Research progress in the development of porcine reproductive and respiratory syndrome virus as a viral vector for foreign gene expression and delivery

Dai, Guo; Huang, Mei; Fung, To Sing; Liu, Ding Xiang

doi:10.1080/14760584.2020.1857737

Cited by 7 publications

(6 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a highly diverse and variable virus, it's possible to engineer the PRRSV genome with point mutations, insertions, deletions, replacements, or recombinations [ 29 ]. The nsp2 coding region is regarded as the most hypervariable region of the PRRSV genome, and many viruses with modifications in this region have been successfully rescued.…”

Section: Discussionmentioning

confidence: 99%

A novel strategy to attenuate porcine reproductive and respiratory syndrome virus by inhibiting viral replication in the target pulmonary alveolar macrophages via hematopoietic-specific miR-142

Tan

et al. 2023

One Health Adv.

View full text Add to dashboard Cite

Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically important pathogen for the global pork industry. Although modified live virus (MLV) vaccines are commonly used for PRRSV prevention and control, they still carry a risk of infecting the host and replicating in target cells, thereby increasing the likehood of virus recombination and reversion to virulence. In this study, we inserted the target sequence of miR-142 into the nsp2 hypervariable region of PRRSV to inhibit viral replication in its host cells of pigs, with the aim of achieving virus attenuation. The chimeric virus RvJX-miR-142t was successfully rescued and retained its growth characteristics in MARC-145 cells. Furthermore, it did not replicate in MARC-145 cells transfected with miRNA-142 mimic. We also observed limited replication ability of RvJX-miR-142t in pulmonary alveolar macrophages, which are the main cell types that PRRSV infects. Our animal inoculation study showed that pigs infected with RvJX-miR-142t displayed less severe clinical symptoms, lower viremia titers, lighter lung lesions, and significantly lower mortality rates during the first 7 days post-inoculation, in comparison to pigs infected with the backbone virus RvJXwn. We detected a partially deletion of the miR-142 target sequence in the RvJX-miR-142t genome at 14 dpi. It is highly possible that the reversion of viral virulence observed in the later timepoints of our animal experiment was caused by that. Our study provided a new strategy for attenuating PRRSV and confirmed its effectiveness. However, further studies are necessary to increase the stability of this virus under host selection pressure.

show abstract

Section: Discussionmentioning

confidence: 99%

A novel strategy to attenuate porcine reproductive and respiratory syndrome virus by inhibiting viral replication in the target pulmonary alveolar macrophages via hematopoietic-specific miR-142

Tan

et al. 2023

One Health Adv.

View full text Add to dashboard Cite

show abstract

“…The PRRSV has brought huge losses to the pork industry in China and even the world. Although the resistance of pigs can be improved through vaccine research and development, it is difficult to control the PRRSV epidemic simply by using vaccines due to the strong variability of the PRRSV [ 21 ]. Therefore, PRRSV resistance genetic breeding has become an important way to control PRRSV infection.…”

Section: Discussionmentioning

confidence: 99%

Signaling Lymphocytic Activation Molecule Family Member 1 Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication

Song

Jima

et al. 2022

Animals

View full text Add to dashboard Cite

The porcine reproductive and respiratory syndrome virus (PRRSV) causes a highly contagious disease in domestic swine. Signaling lymphocytic activation molecule family member 1 (SLAMF1) is a costimulatory factor that is involved in innate immunity, inflammation, and infection. Here, we demonstrate that overexpression of the SLAMF1 gene inhibited PRRSV replication significantly and reduced the levels of key signaling pathways, including MyD88, RIG-I, TLR2, TRIF, and inflammatory factors IL-6, IL-1β, IL-8, TNF-β, TNF-α, and IFN-α in vitro. However, the knockdown of the SLAMF1 gene could enhance replication of the PRRSV and the levels of key signaling pathways and inflammatory factors. Overall, our results identify a new, to our knowledge, antagonist of the PRRSV, as well as a novel antagonistic mechanism evolved by inhibiting innate immunity and inflammation, providing a new reference and direction for PRRSV disease resistance breeding.

show abstract

“…Currently, each of these three diseases are vaccinated against individually, which is costly. This tri-valent vaccine was shown to confer protection against all three viruses [ 95 ], although the genetic stability of PRRSV vectors needs to be improved [ 95 , 97 ]. Nevertheless, this shows potential for the use of PRRSV as a viral vector.…”

Section: Immunising Pigs With Bi/multivalent Vaccines Could Help Prev...mentioning

confidence: 99%

The pig as an amplifying host for new and emerging zoonotic viruses

McLean

Graham

2022

One Health

View full text Add to dashboard Cite

Research progress in the development of porcine reproductive and respiratory syndrome virus as a viral vector for foreign gene expression and delivery

Abstract: Research progress in the development of porcine reproductive and respiratory syndrome virus as a viral vector for foreign gene expression and delivery

Cited by 7 publications

References 66 publications

A novel strategy to attenuate porcine reproductive and respiratory syndrome virus by inhibiting viral replication in the target pulmonary alveolar macrophages via hematopoietic-specific miR-142

A novel strategy to attenuate porcine reproductive and respiratory syndrome virus by inhibiting viral replication in the target pulmonary alveolar macrophages via hematopoietic-specific miR-142

Signaling Lymphocytic Activation Molecule Family Member 1 Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication

The pig as an amplifying host for new and emerging zoonotic viruses

Contact Info

Product

Resources

About