Resident aliens: the Tc1/ mariner superfamily of transposable elements

Plasterk, Ronald H.A.; Izsvák, Zsuzsanna; Ivics, Zoltán

doi:10.1016/s0168-9525(99)01777-1

Cited by 434 publications

(398 citation statements)

References 80 publications

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“…Most consist of transposase gene flanked by terminal inverted repeats. Some demonstrate a requirement for species specific host factors while others are active between species and in some cases between kingdoms (reviewed in Plasterk et al, 1999). Endogenous cut and paste transposons have been found in vertebrates though most if not all appear to have degenerated due to loss of function mutations.…”

Section: Dna Transposonsmentioning

confidence: 99%

Retroviral insertional mutagenesis: past, present and future

et al. 2005

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Retroviral insertion mutagenesis screens in mice are powerful tools for efficient identification of oncogenic mutations in an in vivo setting. Many oncogenes identified in these screens have also been shown to play a causal role in the development of human cancers. Sequencing and annotation of the mouse genome, along with recent improvements in insertion site cloning has greatly facilitated identification of oncogenic events in retrovirus-induced tumours. In this review, we discuss the features of retroviral insertion mutagenesis screens, covering the mechanisms by which retroviral insertions mutate cellular genes, the practical aspects of insertion site cloning, the identification and analysis of common insertion sites, and finally we address the potential for use of somatic insertional mutagens in the study of nonhaematopoietic and nonmammary tumour types.

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Section: Dna Transposonsmentioning

confidence: 99%

Retroviral insertional mutagenesis: past, present and future

et al. 2005

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“…This feature is recognized also in eukaryotic Tc1/mariner family elements (Plasterk et al, 1999). Moreover, Tc1/mariner family elements, like IS630 family elements, also have conserved DDE or DDD triad amino acids as an essential part of their catalytic site, and mutations in the triad abolished transposase activity (Lohe et al, 1997).…”

Section: Discussionmentioning

confidence: 97%

A new insertion sequence, IS14999, from Corynebacterium glutamicum

et al. 2005

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A new insertion sequence from Corynebacterium glutamicum ATCC 14999 was isolated and characterized. This IS element, designated IS14999, comprised a 1149 bp nucleotide sequence with 22 bp imperfect terminal inverted repeats. IS14999 carries a single open reading frame of 345 amino acids encoding a putative transposase that appears to have partial homology to IS642, an IS630/Tc1 superfamily element, at the C-terminal region in the amino acid sequence. This indicated that IS14999 belonged to the IS630/Tc1 superfamily, which was first identified in C. glutamicum. IS14999 has a unique distance of 38 amino acid residues between the second and third amino acids in the DDE motif, which is well known as the catalytic centre of transposase. This suggested that IS14999 constituted a new subfamily of the IS630/Tc1 superfamily. A phylogenetic tree constructed on the basis of amino acid sequences of transposases revealed that this new transposable element was more similar to eukaryotic Tc1/mariner family elements than to prokaryotic IS630 family elements. Added to the fact that IS14999 was present in only a few C. glutamicum strains, this implies that IS14999 was probably acquired by a recent lateral transfer event from eukaryotic cells. Analysis of the insertion site in C. glutamicum R revealed that IS14999 appeared to transpose at random and always caused a target duplication of a 59-TA-39 dinucleotide upon insertion, like the other IS630/Tc1 family elements. These findings indicated that IS14999 could be a powerful tool for genetic manipulation of corynebacteria and related species.

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“…The lower frequency of Minos excision compared with SB but its comparable frequency of germline transformation suggests, in other words, that a Minos copy excised from plasmids has a higher chance than SB to be integrated into another DNA in C. intestinalis. Minos and SB are classified in the same Tc1/mariner superfamily of transposons (Plasterk et al, 1999), and these transposons have multiple shared characters. However, their transposition mechanisms are expected to be different, which may cause the differences in excision and transposition activity between SB and Minos.…”

Section: Developmental Dynamicsmentioning

confidence: 99%

Germline transgenesis of the chordate Ciona intestinalis with hyperactive variants of sleeping beauty transposable element

Hozumi

Mita

Miskey

et al. 2012

Developmental Dynamics

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Background: Transposon-mediated transgenesis is an excellent method for creating stable transgenic lines and insertional mutants. In the chordate Ciona intestinalis, Minos is the only transposon that has been used as the tool for germline transformation. Adding another transposon system in this organism enables us to conduct genetic techniques which can only be realized with the use of two transposons. Results: In the present study, we found that another Tc1/mariner superfamily transposon, sleeping beauty (SB), retains sufficient activity for germline transformation of C. intestinalis. SB shows efficiencies of germline transformation, insertion into gene coding regions, and enhancer detection comparable to those of Minos. We have developed a system for the remobilization of SB copies in the C. intestinalis genome by using transgenic lines expressing SB transposase in the germ cells. With this system, we examined the manner of SB mobilization in the C. intestinalis genome. SB shows intrachromosomal transposition more frequently than Minos. Conclusions: SB-based germline transformation and the establishment of a new method that uses its frequent intrachromosomal transposition will result in breakthroughs in genetic approaches that use C. intestinalis together with Minos. Developmental Dynamics 242:30-43, 2013. V C 2012 Wiley Periodicals, Inc.Key words: ascidian; Ciona intestinalis; transposon; Tc1; mariner; sleeping beauty; Minos Key findings:In C. intestinalis, Minos is the only transposon that has been used as a tool for germline transformation. We found that another transposon, sleeping beauty (SB), retains sufficient activity for the germline transformation of C. intestinalis. SB-based germline transformation will result in breakthroughs in genetic approaches that use C. intestinalis together with Minos.

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Resident aliens: the Tc1/ mariner superfamily of transposable elements

Cited by 434 publications

References 80 publications

Retroviral insertional mutagenesis: past, present and future

Retroviral insertional mutagenesis: past, present and future

A new insertion sequence, IS14999, from Corynebacterium glutamicum

Germline transgenesis of the chordate Ciona intestinalis with hyperactive variants of sleeping beauty transposable element

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