Research progress on the correlation between gestational vaginal microecology and vaginal infectious diseases

Dong, Haiwei; Yang, Xiaohui

doi:10.2478/ii-2018-0007

Cited by 3 publications

(4 citation statements)

References 5 publications

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“…The imbalance of vaginal microecology is governed by the etiological, environmental and host factors. Pregnancy is a special physiological period, during which the physiological hormonal variations easily cause changes in the vaginal flora environment, thereby resulting in imbalance of vaginal microecology [ 9 , 10 ]. This greatly increases the risk of various types of vaginal inflammation.…”

Section: Discussionmentioning

confidence: 99%

Effects of vaginal microecology and immunity on the pregnancy outcome of cervical cerclage

Cai

Zeng

et al. 2022

BMC Women's Health

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Objective To investigate the effects of vaginal microecology and immune status on the pregnancy outcome of cervical cerclage. Methods The clinical data of 125 patients with cervical incompetence who underwent transvaginal cervical cerclage in our hospital from January 2018 to January 2021 were collected, based on which the associations of vaginal microecology and related immune cytokines (IL-1β, IL-6, IL-8 and TNF-α) with the cervical cerclage outcome were explored. Results All of the 125 patients had singleton pregnancies, who were aged 20–43 years, with a mean of (32.34 ± 5.17) years. The surgery was successful in 104 patients (full-term delivery or survival of premature infants), while unsuccessful in 21 patients (late miscarriage or death of premature infants), revealing a success rate of 83.20%. There were 70 full-term deliveries, 34 premature deliveries (28 survived while 6 died), and 15 late miscarriages. Univariate analysis revealed statistically significant differences in the timing of surgery, gestational age of cerclage, preoperative cervical canal length, genital tract infections and preoperative invasive procedures between the successful and unsuccessful groups (P < 0.05). Insignificant differences were found in the pre-pregnancy body mass index (BMI) (P > 0.05). According to the multivariate analysis results, cerclage timing and genital tract infections were independent risk factors for postoperative pregnancy failure (P < 0.05). The pathogen detection rates in the two groups of pregnant women were analyzed, finding significantly higher incidence of bacterial vaginosis (BV) in the unsuccessful group than in the successful group (P < 0.05). Inter-group comparison revealed that the positive rates for vaginal microenvironmental factors (LE, NAG, SNA, H2O2 and pH) were all significantly higher in the unsuccessful group than in the successful group (P < 0.05). Besides, the immune cytokine levels in the cervicovaginal secretions were also all significantly higher in the unsuccessful group than in the successful group (P < 0.05). Conclusion The pregnancy outcome of patients undergoing cervical cerclage is associated with the imbalance of vaginal microecology and the levels of IL-1β, IL-6, IL-8 and TNF-α in cervicovaginal secretions.

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Section: Discussionmentioning

confidence: 99%

Effects of vaginal microecology and immunity on the pregnancy outcome of cervical cerclage

Cai

Zeng

et al. 2022

BMC Women's Health

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“…At present, Lactobacillus vaginalis live capsules (Dingjunsheng) are mainly used for the treatment of bacterial vaginosis (BV) and have achieved certain effect, but the Lactobacillus delbrueckii is not the dominant vaginal microbiota in Chinese women, which has poor colonization ability and cannot maintain stable live bacteria content [24]. L. crispatus used in this study is from healthy women and has strong ability of acid production, hydrogen peroxide production and adhesion with vaginal epithelial cells, which is effective in resisting bacterial vaginosis and various vaginal infections, as well as having the characteristics of safe, non-toxic, stable and long-term preservation [25].…”

Section: Introductionmentioning

confidence: 99%

Improvement of vaginal probiotics Lactobacillus crispatus on intrauterine adhesion in mice model and in clinical practice

