Research Progress of Platelet Transfusion in China

Wang, Qiushi; Yang, Jiangcun; Stevens, Lori; Wang, Deqing

doi:10.1016/j.tmrv.2016.11.005

Cited by 8 publications

(6 citation statements)

References 21 publications

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“…The expression of CD36 antigen has been shown to vary according to geographic region (Table 4, Table 5). In the present study, the frequency of CD36 deficiency was estimated to be 2.64% in 13,778 cases of individuals in China, significantly lower than reported in other Asian countries [[10], [13]]. In addition, the frequency of type I CD36 deficiency was significantly lower in northern regions than in southern regions, with no difference in the frequency of type II CD36 deficiency observed between regions.…”

Section: Discussioncontrasting

confidence: 68%

High-Throughput CD36 Phenotyping on Human Platelets Based on Sandwich ELISA and Mutant Gene Analysis

He¹,

Tang²,

Jin³

et al. 2023

Transfus Med Hemother

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Background: CD36 deficiency is closely associated with fetal/neonatal alloimmune thrombocytopenia, platelet transfusion refractoriness, and other hemorrhage disorders, particularly in Asian and African populations. There is a clinical need for rapid and high-throughput methods of platelet CD36 (pCD36) phenotyping to improve the availability of CD36 typing of donors and assist clinical blood transfusions for patients with anti-CD36 antibodies. Such methods can also support the establishment of databases of pCD36-negative phenotypes. Study Design and Methods: A sandwich enzyme-linked immunosorbent assay (ELISA) for CD36 phenotyping of human platelets was developed using anti-CD36 monoclonal antibodies. The reliability of the assay was evaluated by calculating the intra-assay and inter-assay coefficients of variation (CV). A total of 1,691 anticoagulant whole blood samples from healthy blood donors were randomly selected. PCD36 expression was measured using a sandwich ELISA. PCD36 deficiency was confirmed by flow cytometry (FC). Mutations underlying pCD36 deficiency were identified using polymerase chain reaction sequence-based typing (PCR-SBT). Results: The sandwich ELISA for pCD36 phenotyping had high reliability (intra-assay CV, 2.1–4.8%; inter-assay CV, 2.3–5.2%). The sandwich ELISA was used to screen for CD36 expression on platelets isolated from 1,691 healthy blood donors. Of these, 36 samples were pCD36-negative. FC demonstrated absence of CD36 expression on monocytes in three of the 36 cases. In the present study population, the frequency of CD36 deficiency was 2.13% (36/1,691), of which 0.18% (3/1,691) was type I deficiency and 1.95% (33/1,691) was type II deficiency. In addition, we used PCR-SBT to characterize the gene mutations in exons 3–14 of the CD36 gene in 27 cases of CD36 deficiency and discovered 10 types of mutations in 13 pCD36-negative samples. Conclusion: The present study describes the development and characterization of a highly reliable sandwich ELISA for high-throughput screening for pCD36 expression. This novel method is feasible for clinical applications and provides a useful tool for the establishment of databases of pCD36-negative phenotype donors.

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Section: Discussioncontrasting

confidence: 68%

High-Throughput CD36 Phenotyping on Human Platelets Based on Sandwich ELISA and Mutant Gene Analysis

He¹,

Tang²,

Jin³

et al. 2023

Transfus Med Hemother

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“…Platelet transfusion is generally used either prophylactically to reduce the risk of bleeding or therapeutically to control active bleeding in many clinical settings . Liquid‐preserved platelets kept under standard blood banking conditions, at 20 to 24 °C with constant agitation, can be stored for only 5 to 7 days, which severely limits their availability at far‐forward combat facilities and remote civilian locations …”

mentioning

confidence: 99%

Cryopreserved platelets augment the inflammatory response: role of phosphatidylserine‐ and P‐selectin–mediated platelet phagocytosis in macrophages

Zhao

Gan

et al. 2019

Transfusion

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BACKGROUND Cryopreservation in dimethyl sulfoxide and storage at −80 °C extends the shelf life of platelets to at least 2 years, allowing greater availability in rural and military areas. While cryopreserved platelets (CPPs) have been extensively characterized for coagulation and thrombin generation, reports on the mechanism of adverse reactions to CPPs transfusion are scarce. Here, we tested the hypothesis that CPPs facilitate phagocytosis by Kupffer cells and subsequently promote the inflammatory response in Kupffer cells. STUDY DESIGN AND METHODS P‐selectin expression, glycoprotein Ibα clustering and phosphatidylserine (PS) surface exposure on platelets stored at 22 °C, 4 °C and − 80 °C for 3 days were examined by flow cytometry. The phagocytosis of mepacrine‐labeled platelets coincubated with THP‐1 cells was examined by flow cytometry and confocal microscopy, and the release of cytokines from THP‐1 cells was measured by enzyme‐linked immunosorbent assay. RESULTS CPPs showed a marked enhancement of exposed PS but dramatically reduced glycoprotein Ibα expression and clustering compared with platelets stored at 4 °C. Activation of THP‐1 cells was stronger by CPPs than by platelets stored at 22 °C and 4 °C. CPP interference tests using annexin V and anti–P‐selectin showed that CPPs induced increases in PS‐ and P‐selectin–mediated phagocytosis, as well as secretion of the proinflammatory cytokine tumor necrosis factor‐α, and interleukins IL‐1β and IL‐6, but a decrease in transforming growth factor‐β production in THP‐1 cells. Surface‐exposed PS was more effective than P‐selectin for the activation of THP‐1 cells. CONCLUSION CPPs triggered PS and P‐selectin–mediated phagocytosis by macrophages and stimulated the inflammatory response of macrophages.

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“…Platelets were obtained by separating whole blood from Spectra Optia Blood Cell Separator (Carlsbad, CA, USA), and the number of platelets in each bag was ≥2.5×10 11 /L, and were transfused within 24 hours on the day of collection or stored at 4°C. Unrelated platelets were provided by Jinhua Blood Center and met the requirements of Chinese quality standards 11 . Each bag platelets is 9-12 units, and the number of platelets is ≥2.5×10 11 /L.…”

Section: Platelet Products and Transfusion Policymentioning

confidence: 99%

Hemostatic Effect of Related Donor Platelet Transfusion: A Retrospective Cohort Study

zhang,

Ni,

et al. 2022

SSRN Journal

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Research Progress of Platelet Transfusion in China

Cited by 8 publications

References 21 publications

High-Throughput CD36 Phenotyping on Human Platelets Based on Sandwich ELISA and Mutant Gene Analysis

High-Throughput CD36 Phenotyping on Human Platelets Based on Sandwich ELISA and Mutant Gene Analysis

Cryopreserved platelets augment the inflammatory response: role of phosphatidylserine‐ and P‐selectin–mediated platelet phagocytosis in macrophages

Hemostatic Effect of Related Donor Platelet Transfusion: A Retrospective Cohort Study

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