Research Progress in RNA-Binding Proteins

Smirnov, Alexandre

doi:10.3390/ijms24010058

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…Understanding the specific roles of these RBPs in adipose metabolism could provide valuable insights into the regulation of adipose tissue and potentially lead to new therapeutic targets for metabolic disorders such as obesity and diabetes. Discussion: RNA-binding proteins (RBPs) play a crucial role in the modulation of gene expression and possess the ability to impact the stability and functionality of RNA molecules 13,14,49 . Moreover, the involvement of RNA-binding proteins in the pathogenesis and advancement of various diseases, including cancer, neurodegenerative disorders, and viral infections, has been well documented 14,16,17,[50][51][52][53][54] .…”

Section: Rbps Of Interestmentioning

confidence: 99%

Expression landscape of RNA-binding proteins (RBPs) during adipogenesis and diet-induced obesity

Dairi,

Alswaji,

Alzahrani

et al. 2023

Preprint

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RNA-binding proteins (RBPs) are key regulators of various cellular processes, including mRNA splicing, transport, stability, and translation. They play a crucial role in post-transcriptional gene regulation by interacting with specific RNA sequences or structures. RBPs can also influence the localization of mRNAs within the cell and participate in the formation of RNA-protein complexes that control gene expression. Dysregulation or mutations in RBPs have been associated with a wide range of diseases, including neurodegenerative disorders, cancer, and developmental abnormalities. Recent studies have highlighted the importance of RBPs in regulating adipocyte differentiation, lipid metabolism, and insulin sensitivity. However, the global expression patterns and functional contributions of RBPs during adipogenesis and in regulating adipocyte functioning remain poorly understood. Identifying and characterizing RBPs during adipogenesis could provide valuable insights into the molecular mechanisms underlying adipocyte differentiation and potentially lead to the development of novel therapeutic strategies for obesity-related disorders. Using a combination of bioinformatics tools and experimental validation, we identified a set of novel RBPs that may have important roles in adipogenesis and adipocyte functioning. We analyzed the expression of the entire repertoire of RBPs during the adipogenic differentiation of murine adipocyte cell line, 3T3-L1 and assessed the expression of RBPs in stromal vesicular fraction (SVF) and adipocyte fraction of mouse epididymal adipose tissue. We also evaluated the impact of high fat diet on the expression of RBPs in adipocytes isolated from mouse epididymal adipose tissue. We found a number of RBPS that were significantly up- or down-regulated in 3T3-L1 cells during adipogenic differentiation and were enriched in the stromal vascular fraction (SVF) or adipocyte fraction of mouse adipose tissue. In addition, they exhibited differential expression in adipocytes isolated from diet-induced obese mice compared to control mice. The majority of these RBPs have no known role in adipogenesis or adipose metabolism. These proteins could potentially play a novel role in adipogenic differentiation and adipose metabolism, suggesting the presence of previously unknown regulatory mechanisms. Further investigation is needed to understand the specific functions of these RBPs and their potential impact on adipose tissue development and metabolism. This discovery opens up new avenues for research in uncovering the intricate regulatory networks involved in adipogenesis and adipose tissue homeostasis.

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Section: Rbps Of Interestmentioning

confidence: 99%

Expression landscape of RNA-binding proteins (RBPs) during adipogenesis and diet-induced obesity

Dairi,

Alswaji,

Alzahrani

et al. 2023

Preprint

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RNA binding proteins in senescence: A potential common linker for age-related diseases?

Varesi

Campagnoli

Barbieri

et al. 2023

Ageing Research Reviews

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RNA-binding protein transcripts as potential biomarkers for detecting Primary Sclerosing Cholangitis and for predicting its progression to Cholangiocarcinoma

Ala,

Fagoonee

2024

Front. Mol. Biosci.

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Primary Sclerosing Cholangitis (PSC) is a persistent inflammatory liver condition that affects the bile ducts and is commonly diagnosed in young individuals. Despite efforts to incorporate various clinical, biochemical and molecular parameters for diagnosing PSC, it remains challenging, and no biomarkers characteristic of the disease have been identified hitherto. PSC is linked with an uncertain prognosis, and there is a pressing need to explore multiomics databases to establish a new biomarker panel for the early detection of PSC’s gradual progression into Cholangiocarcinoma (CCA) and for the development of effective therapeutic interventions. Apart from non-coding RNAs, other components of the Ribonucleoprotein (RNP) complex, such as RNA-Binding Proteins (RBPs), also hold great promise as biomarkers due to their versatile expression in pathological conditions. In the present review, an update on the RBP transcripts that show dysregulated expression in PSC and CCA is provided. Moreover, by utilizing a bioinformatic data mining approach, we give insight into those RBP transcripts that also exhibit differential expression in liver and gall bladder, as well as in body fluids, and are promising as biomarkers for diagnosing and predicting the prognosis of PSC. Expression data were bioinformatically extracted from public repositories usingTCGA Bile Duct Cancer dataset for CCA and specific NCBI GEO datasets for both PSC and CCA; more specifically, RBPs annotations were obtained from RBP World database. Interestingly, our comprehensive analysis shows an elevated expression of the non-canonical RBPs, FANCD2, as well as the microtubule dynamics regulator, ASPM, transcripts in the body fluids of patients with PSC and CCA compared with their respective controls, with the same trend in expression being observed in gall bladder and liver cancer tissues. Consequently, the manipulation of tissue expression of RBP transcripts might be considered as a strategy to mitigate the onset of CCA in PSC patients, and warrants further experimental investigation. The analysis performed herein may be helpful in the identification of non-invasive biomarkers for the early detection of PSC and for predicting its progression into CCA. In conclusion, future clinical research should investigate in more depth the full potential of RBP transcripts as biomarkers for human pathologies.

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Research Progress in RNA-Binding Proteins

Abstract: RNA-binding proteins are everywhere and accompany RNA molecules at every stage of their molecular life, from “birth” (transcription) through “growing up” (maturation), “active life” (molecular function) until “death” (turnover) [...]

Cited by 3 publications

References 17 publications

Expression landscape of RNA-binding proteins (RBPs) during adipogenesis and diet-induced obesity

Expression landscape of RNA-binding proteins (RBPs) during adipogenesis and diet-induced obesity

RNA binding proteins in senescence: A potential common linker for age-related diseases?

RNA-binding protein transcripts as potential biomarkers for detecting Primary Sclerosing Cholangitis and for predicting its progression to Cholangiocarcinoma

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