Research progress in decellularized extracellular matrix-derived hydrogels

Zhang, Wenhui; Du, Aoling; Liu, Shun; Lv, Mingyue; Chen, Shenghua

doi:10.1016/j.reth.2021.04.002

Cited by 83 publications

(61 citation statements)

References 125 publications

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“…With regard to the brain, ECMs have been extracted and their compositions have been compared with the native tissue and Matrigel [180] or between different brain regions [181] using mass spectrometry; however, no endothelial studies have been performed with those yet. Recently, the use of organ-specific decellularized ECM hydrogels [182] and decellularized ECM scaffolds [183] have been reviewed elsewhere.…”

Section: Organ-specific Responses To Specific Biochemical Cuesmentioning

confidence: 99%

Organ-Specific Endothelial Cell Differentiation and Impact of Microenvironmental Cues on Endothelial Heterogeneity

Gifre-Renom

Daems

Luttun

et al. 2022

IJMS

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Endothelial cells throughout the body are heterogeneous, and this is tightly linked to the specific functions of organs and tissues. Heterogeneity is already determined from development onwards and ranges from arterial/venous specification to microvascular fate determination in organ-specific differentiation. Acknowledging the different phenotypes of endothelial cells and the implications of this diversity is key for the development of more specialized tissue engineering and vascular repair approaches. However, although novel technologies in transcriptomics and proteomics are facilitating the unraveling of vascular bed-specific endothelial cell signatures, still much research is based on the use of insufficiently specialized endothelial cells. Endothelial cells are not only heterogeneous, but their specialized phenotypes are also dynamic and adapt to changes in their microenvironment. During the last decades, strong collaborations between molecular biology, mechanobiology, and computational disciplines have led to a better understanding of how endothelial cells are modulated by their mechanical and biochemical contexts. Yet, because of the use of insufficiently specialized endothelial cells, there is still a huge lack of knowledge in how tissue-specific biomechanical factors determine organ-specific phenotypes. With this review, we want to put the focus on how organ-specific endothelial cell signatures are determined from development onwards and conditioned by their microenvironments during adulthood. We discuss the latest research performed on endothelial cells, pointing out the important implications of mimicking tissue-specific biomechanical cues in culture.

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Section: Organ-specific Responses To Specific Biochemical Cuesmentioning

confidence: 99%

Organ-Specific Endothelial Cell Differentiation and Impact of Microenvironmental Cues on Endothelial Heterogeneity

Gifre-Renom

Daems

Luttun

et al. 2022

IJMS

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“…Therefore, the uniformization of methodologies concerning CRC tissue decellularization would be essential for the reproducibility of results. Decellularized ECM can be cell-, animal-, or human- or patient-derived and have been applied as scaffolds for 3D cell cultures [ 145 ], bioprinting techniques for creating adaptable 3D structures, and as components in synthetic and natural cell culture platforms [ 146 ], providing bioactive ECM components in tunable constructs [ 147 ]. For example, the use of porcine jejunum decellularized matrix, derived from the patented Biological Vascularized Scaffold (DE:302014007893; BioVaSc ® , proprietary knowhow Fraunhofer IGB) [ 148 ], is an interesting 3D in vitro colon model.…”

Section: Decellularized Colorectal Cancer Matrices As Bioactive Scaffolds For Modeling the Tumor Microenvironmentmentioning

confidence: 99%

Decellularized Colorectal Cancer Matrices as Bioactive Scaffolds for Studying Tumor-Stroma Interactions

