Research progress on extraction, purification, structure and biological activity of Dendrobium officinale polysaccharides

He, Yuan; Li, Lin; Chang, Hao; Cai, Baochang; Gao, Huajun; Chen, Guoyu; Wen, Huiqing; Jappar, Zubaydan; Yan, Yizhe

doi:10.3389/fnut.2022.965073

Cited by 33 publications

(20 citation statements)

References 84 publications

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“…The monosaccharide composition is the most fundamental and core research object in plant polysaccharide research. It is closely related to the physicochemical properties, structural characteristics, and structure–activity relationship of polysaccharides [ 19 ]. In this study, the dry and fleshy stems of Cynomorium songaricum were used to isolate and purify CSP-III, whose molecular weight was determined to be 4,018,234 Da and the monosaccharide composition included Man, GlcA, GalA, Rha, Glc, Gal, and Ara at a molar ratio of 0.01:0.11:0.03:0.57:0.02:0.32:1.…”

Section: Discussionmentioning

confidence: 99%

Studies of the Immunomodulatory Activity of Polysaccharides from the Stem of Cynomorium songaricum Based on Intestinal Microbial Analysis

Lv,

Chen,

et al. 2023

Molecules

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Polysaccharides are the main effective components of Cynomorium songaricum’s stem that perform biological activities and have positive impacts on immune enhancement. In this study, the polysaccharide CSP-III of Cynomorium songaricum’s stem was isolated using a DEAE-52 cellulose column through Sephadex G-100 gel column chromatography. Upon analysis, the monosaccharide composition of CSP-III included Mannose (Man), Glucuronic acid (GlcA), Galacturonic acid (GalA), Rhamnose (Rha), Glucose (Glc), Galactose (Gal), and Arabinose (Ara), at a molar ratio of 0.01:0.11:0.03:0.57:0.02:0.32:1. The molecular weight of CSP-III was 4018234 Da. Meanwhile, the capacity of CSP-III, at various concentrations, to stimulate the proliferation of mouse spleen lymphocytes in vitro was compared, and the influence of CSP-III on cell proliferation was examined using RAW264.7 mouse mononuclear macrophages as a model. The influence of CSP-III on the expression of important phosphorylating proteins in the MAPK signaling pathway was initially analyzed by Western blotting. In RAW264.7 cells, CSP-III promoted the phosphorylation of JNK proteins, which thus activated the MAPK signaling cascade and exerted immunomodulatory effects. Moreover, according to in vivo studies using cyclophosphamide (CTX)-induced immunosuppression mouse models, CSP-III improved the CTX-induced histopathological damage, promoted T and B lymphocyte proliferation, upregulated CD4+ and CD8+ T-lymphocyte counts in the spleen, increased the serum levels of IgG and IgM, and activated three essential proteins of the MAPK signaling pathway. As revealed by analysis of intestinal flora, CSP-III improved the immune function by maintaining the homeostasis of the bacterial flora by boosting the relative abundances of some beneficial bacterial groups, such as Bacteroidetes, Desmodium, and Actinomyces, and reducing the relative abundance of Aspergillus phylum. Through in vitro and in vivo experiments, our present study demonstrates that polysaccharides from the stem of Cynomorium songaricum possess strong immunoregulatory effects. Findings in this work provide theoretical support for the potential application of Cynomorium songaricum in the field of health food.

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Section: Discussionmentioning

confidence: 99%

Studies of the Immunomodulatory Activity of Polysaccharides from the Stem of Cynomorium songaricum Based on Intestinal Microbial Analysis

Lv,

Chen,

et al. 2023

Molecules

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“…Dendrobium officinale Kimura et Migo ( D. officinale ) is an orchid plant with both medicinal and edible uses. D. officinale contains many bioactive components, including polysaccharides, polyphenols, flavonoids, and alkaloids, among which polysaccharides (DOPs) are the main one [ 24 , 25 , 26 ]. Zeng et al [ 27 ] reported that DOPs reduced H 2 O 2 -induced apoptosis in human gastric mucosal epithelial cells by decreasing ROS levels and improving cell nuclear morphology.…”

