Residual Content of Persistent Insecticides in the Fresh Nuts of Cola Acuminata Cohac from Kumbotso Local Government Area of Kano State and Implication for Public Health

Abstract

The pesticide residues in kola nut, Cola acuminata obtained from Mariri Market, Kumbotso Local Government Area, Kano state of Nigeria was investigated. The study consisted of a survey and laboratory analysis of kola nut samples. One hundred structured questionnaires were administered through an open market format in the major kola nut market in the state. In addition, 1kg sample of kola nut was collected randomly from ten major kola nut sellers in the market for analysis. A 100g sample of the kola nut was pounded into paste in a blender of which 50g was soaked in 150mL acetone for 24h. The extracted sample was filtered through anhydrous sodium sulfate while the extract was evaporated to near dryness using a rotary evaporator at 28oC in the Laboratory. Residue was redissolved in 2 mL acetone and kept in the refrigerator for GCMS Analysis. Ten pesticidal compounds were detected from a comparison of data with reference to mass spectrometry data (NIST05.LIB) and significant occurrence in kola nut sample which had a corresponding molecules affiliated to some pesticides standards were detected in the sample