Resident vascular progenitor cells: An emerging role for non-terminally differentiated vessel-resident cells in vascular biology

Kovacic, Jason C.; Boehm, Manfred

doi:10.1016/j.scr.2008.05.005

Cited by 70 publications

(48 citation statements)

References 119 publications

(165 reference statements)

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“…It is notable that unlike most methods that selectively isolate ENSCs from the gut layer containing myenteric plexus, Suarez-Rodriguez et al (33) used the entire gut for their preparations therefore it also contained the SP layer. While CD34 has traditionally been used as a marker of hematopoietic stem cells (29), there is emerging evidence that it also marks other mesenchymal stem cell populations including ICC progenitors, pericyte progenitors, vascular progenitors, and adipose tissue-derived stem cells (12,15,19,22,34). Certainly further characterization of these nonneural crest derived cells is warranted.…”

Section: Discussionmentioning

confidence: 99%

Divergent fate and origin of neurosphere-like bodies from different layers of the gut

Becker

Kulkarni

Tiwari

et al. 2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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Enteric neural stem cells (ENSCs) are a population of neural crest-derived multipotent stem cells present in postnatal gut that may play an important role in regeneration of the enteric nervous system. In most studies, these cells have been isolated from the layer of the gut containing the myenteric plexus. However, a recent report demonstrated that neurosphere-like bodies (NLBs) containing ENSCs could be isolated from mucosal biopsy specimens from children, suggesting that ENSCs are present in multiple layers of the gut. The aim of our study was to assess whether NLBs isolated from layers of gut containing either myenteric or submucosal plexus are equivalent. We divided the mouse small intestine into two layers, one containing myenteric plexus and the other submucosal plexus, and assessed for NLB formation. Differences in NLB density, proliferation, apoptosis, neural crest origin, and phenotype were investigated. NLBs isolated from the myenteric plexus layer were present at a higher density and demonstrated greater proliferation, lower apoptosis, and higher expression of nestin, p75, Sox10, and Ret than those from submucosal plexus. Additionally, they contained a higher percentage of neural crest-derived cells (99.4 ± 1.5 vs. 0.7 ± 1.19% of Wnt1-cre:tdTomato cells; P < 0.0001) and produced more neurons and glial cells than those from submucosal plexus. NLBs from the submucosal plexus layer expressed higher CD34 and produced more smooth muscle-like cells. NLBs from the myenteric plexus layer contain more neural crest-derived ENSCs while those from submucosal plexus appear more heterogeneous, likely containing a population of mesenchymal stem cells.

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Section: Discussionmentioning

confidence: 99%

Divergent fate and origin of neurosphere-like bodies from different layers of the gut

Becker

Kulkarni

Tiwari

et al. 2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…[35][36][37][38] Although the precise definition, characteristics, and origin of endothelial and vascular progenitor cells remain controversial, their overall importance appears clear. 36 An emerging theme of this work is the mounting evidence that with age, stem cell numbers and various aspects of stem cell function are reduced, or become exhausted.…”

Section: Age-related Decline In Stem Cell Number and Functionmentioning

confidence: 99%

“…[35][36][37][38] Même si les opinions divergent concernant la définition, les caractéristiques et l'origine précises des progéniteurs endothéliaux et vasculaires, leur importance globale est un fait désormais acquis. 36 Une notion commence à émerger de la somme croissante de données émanant de la recherche, qui est que, avec l'avancée en l'âge, le nombre des cellules souches et certaines de leurs fonctionnalités diminuent ou s'épuisent.…”

Section: Cellules Souches Vasculaires Et Progérine Diminution Du Nombunclassified

