Resected Early Gastric Cancer. Clinicopathological Studies on 610 Cases.

Takeda, Jinryo; Toyonaga, Atsushi; Koufuji, Kikuo; Kodama, Issei; Aoyagi, Keishiro; Ohta, Junji; Aoyama, Yuko; Hata, Hirofumi

doi:10.2739/kurumemedj.42.87

Cited by 7 publications

(2 citation statements)

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“…Glucocorticoid and leukotriene receptor antagonists (LTRA) are the most commonly used drugs in the therapy of asthma [ 33 ].The abilities of dexamethasone and fluticasone propionate to inhibit MMP-2 expression, which has been associated with airway remodeling, induced by cigarette smoke extract have been demonstrated [ 34 ]. Budesonide can inhibit chlorine-induced airway fibrosis [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

IL-1β augments TGF-β inducing epithelial-mesenchymal transition of epithelial cells and associates with poor pulmonary function improvement in neutrophilic asthmatics

Zhang

Fan²,

Qin

et al. 2021

Respir Res

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Background Neutrophilic asthmatics (NA) have less response to inhaled corticosteroids. We aimed to find out the predictor of treatment response in NA. Methods Asthmatics (n = 115) and healthy controls (n = 28) underwent clinical assessment during 6-month follow-up with standardized therapy. Asthmatics were categorized by sputum differential cell count. The mRNA expressions were measured by RT-qPCR for sputum cytokines (IFN-γ, IL-1β, IL-27, FOXP3, IL-17A, and IL-5). The protein of IL-1β in sputum supernatant was detected by ELISA. Reticular basement membranes (RBM) were measured in the biopsy samples. The role and signaling pathways of IL-1β mediating the epithelial-mesenchymal transition (EMT) process were explored through A549 cells. Results NA had increased baseline sputum cell IL-1β expression compared to eosinophilic asthmatics (EA). After follow-up, NA had less improvement in FEV1 compared to EA. For all asthmatics, sputum IL-1β mRNA was positively correlated with protein expression. Sputum IL-1β mRNA and protein levels were negatively correlated to FEV1 improvement. After subgrouping, the correlation between IL-1β mRNA and FEV1 improvement was significant in NA but not in EA. Thickness of RBM in asthmatics was greater than that of healthy controls and positively correlated with neutrophil percentage in bronchoalveolar lavage fluid. In vitro experiments, the process of IL-1β augmenting TGF-β1-induced EMT cannot be abrogated by glucocorticoid or montelukast sodium, but can be reversed by MAPK inhibitors. Conclusions IL-1β level in baseline sputum predicts the poor lung function improvement in NA. The potential mechanism may be related to IL-1β augmenting TGF-β1-induced steroid-resistant EMT through MAPK signaling pathways. Trial registration: This study was approved by the Ethics Committee of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (IRB ID: 20150406).

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