolved in 96% ethanol.…”

Environmental, morphological and physiological factors analyzes for optimization of potato (<i>Solanum tuberosum</i> L.) microtuber <i>in vitro</i> germination

“…The microtubers were aseptically seeded in culture tubes filled with an 8% agarified MS (0) medium at pH 5.9 [9] and then incubated in the dark at different temperatures (Plate 1). The culture media were prepared and conditioned as previously described [10]. A Plate 1.…”

Yield and seed productivity of pre-basic seed material depending potatoes on growing technology elements

“…äîâåäåíî, ùî çàñòîñóâàííÿ ð³ñòðåãóëþþ÷èõ ðå-÷îâèí ñïðèÿëî ï³äâèùåííþ ñò³éêîñò³ êàðòîï-ë³ äî ðàííüîãî òà ï³çíüîãî ïðîÿâó ô³òîôòîðîçó, çðîñòàííþ óðîaeàéíîñò³ áóëüá òà ïîêðàùåííþ ôðàêö³éíîãî ñêëàäó âðîaeàþ [4]. ×èñëåííèìè äîñë³äaeåííÿìè âñòàíîâëåíî ïîçèòèâíèé âïëèâ çàñòîñóâàííÿ ð³ñòðåãóëþ-þ÷èõ ðå÷îâèí íà ïîêàçíèêè ðîñòó òà ðîçâèòêó ðîñëèí êàðòîïë³ in vitro çà ì³êðîêëîíàëüíîãî ðîçìíîaeåííÿ [5][6][7][8][9][10]. Çà âèçíà÷åííÿ âïëèâó ð³ñòðåãóëÿòîð³â íà ïðîäóêòèâí³ñòü îçäîðîâëåíî¿ êàðòîïë³ íà ðîçñàäí³é êóëüòóð³ âñòàíîâëåíî, ùî ïîçàêîðåíåâå ï³äaeèâëåííÿ ã³áåðåë³íîì À 3 â äîç³ 20 ã/ãà ï³äâèùóâàëî âðîaeàéí³ñòü êàðòîïë³ íà 19-26%, çá³ëüøóâàëî êîåô³ö³ºíò ðîçìíîaeåííÿ ðîñëèí íà 18-22%, âèõ³ä áóëüá ç îäèíèö³ ïëîù³ íà 17-18%.…”