Researchers of chronic obstructive pulmonary disease gathered at the 2017 Japan-China Joint Medical Workshop on Aging and Health

Sawakami, Tatsuo; Xia, Jufeng; Song, Peipei

doi:10.5582/bst.2017.01323

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…In China, the combination of antiviral treatment, such as Tamiflu and traditional Chinese medicines (TCMs), accounted for a large portion and was regarded to have unique advantage in treating EIDs to improve the clinical therapeutic effectiveness in virus prevention and control . The ShuFengJieDu capsule (SFJD), consisting of Rhizoma polygoni cuspidati, Fructus forsythiae, Radix isatidis, Radix bupleuri, Herba patriniae, Herba verbenae, Rhizoma phragmitis, and Radix glycyrrhizae, is one type of TCM for treating upper respiratory infection (URI) with symptoms of fever, sore throat, headache, and cough. − A clinical study had demonstrated that administration of SFJD abates fever in an average time of 20.5 h, implying that SFJD had rapid and effective antipyretic action . In our previous study, we found that the presence of SFJD increased the partial pressure of oxygen in lung tissue, decreased lactic acid levels, and inhibited inflammatory factors such as IL-1β and TNF-α in acute lung injury rats.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Proteomics Analysis of Systemic Responses and Biological Mechanisms of ShuFengJieDu Capsule Using H1N1-Infected RAW264.7 Cells

Tao

et al. 2020

ACS Omega

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Emerging infectious diseases (EIDs) are a significant burden on global economies and public health to any country in the world. With the extensive application of traditional Chinese medicines (TCMs) for EID treatment, the underlying molecular mechanisms have caught more attention than before. The ShuFengJieDu capsule (abbreviated as SFJD) is a TCM prescription used for treating upper respiratory infection (URI) with symptoms of fever, sore throat, headaches, nasal congestion, and cough for more than 30 years in China. SFJD is also widely used for the prevention and treatment of viral infectious diseases, especially for the EIDs. In this study, a bioactivity-integrated method of ultraperformance liquid chromatography quadrapole/time-of-flight mass spectrometry combined with methyl thiazolyl tetrazolium assay was applied to screen potential antivirus compounds in SFJD on the H1N1-infected RAW264.7 cell models. Three compounds (forsythoside E, verbenalin, and emodin) exert the advantages of protective effects in cell vitality during H1N1 infection. The isobaric tags for relative and absolute quantification (iTRAQ)-coupled liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) analysis and the subsequent quantitative proteome analysis were performed to investigate the potential molecular mechanisms triggered by these three bioactive compound-triggered molecular mechanisms in H1N1-infected RAW264.7 cells. Dysregulated proteins were involved in regulating the levels of proinflammatory cytokines, the IFN (interferon)-stimulated gene signal in the Type I IFN, TBK/IRF3, and MAPK/NF-κB signaling pathways. In conclusion, we identified the main bioactive compounds in SFJD exerting antiviral effects and illuminated that Type I IFN and MAPK/NF-κB signaling pathways are involved in the anti-H1N1 infection effects of SFJD. Our study not only provides solid theoretical support for the clinic application of SFJD but also sheds light on the novel research methods for TCM study.

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