Residual DNA from Hybridoma Cultures: Decorrence of Apoptosis?

Animal cell technology: from biopharmaceuticals to gene therapy

“…These facts, taken together with other evidence (Kurt, 1995;Petricciani and Horaud, 1995;Temin, 1990), led to the argument that the risk associated with residual cellular DNA is low when the amount is 100 pg per dose or less. An important aspect is that residual contaminant DNA usually occurs in small fragments of 200-500 bp (Rodrigues et al, 1997), thus not codifying a functional gene.…”