Section: Introductionmentioning

confidence: 99%

“…Historically, dendrobium polysaccharides have been prepared via hot water extraction, but it is an energy-intensive and time-consuming procedure. Therefore, ultrasonic, microwaves, acids, bases, and enzymes are used to assist extraction to improve extraction efficiency [ 26 ]. Enzyme-assisted extraction (EAE) is a gentler method than other extraction methods and is effective in breaking down cell walls and proteins to facilitate the release of polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

Insights into the Oxidative Stress Alleviation Potential of Enzymatically Prepared Dendrobium officinale Polysaccharides

et al. 2023

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(1) Background: The extraction parameters can dramatically alter the extraction rate and biological activity of polysaccharides. (2) Methods: Here, an enzyme-assisted extraction (EAE) was employed to extract D. officinale polysaccharides (DOPs), and its optimal extraction conditions were established by single-factor and Box–Behnken design (BBD) experiments. Further, on the basis of in vitro antioxidant capacity, the paraquat (PQ)-induced oxidative stress of Caenorhabditis elegans (C. elegans) was chosen as a research model to explore the antioxidant activity of DOPs. (3) Results: The results showed that the extraction yield of DOPs reached 48.66% ± 1.04% under the optimal condition. In vitro experiments had shown that DOPs have considerable ABTS+ radical scavenging capacity (EC50 = 7.27 mg/mL), hydroxyl radical scavenging capacity (EC50 = 1.61 mg/mL), and metal chelating power (EC50 = 8.31 mg/mL). Furthermore, in vivo experiments indicated that DOPs (0.25 mg/mL) significantly prolonged the lifespan, increased antioxidant enzyme activity, and upregulated the expression of daf-16 (>5.6-fold), skn-1 (>5.2-fold), and sir-2.1 (>2.3-fold) of C. elegans. (4) Conclusions: DOPs can be efficiently extracted by EAE and are effective in the reduction of oxidative stress levels in C. elegans.

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“…Tea is a drink made from the leaves or buds of the tea plant, which originated in China and has the longest history. There are numerous active ingredients in tea: tea polyphenols, tea polysaccharides, amino acids, alkaloids, vitamins, and so on [1]. Theophylline is not only one of the main flavor components in tea, but also has beneficial pharmacological effects.…”

Section: Introductionmentioning

confidence: 99%

A novel fluorescent aptasensor for the detection of theophylline based on cryonase‐driven signal amplification strategy

Feng,

Kang,

Yan

et al. 2023

Luminescence

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In the study, we have developed an expedient and efficient method for the detection of theophylline based on the amplification of the signal intensity of fluorescence based on oxidized single‐walled carbon nanohorns (oxSWCNHs)/cryonase. When theophylline was not present in the system, oxSWCNHs can adequately adsorb nucleic acid probes labeled by carboxyfluorescein (FAM). In the presence of theophylline, the nucleic acid probe forms the tertiary probe–theophylline complex, which detaches from the surface of the oxSWCNHs. Then, upon reaction with cryonase, the complex can release the FAM and theophylline into the next cycle. The fluorescence signal of the system exhibits a 1:N magnification, enabling quantitative detection of theophylline. The linear range was 30–150 ng/mL, and the limit of detection (LOD) was 6.04 ng/mL. At the same time, it can also be used to detect theophylline in mouse serum.

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Research progress on extraction, purification, structure and biological activity of Dendrobium officinale polysaccharides

Cited by 33 publications

References 84 publications

Studies of the Immunomodulatory Activity of Polysaccharides from the Stem of Cynomorium songaricum Based on Intestinal Microbial Analysis

Studies of the Immunomodulatory Activity of Polysaccharides from the Stem of Cynomorium songaricum Based on Intestinal Microbial Analysis

Insights into the Oxidative Stress Alleviation Potential of Enzymatically Prepared Dendrobium officinale Polysaccharides

A novel fluorescent aptasensor for the detection of theophylline based on cryonase‐driven signal amplification strategy

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