Research progress on chemopreventive effects of phytochemicals on colorectal cancer and their mechanisms

Yin, Tao; Wang, Min; Qing, Ying; Lin, Ying‐Min; Wu, Dong

doi:10.3748/wjg.v22.i31.7058

Cited by 47 publications

(40 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found that CYP24A1 expression was significantly decreased when added SFN to 1,25(OH)2D3, which is the opposite of what we hypothesized. However, in advanced Phytochemicals, such as sulforaphane, have been studied extensively for the treatment of various cancers in cell culture, animal models as well as humans (Appendix B; Yin, Wang, Qing, Lin, & Wu, 2016). They exhibit a wide range of safety and target multiple pathways in breast cancer cells (Schneider et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Histone deacetylase activity and vitamin D‐dependent gene expressions in relation to sulforaphane in human breast cancer cells

2019

View full text Add to dashboard Cite

Zhenhua Liu and Richard J. Wood are co-senior authors.Abbreviations: 125(OH)2D3, 1,25 dihydroxyvitamin D; BC, breast cancer; SEM, standard error of means; SFN, sulforaphane; TSA, trichostatin A. AbstractIt is relatively unknown how dietary bioactive compound, sulforaphane, in partnership with active vitamin D3, 1,25(OH)2D3, regulates vitamin D-dependent gene expression in breast cancer (BC). It has been suggested that the combination of various bioactive components with vitamins is crucial for their potential anticancer activities.METHODS: This study employed a combinatorial chemopreventive strategy to investigate the impact of dietary histone deacetylase (HDAC) inhibitor, that is, sulforaphane on chromatin remodeling in BC. To understand the epigenetics-mediated changes in gene expression, MCF-7 cells were exposed for 24 hr to 1,25(OH)2D3 (100nM) either alone or in combination with L-sulforaphane and TSA (20μM and 1μM, respectively) at 70% confluency. Changes in VDR, CYP24A1, CYP27B1, and TRPV6 gene expressions were quantified using real-time PCR-based assays. HDAC inhibitor activity was assessed using HDAC I/II assay that measured global changes in acetylation status.Cell viability was measured using ATP and MT assays. Clonogenic and migration assays were performed to analyze the ability of single cells to grow into colonies and % closure (migration ability) upon treatments, respectively. Results were expressed as ΔCT ± standard error of means (SEM) from One-way ANOVA analyses for mRNA expressions and mean ± SEM for all other assays. RESULTS: In MCF-7 cells, treatment with 1,25(OH)2D3 tended to decrease VDR (13 ± 0.4) and CYP27B1 (12 ± 0.96), while significantly increased TRPV6 (p = .02, 14 ± 0.1) and CYP24A1 (p < .0001, 0.38 ± 0.12) expression. D alone and D + TSA group had the opposite effects on HDAC inhibition from SFN alone, D + SFN, and TSA alone. The clonogenic assay showed a significant decrease in colony formation with no colonies for D + TSA (p < .03) and TSA alone groups (p < .03). Cell viability tended to decrease with D alone and in combination with TSA. CONCLUSION: These data suggest that the effects of 1,25(OH)2D3 and sulforaphane are selective and gene-specific in MCF-7 cells. Practical applicationsBreast cancer (BC) affects a large number of the U.S. population each year. Like most cancers, nutrition does play a role in the prevention of BC. However, dietary advice that includes reducing alcohol intake, red meat, and saturated fat consumption,

show abstract

Section: Discussionmentioning

confidence: 99%

Histone deacetylase activity and vitamin D‐dependent gene expressions in relation to sulforaphane in human breast cancer cells

2019

View full text Add to dashboard Cite

show abstract

“…Sulforaphane normally halts cell progression in the G0/G1 and G2/M phases, but can stop proliferation in G1/G2 [113]. …”

