Research Progress in Digging and Validation of miRNA Target Genes Using Experimental Methods

Luo, Xiangdong; Bai, Xi; Cai, Hua; Ji, Wei; Liu, Xin; Tang, Lili; Zhu, Yanming

doi:10.1016/s1006-8104(13)60043-4

Cited by 1 publication

(1 citation statement)

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“…New protocols for optimizing PCR amplification and other techniques can increase throughput without requiring high DNA input, although to limit bias more thoroughly one would have to avoid library amplification by PCR (Aird et al ., ). False positives in target identification can also be remedied through creating and standardizing new computational protocols and criteria (Kuhn et al ., ; Soneson and Delorenzi, ; Xiao et al ., ).…”

Section: The Future Impact Of Manipulating Mirnas In Biofuel Feedstocksmentioning

confidence: 97%

Manipulating microRNAs for improved biomass and biofuels from plant feedstocks

Trumbo

Zhang

Stewart

2015

Plant Biotechnology Journal

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SummaryPetroleum-based fuels are nonrenewable and unsustainable. Renewable sources of energy, such as lignocellulosic biofuels and plant metabolite-based drop-in fuels, can offset fossil fuel use and reverse environmental degradation through carbon sequestration. Despite these benefits, the lignocellulosic biofuels industry still faces many challenges, including the availability of economically viable crop plants. Cell wall recalcitrance is a major economic barrier for lignocellulosic biofuels production from biomass crops. Sustainability and biomass yield are two additional, yet interrelated, foci for biomass crop improvement. Many scientists are searching for solutions to these problems within biomass crop genomes. MicroRNAs (miRNAs) are involved in almost all biological and metabolic process in plants including plant development, cell wall biosynthesis and plant stress responses. Because of the broad functions of their targets (e.g. auxin response factors), the alteration of plant miRNA expression often results in pleiotropic effects. A specific miRNA usually regulates a biologically relevant bioenergy trait. For example, relatively low miR156 overexpression leads to a transgenic feedstock with enhanced biomass and decreased recalcitrance. miRNAs have been overexpressed in dedicated bioenergy feedstocks such as poplar and switchgrass yielding promising results for lignin reduction, increased plant biomass, the timing of flowering and response to harsh environments. In this review, we present the status of miRNA-related research in several major biofuel crops and relevant model plants. We critically assess published research and suggest next steps for miRNA manipulation in feedstocks for increased biomass and sustainability for biofuels and bioproducts.

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Section: The Future Impact Of Manipulating Mirnas In Biofuel Feedstocksmentioning

confidence: 97%