Marques-Magalhães

Cruz

Costa

et al. 2022

Cancers

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More than a physical structure providing support to tissues, the extracellular matrix (ECM) is a complex and dynamic network of macromolecules that modulates the behavior of both cancer cells and associated stromal cells of the tumor microenvironment (TME). Over the last few years, several efforts have been made to develop new models that accurately mimic the interconnections within the TME and specifically the biomechanical and biomolecular complexity of the tumor ECM. Particularly in colorectal cancer, the ECM is highly remodeled and disorganized and constitutes a key component that affects cancer hallmarks, such as cell differentiation, proliferation, angiogenesis, invasion and metastasis. Therefore, several scaffolds produced from natural and/or synthetic polymers and ceramics have been used in 3D biomimetic strategies for colorectal cancer research. Nevertheless, decellularized ECM from colorectal tumors is a unique model that offers the maintenance of native ECM architecture and molecular composition. This review will focus on innovative and advanced 3D-based models of decellularized ECM as high-throughput strategies in colorectal cancer research that potentially fill some of the gaps between in vitro 2D and in vivo models. Our aim is to highlight the need for strategies that accurately mimic the TME for precision medicine and for studying the pathophysiology of the disease.

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“…Decellularized Extracellular Matrix (dECM) Decellularized ECM (dECM) has gained more popularity in using it as hydrogels for tissue regeneration, in particular for organoid formation in vitro [34]. This complex network of macromolecular substances has a crucial role in cell adhesion, migration, differentiation, and functional expression regulating tissue development and homeostasis [35]. Matrigel ® is one of the commercial and most common ECM derived from mouse tumors, which secrete abundant basement membrane proteins such as laminin, collagen IV, entactin, heparan sulfate, etc.…”

Section: Lamininmentioning

confidence: 99%

“…dECM has been isolated from different tissue sources, including human, porcine, bovine, mouse among others, by mechanical, chemical and/or enzymatical process [37,38]. Generally, the dECM gels can be formed by temperature, salt ion concentration, and pH change or by the addition of crosslinking agents [35].…”

Section: Lamininmentioning

confidence: 99%

Polymeric Scaffolds for Dental, Oral, and Craniofacial Regenerative Medicine

Munguia-Lopez

Cho

et al. 2021

Molecules

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Dental, oral, and craniofacial (DOC) regenerative medicine aims to repair or regenerate DOC tissues including teeth, dental pulp, periodontal tissues, salivary gland, temporomandibular joint (TMJ), hard (bone, cartilage), and soft (muscle, nerve, skin) tissues of the craniofacial complex. Polymeric materials have a broad range of applications in biomedical engineering and regenerative medicine functioning as tissue engineering scaffolds, carriers for cell-based therapies, and biomedical devices for delivery of drugs and biologics. The focus of this review is to discuss the properties and clinical indications of polymeric scaffold materials and extracellular matrix technologies for DOC regenerative medicine. More specifically, this review outlines the key properties, advantages and drawbacks of natural polymers including alginate, cellulose, chitosan, silk, collagen, gelatin, fibrin, laminin, decellularized extracellular matrix, and hyaluronic acid, as well as synthetic polymers including polylactic acid (PLA), polyglycolic acid (PGA), polycaprolactone (PCL), poly (ethylene glycol) (PEG), and Zwitterionic polymers. This review highlights key clinical applications of polymeric scaffolding materials to repair and/or regenerate various DOC tissues. Particularly, polymeric materials used in clinical procedures are discussed including alveolar ridge preservation, vertical and horizontal ridge augmentation, maxillary sinus augmentation, TMJ reconstruction, periodontal regeneration, periodontal/peri-implant plastic surgery, regenerative endodontics. In addition, polymeric scaffolds application in whole tooth and salivary gland regeneration are discussed.

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Research progress in decellularized extracellular matrix-derived hydrogels

Cited by 83 publications

References 125 publications

Organ-Specific Endothelial Cell Differentiation and Impact of Microenvironmental Cues on Endothelial Heterogeneity

Organ-Specific Endothelial Cell Differentiation and Impact of Microenvironmental Cues on Endothelial Heterogeneity

Decellularized Colorectal Cancer Matrices as Bioactive Scaffolds for Studying Tumor-Stroma Interactions

Polymeric Scaffolds for Dental, Oral, and Craniofacial Regenerative Medicine

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