Section: Introductionmentioning

confidence: 99%

Microbial carcinogenic toxins and dietary anti-cancer protectants

Stone

Darlington

2017

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Several toxins are known which account for the ability of some bacteria to initiate or promote carcinogenesis. These ideas are summarised and evidence is discussed for more specific mechanisms involving chymotrypsin and the bacterial chymotryptic enzyme subtilisin. Subtilisin and Bacillus subtilis are present in the gut and environment and both are used commercially in agriculture, livestock rearing and meat processing. The enzymes deplete cells of tumour suppressors such as deleted in colorectal cancer (DCC) and neogenin, so their potential presence in the food chain might represent an important link between diet and cancer. Over-eating increases secretion of chymotrypsin which is absorbed from the gut and could contribute to several forms of cancer linked to obesity. Inhibition of these serine proteases by Bowman–Birk inhibitors in fruit and vegetables could account for some of the protective effects of a plant-rich diet. These interactions represent previously unknown non-genetic mechanisms for the modification of tumour suppressor proteins and provide a plausible explanation contributing to both the pro-oncogenic effects of meat products and the protective activity of a plant-rich diet. The data suggest that changes to farming husbandry and food processing methods to remove these sources of extrinsic proteases might significantly reduce the incidence of several cancers.

show abstract

“…However, the in vivo efficacy of the administered PEG, despite its low bioavailability, has led some researchers to interrogate whether metabolites would be the bioactive forms of the parent compound [59,60]. Nevertheless, some studies show less pharmaceutical impact of the metabolites and indicate a decrease in the expression of SULT enzymes in certain types of cancer cells, which indicates an overall low sulfation activity in these cells comparing to the normal ones.…”

Section: Metabolismmentioning

confidence: 99%

“…RSV undergoes extensive phase I (oxidation, reduction, and hydrolysis) and phase II (glucuronic acid, sulfate, and methyl conjugations) biochemical changes immediately after ingestion [60,61], being metabolized into both glucuronic acid and sulfate conjugations of the phenolic groups in liver and intestinal epithelial cells [60][61][62][63]. Hydrogenation of the aliphatic double bond is also present [64].…”

Section: Metabolismmentioning

confidence: 99%

“…In humans, about 70% of orally administered RSV (25 mg) is rapidly (<30 min) absorbed and metabolized with a peak plasma level of ≈ 2 μM of RSV metabolites and a half-life of 9-10 h [57][58][59][60]. The extent to which the human colon can absorb and metabolize RSV depends on the metabolic activity and on the hepatic function of the local intestinal microflora.…”

Section: Absorption and Bioavailabilitymentioning

confidence: 99%

See 1 more Smart Citation

Reduction of Hepatotoxicity Induced by Doxorubicin

Bengaied¹,

Ribeiro²,

Amri³

et al. 2017

J Integr Oncol

View full text Add to dashboard Cite

Doxorubicin (DOX) has been used in the treatment of variety of cancers but its administration is limited by a dosedependent toxicity. Its cytotoxic effects on malignant cells have shown an increase in the risk of cardiotoxicity, hepatoxicity, renal insufisance.Antioxydants have been explored for both their cancer preventive properties and chemodulatory of DOX toxicity. Resveratrol (RSV) is a polyphenolic constituent of several dietary mainly of grapes and wine origin recently its anticancer potential has been extensively explored, revealing its anti-proliferative effect on different cancer cell lines, both in vitro and in vivo. RSV is also known to have modulatory effects on cell apoptosis, migration and growth via various signaling pathways. Though, RSV possesses great medicinal value, its applications as a therapeutic drug is limited. Problems like low oral bioavailability and poor aqueous solubility make RSV an unreliable candidate for therapeutic purposes. Additionally, the rapid gastrointestinal digestion of RSV is also a major barrier for its clinical translation. Hence, to overcome these disadvantages RSV-based nanodelivery systems have been considered in recent times. Nanodelivery systems of RSV have shown promising results in its uptake by the epithelial system as well as enhanced delivery to the target site. Herein we have tried to bring new new insights into the molecular mechanisms of DOX toxicity with respect to DNA damage, free radicals and whether RSV can be a playmaker as chemodulatory of DO x .

show abstract

Research progress on chemopreventive effects of phytochemicals on colorectal cancer and their mechanisms

Cited by 47 publications

References 98 publications

Histone deacetylase activity and vitamin D‐dependent gene expressions in relation to sulforaphane in human breast cancer cells

Histone deacetylase activity and vitamin D‐dependent gene expressions in relation to sulforaphane in human breast cancer cells

Microbial carcinogenic toxins and dietary anti-cancer protectants

Reduction of Hepatotoxicity Induced by Doxorubicin

Contact Info

Product

Resources

About