Research progress on the active ingredients of traditional Chinese medicine in the intervention of atherosclerosis: A promising natural immunotherapeutic adjuvant

Jing, Jinpeng; Zhu, Chaojun; Gong, Rui; Xue, Qi; Zhang, Yue; Zhang, Zhaohui

doi:10.1016/j.biopha.2022.114201

Cited by 11 publications

(8 citation statements)

References 216 publications

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“…TCM is composed of multiple ingredients with diverse pharmacological activities and various targets and pathways [ 23 ]. Accumulating evidence suggests that TCM may have excellent effects on AS treatment [ 24 – 26 ]. Nevertheless, elucidating the underlying mechanisms of TCM in the treatment of AS remains challenging.…”

Section: Discussionmentioning

confidence: 99%

Network pharmacology and in vivo evidence of the pharmacological mechanism of geniposide in the treatment of atherosclerosis

Ma,

Dong,

et al. 2024

BMC Complement Med Ther

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Background Atherosclerosis (AS) is a fundamental pathological state in various cardiovascular diseases. Geniposide, which is the main active component of Gardenia jasminides, is effective against AS. However, the underlying molecular mechanisms remain unclear. Here, we sought to elucidate them. Methods The targets of AS and geniposide were collected from online public databases. The potential mechanism of Geniposide in treating AS was predicted by constructing a protein–protein interaction (PPI) network and conducting Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment analyses. Hub proteins and core pathways were verified by molecular docking and in vivo experiments. Moreover, the effect of geniposide on AS was assessed by measuring the atherosclerotic plaque area in the thoracic aorta of mice. ApoE−/− mice were used to establish AS models and randomly divided into different groups. Two different doses of geniposide were administered to the mice. Hematoxylin and eosin (HE) staining was performed to evaluate the effects of geniposide on AS. Oil Red O and Sirius Red staining were used to evaluate plaque stability. The protein expression of key markers involved in the signalling pathways was examined using western blotting and immunofluorescence. Results A total of 239 active targets, 3418 AS-related disease targets, and 129 overlapping targets were identified. Hub genes were detected, and molecular docking revealed that geniposide strongly interacted with hub proteins (AKT1, VEGFA, CTNNB1, MMP9, and EGFR). Moreover, 109 signalling pathways, including the Rap1 signalling pathway, were identified using enrichment analysis. The results of in vivo experiments demonstrated that geniposide reduced body weight and blood lipid levels, alleviated the formation of atherosclerotic plaques, enhanced plaque stability, and inhibited inflammation, at least partially, by activating the Rap1/PI3K/Akt signalling pathway in ApoE−/− mice. Conclusion Geniposide can alleviate AS and enhance the stability of atherosclerotic plaques by regulating the Rap1/PI3K/Akt signalling pathway.

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Section: Discussionmentioning

confidence: 99%

Network pharmacology and in vivo evidence of the pharmacological mechanism of geniposide in the treatment of atherosclerosis

Ma,

Dong,

et al. 2024

BMC Complement Med Ther

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“… 66 Moreover, active ingredients derived from traditional Chinese medicine showcase the potential to modulate immune cell function in diverse ways to combat AS, highlighting promise as vaccine adjuvants. 67 Given the challenges in developing new vaccine adjuvants, enhancing the formulation and optimizing the performance of classic vaccine adjuvants like alum presents a feasible approach. This can be achieved through surface modification, combined utilization with other adjuvants, and integration with new technologies such as nanoliposomes and lasers to enhance the immune efficacy while upholding the safety profile of classic adjuvants.…”

Section: Discussionmentioning

confidence: 99%

A bibliometric analysis of vaccination against atherosclerosis

Jia,

Wei,

Yuan

et al. 2024

Human Vaccines & Immunotherapeutics

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“…Additionally, natural compounds often have a richer structural and biological profile, making them an increasingly attractive resource for drug development [6]. Numerous studies have shown that the integration of TCM into the clinical treatment of tumors can significantly enhance treatment outcomes and reduce the occurrence of chemotherapy-related side effects [7,8]. Jianpi Jiedu Recipe (JPJDR) is a traditional Chinese medicine formula that is commonly used in China to treat gastrointestinal tumors.…”

Section: Introductionmentioning

confidence: 99%

Jianpi Jiedu Recipe inhibits proliferation through reactive oxygen species-induced incomplete autophagy and reduces PD-L1 expression in colon cancer

Cheng,

Xu,

Yuan

et al. 2024

Preprint

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Background: Jianpi Jiedu Recipe has been used to treat digestive tract tumors in China since ancient times, and its reliability has been proven by clinical research. Currently, the specific biological mechanism of JPJDR in treating tumors is unclear. Methodology: CCK-8 assay was used to detect cell viability. Clone formation assay and EdU assay were used to detect cell proliferation potential. DCFH-DA probe and JC-1 probe were used to detect total intracellular reactive oxygen species and mitochondrial membrane potential, respectively. Western blotting and immunofluorescence were used to detect protein expression level and subcellular localization of cells. RFP-GFP-LC3B reporter system was used to observe the type of autophagy in cells. Xenograft tumor model was used to study the therapeutic effect of JPJDR in vivo. Results: JPJDR has a good inhibitory effect on various colorectal cancer cells and effectively reduces the proliferation ability of HT29 cells. After treatment with JPJDR, the amount of reactive oxygen species in HT29 cells increased significantly and the mitochondrial membrane potential decreased. JPJDR induced the accumulation of autophagosomes in HT29 cells and was shown to be incomplete autophagy. At the same time, JPJDR reduced the expression of PD-L1.Meanwhile, JPJDR can exert a good therapeutic effect in xenograft tumor mice. Conclusion: JPJDR is a low-toxic and effective anti-tumor agent that can effectively treat colon cancer in vitro and in vivo. Its mechanism may be through inducing mitochondrial dysfunction and incomplete autophagy injury to inhibit the proliferation of colon cancer cells.

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Research progress on the active ingredients of traditional Chinese medicine in the intervention of atherosclerosis: A promising natural immunotherapeutic adjuvant

Cited by 11 publications

References 216 publications

Network pharmacology and in vivo evidence of the pharmacological mechanism of geniposide in the treatment of atherosclerosis

Network pharmacology and in vivo evidence of the pharmacological mechanism of geniposide in the treatment of atherosclerosis

A bibliometric analysis of vaccination against atherosclerosis

Jianpi Jiedu Recipe inhibits proliferation through reactive oxygen species-induced incomplete autophagy and reduces PD-L1 expression in colon cancer

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