Cellular Senescence, Vascular Disease, and Aging

Kovacic

Moreno²,

Hachinski³

et al. 2011

Circulation

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159

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Longevity is a vascular question, which has been well expressed in the axiom that man is only as old as his arteries. To a majority of men death comes primarilyn 2030, when all of the baby boomer generation will be Ն65 years of age, nearly 1 in 5 US residents is expected to be Ͼ65 years of age. By 2050, this age group is projected to more than double in number, from 38.7 million in 2008 to an estimated 88.5 million (Figure 1). 2 Similarly, the population Ն85 years of age is expected to more than triple, from 5.4 million in 2008 to 19 million by 2050. 2 With this aging of the population, the number of people at risk for adverse cardiovascular events, in particular atherothrombosis, stroke, myocardial infarction, and heart failure, will increase dramatically. The importance of these projections is underscored by the fact that currently, although octogenarians represent only 5% of the US population, they account for 20% of all hospitalizations for myocardial infarction and 30% of all myocardial infarction-related deaths. 3 A Looming Aging EpidemicDespite their increased risk of adverse cardiac events, elderly patients are less likely to receive appropriate therapy. This paradox is perhaps related to the fact that elderly patients with cardiovascular disease are more likely to be frail. 4 Heightened concerns that frail older patients may be more susceptible to adverse side effects, particularly in the setting of complex pharmacotherapy, can lead to efficacious therapies being withheld. [5][6][7] As an example, although the relative risk reduction of cholesterol-lowering therapy may be equivalent in the young and elderly, the absolute risk reduction, or number of adverse cardiovascular events prevented, may be significantly greater in elderly patients. 8 This serves as a sobering reminder that even though many exciting advances that might help fight the ravages of old age appear to be at the point of clinical translation, at the present time, clinicians must remain vigilant to ensure the delivery of best medical care to all elderly patients.In this 2-part review, we discuss important pathobiological changes that occur with aging in the cardiovascular system and how they relate to clinical outcomes. Furthermore, we explore areas of scientific progress that may, optimistically, be translatable into targeted clinical strategies to help offset the looming impact of the aging epidemic on morbidity, mortality, and healthcare resource use. Readers should be aware that we do not propose to exhaustively discuss all potential biological players in this complex arena. Rather, we will attempt to selectively highlight novel and important broad aspects of basic and cellular biology and clinical medicine that are of significant relevance to this field. In this first installment, we focus at the basic-science level on key advances in our understanding of the cellular aging process and look to extend these observations to the aging adult vasculature. Telomere Shortening and Cellular Senescence Mechanisms of Telomere-Telomerase Fun...

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“…15,18,36 In addition to their multilineage potential, they exert a potent angiogenic effect through either the adoption of an endothelial-or a PC-like phenotype. Campagnolo et al 37 isolated a population of CD34 þ CD31 À progenitor cells from the adventitia of human saphenous vein-derived progenitor cells.…”

Section: Mpcs Are Capillary-forming Stem Cellsmentioning

confidence: 99%

Immortalized multipotent pericytes derived from the vasa vasorum in the injured vasculature. A cellular tool for studies of vascular remodeling and regeneration

Kabara

Kawabe

Matsuki

et al. 2014

Laboratory Investigation

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Adventitial microvessels, vasa vasorum in the vessel walls, have an active role in the vascular remodeling, although its mechanisms are still unclear. It has been reported that microvascular pericytes (PCs) possess mesenchymal plasticity. Therefore, microvessels would serve as a systemic reservoir of stem cells and contribute to the tissues remodeling. However, most aspects of the biology of multipotent PCs (mPCs), in particular of pathological microvessels are still obscure because of the lack of appropriate methods to detect and isolate these cells. In order to examine the characteristics of mPCs, we established immortalized cells residing in adventitial capillary growing at the injured vascular walls. We recently developed in vivo angiogenesis to observe adventitial microvessels using collagen-coated tube (CCT), which also can be used as an adventitial microvessel-rich tissue. By using the CCT, CD146-or NG2-positive cells were isolated from the adventitial microvessels in the injured arteries of mice harboring a temperature-sensitive SV40 T-antigen gene. Several capillary-derived endothelial cells (cECs) and PCs (cPCs) cell lines were established. cECs and cPCs maintain a number of key endothelial and PC features. Co-incubation of cPCs with cECs formed capillary-like structure in Matrigel. Three out of six cPC lines, termed capillary mPCs demonstrated both mesenchymal stem cell-and neuronal stem cell-like phenotypes, differentiating effectively into adipocytes, osteoblasts, as well as schwann cells. mPCs differentiated to ECs and PCs, and formed capillary-like structure on their own. Transplanted DsRed-expressing mPCs were resident in the capillary and muscle fibers and promoted angiogenesis and myogenesis in damaged skeletal muscle. Adventitial mPCs possess transdifferentiation potential with unique phenotypes, including the reconstitution of capillary-like structures. Their phenotype would contribute to the pathological angiogenesis associated with vascular remodeling. These cell lines also provide a reproducible cellular tool for high-throughput studies on angiogenesis, vascular remodeling, and regeneration as well.

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Resident vascular progenitor cells: An emerging role for non-terminally differentiated vessel-resident cells in vascular biology

Cited by 70 publications

References 119 publications

Divergent fate and origin of neurosphere-like bodies from different layers of the gut

Divergent fate and origin of neurosphere-like bodies from different layers of the gut

Cellular Senescence, Vascular Disease, and Aging

Immortalized multipotent pericytes derived from the vasa vasorum in the injured vasculature. A cellular tool for studies of vascular remodeling and regeneration

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