Kong

Chen

et al. 2023

Preprint

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Background Intrauterine adhesion (IUA) is a frequent acquired endometrial condition, for which there is no effective preventive or treatment. Previous studies have found that vaginal microbiota dysregulation is closely related to endometrial fibrosis and IUA. Results In this study, we administered Lactobacillus crispatus (L. crispatus) vaginally to restore vaginal microbiota and explore the beneficial role of vaginal probiotics in the treatment and prevention of IUA. Then, we created a mechanically injured mouse model of IUA and restored the mice's vaginal microbiota by the addition of L. crispatus convolvulus. The observations suggested that intrauterine injections of L. crispatus significantly decreased the degree of uterine fibrosis, the levels of IL-1β and TNF-α in blood, and downregulated the TGF-β1/SMADs signaling pathway in IUA mice. A therapy with L. crispatus considerably raised the abundance of the helpful bacteria Lactobacillus and Oscillospira and restored the balance of the vaginal microbiota in IUA mice, according to high-throughput sequencing. Then we conducted a randomized controlled trial to compare the therapeutic effect of L. crispatus with estrogen after TCRA. And the results showed that vaginal probiotics had a better potential to prevent intrauterine adhesion than estrogen. Conclusions This study confirmed that the prevention and treatment of L. crispatus in IUA has a certain effect, and made a new exploration for the treatment of vaginal probiotics in gynecological diseases. Clinical trial registration : http://www.chictr.org.cn/, identifier (ChiCTR1900022522), registration time: 15/04/2019.

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“…There are more than 50 types of microorganisms that colonize the vaginal mucosa of healthy women. They interact with each other to form a biofilm 6 . Amplicon sequencing of the 16S ribosomal RNA (rRNA) gene has proven to be a powerful strategy for the taxonomic classification of bacteria 7 …”

Section: Introductionmentioning

confidence: 99%

“…They interact with each other to form a biofilm. 6 Amplicon sequencing of the 16S ribosomal RNA (rRNA) gene has proven to be a powerful strategy for the taxonomic classification of bacteria. 7 At present, studies concerned with the effects of reproductive tract microbiota on reproduction and assisted reproduction mainly focus on in vitro fertilization and embryo transfer (IVF-ET).…”

mentioning

confidence: 99%

Associations between vaginal flora, MIP‐1α, IL‐17A, and clinical pregnancy rate in AIH

Yang

Gao

Wang

et al. 2022

American J Rep Immunol

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Problem To investigate how asymptomatic bacterial imbalance affects the clinical pregnancy rate after artificial insemination with the husband's semen (AIH). Methods This study included married heterosexual couples who underwent AIH. According to the follow‐up results, participants were divided into the pregnancy and non‐pregnancy groups. Based on the first 10 pair participants in each group with vaginal flora bacterial 16S rRNA sequencing results, six semen samples received bacterial‐sperm mixed test. Moreover, 34 cytokines were detected in the peripheral blood sera of the first three pairs by high‐throughput Luminex, which were verified in vaginal secretions, cervical mucus, and blood sera from the first 200 pairs by ELISA. Results The results of the 16S sequencing of vaginal secretions showed that compared with the pregnant group, the non‐pregnant group had a significantly increased bacterial species diversity, which was mainly manifested by a decrease in Lactobacillus crispatus and an increase in Prevotella bivia. When Prevotella bivia or Lactobacillus crispatus were mixed with sperms, the sperm motility was decreased (p < .05). The vaginal posterior fornix secretions, cervical mucus, and peripheral blood sera of the non‐pregnant group showed decreased levels of MIP‐1α and increased levels of IL‐17A (p < .05). Conclusion The imbalance of vaginal flora leading to the increase of Prevotella bivia and the decrease of Lactobacillus crispatus may cause an imbalance of immune regulation. Low expression of MIP‐1α and high expression of IL‐17A were associated with reduced clinical pregnancy rate in AIH.

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Research progress on the correlation between gestational vaginal microecology and vaginal infectious diseases

Cited by 3 publications

References 5 publications

Effects of vaginal microecology and immunity on the pregnancy outcome of cervical cerclage

Effects of vaginal microecology and immunity on the pregnancy outcome of cervical cerclage

Improvement of vaginal probiotics Lactobacillus crispatus on intrauterine adhesion in mice model and in clinical practice

Associations between vaginal flora, MIP‐1α, IL‐17A, and clinical pregnancy rate in AIH

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