Research progress of biosensors for detection of SARS-CoV-2 variants based on ACE2

Wei, Hongshuai; Zhang, Congcong; Du, Xin; Zhang, Zhenguo

doi:10.1016/j.talanta.2022.123813

Cited by 20 publications

(14 citation statements)

References 106 publications

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“…Here, we present a gFET sensor that exploits as bioreceptor a chimeric version of the human receptor ACE2 which outperforms an anti-Spike antibody in detecting specifically the SARS-CoV-2 Spike protein. Recently, other ACE2-based sensors have been developed [81] (Table S4), but none of them are optimized to resemble its physiological counterpart and none of them tested all the known variants. We believe that using a chimeric version of ACE2 composed of the soluble part of the protein and the Fc region of the antibodies is a better bioreceptor choice to detect SARS-CoV-2.…”

Section: Discussionmentioning

confidence: 99%

SARS-CoV-2 multi-variant rapid detector based on graphene transistor functionalized with an engineered dimeric ACE2 receptor

et al. 2023

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Section: Discussionmentioning

confidence: 99%

SARS-CoV-2 multi-variant rapid detector based on graphene transistor functionalized with an engineered dimeric ACE2 receptor

et al. 2023

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“…35,36 Moreover, studies show that the affinity of ACE2 for the S protein of SARS-CoV-2 increases with viral mutation, [37][38][39][40] and therefore ACE2 has the potential to be used as a recognition element in different platforms such as electrochemical sensors for the detection of SARS-CoV-2 and mutant strains of SARS-CoV-2. [41][42][43][44][45][46]…”

Section: Intact Virus Particle Detectionmentioning

confidence: 99%

Point-of-care testing of infectious diseases: recent advances

Shang

Guo

2023

Sens. Diagn.

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“…The existing detection tool for ACE2 expression in tumors and other pathological processes included but not limited to western blot (WB), immunohistochemistry (IHC), polymerase chain reaction (PCR), and enzyme-linked immunosorbent assay (ELISA) [ 10 ], all of which cannot elucidate the systematic ACE2 fluctuation as a whole and cannot be visualized in vivo, in particular, lacking the ability of dynamic and quantitative assessment of whole body. In consideration of the sensitivity of PET imaging and specificity of molecular imaging, ACE2 PET may be meaningful in the detection, localization, and functional evaluation for multiple tumors, especially when combined with CT or MRI; meanwhile, ACE2 PET is promising to differentiate the tumors between subtypes, and determine the primary foci and metastasis.…”

Section: Introductionmentioning

confidence: 99%

68Ga-cyc-DX600 PET/CT in ACE2-targeted tumor imaging

Ren

Jiang

Shi

et al. 2023

Eur J Nucl Med Mol Imaging

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Purpose For the tumor-specific ACE2 expression, this research aimed to establish and verify ACE2-targeted PET imaging in differentiating tumors with distinct ACE2 expression. Methods 68 Ga-cyc-DX600 was synthesized as tracer of ACE2 PET. NOD-SCID mice were used to prepare the subcutaneous tumor models with HEK-293 or HEK-293T/hACE2 cells to verify ACE2 specificity, with other kinds of tumor cells to evaluate the diagnostic efficiency for ACE2 expression, additionally, immunohistochemical analysis and western blot were used to certify the findings on ACE2 PET, which was then performed on four cancer patients and compared with FDG PET. Results The metabolic clearance of 68 Ga-cyc-DX600 was initially completed in 60 min, realizing an ACE2-dependent and organ-specific background of ACE2 PET; meanwhile, tracer uptake of subcutaneous tumor models was of a definite dependence on ACE2 expression ( r = 0.903, p < 0.05), and the latter served as the primary factor when ACE2 PET was used for the differential diagnosis of ACE2-related tumors. In pre-clinical practice, a comparable tumor-to-background ratio was acquired in ACE2 PET of a lung cancer patient at 50 and 80 min post injection; the quantitative values of ACE2 PET and FDG PET were negatively correlated ( r = − 0.971 for SUV max , p = 0.006; r = − 0.994 for SUV mean , p = 0.001) in an esophageal cancer patient, no matter the primary lesion or metastasis. Conclusions 68 Ga-cyc-DX600 PET was an ACE2-specific imaging for the differential diagnosis of tumors and added complementary value to conventional nuclear medicine diagnosis, such as FDG PET on glycometabolism.

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Research progress of biosensors for detection of SARS-CoV-2 variants based on ACE2

Cited by 20 publications

References 106 publications

SARS-CoV-2 multi-variant rapid detector based on graphene transistor functionalized with an engineered dimeric ACE2 receptor

SARS-CoV-2 multi-variant rapid detector based on graphene transistor functionalized with an engineered dimeric ACE2 receptor

Point-of-care testing of infectious diseases: recent advances

68Ga-cyc-DX600 PET/CT in ACE2-targeted tumor imaging

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