Research progress and strategies for multifunctional rapeseed: A case study of China

Fu, Donghui; Jiang, Lingyan; Mason, Annaliese S.; Xiao, Meili; Zhu, Long-rong; Li-zhi, LI; Zhou, Qinghong; Shen, Chang-jian; Huang, Chunhui

doi:10.1016/s2095-3119(16)61384-9

Cited by 77 publications

(53 citation statements)

References 32 publications

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“…Brassica crops (UN, ), particularly oilseed rape (OSR; Brassica napus L., 2n=38, AACC), cover many acres worldwide and are important sources of edible oil and biodiesel (Döring et al ., ; Xia et al ., ). In China, OSR is also considered an attractive ornamental crop, and several areas are well known for their OSR flower tourism, including Luoping County in Yunnan Province, Wuyuan County in Jiangxi Province, Jingmen County in Hubei Province, Menyuan County in Qinhai Province and Anshun County in Guizhou Province (Fu et al ., ). OSR flower tourism has become an important part of the local economy.…”

Section: Introductionmentioning

confidence: 97%

Production of red‐flowered oilseed rape via the ectopic expression of Orychophragmus violaceus OvPAP2

Chen

Pan

et al. 2017

Plant Biotechnology Journal

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SummaryOilseed rape (Brassica napus L.), which has yellow flowers, is both an important oil crop and a traditional tourism resource in China, whereas the Orychophragmus violaceus, which has purple flowers, likely possesses a candidate gene or genes to alter the flower colour of oilseed rape. A previously established B. napus line has a particular pair of O. violaceus chromosomes (M4) and exhibits slightly red petals. In this study, the transcriptomic analysis of M4, B. napus (H3), and O. violaceus with purple petals (OvP) and with white petals (OvW) revealed that most anthocyanin biosynthesis genes were up‐regulated in both M4 and OvP. Read assembly and sequence alignment identified a homolog of AtPAP2 in M4, which produced the O. violaceus transcript (OvPAP2). The overexpression of OvPAP2 via the CaMV35S promoter in Arabidopsis thaliana led to different levels of anthocyanin accumulation in most organs, including the petals. However, the B. napus overexpression plants showed anthocyanin accumulation primarily in the anthers, but not the petals. However, when OvPAP2 was driven by the petal‐specific promoter XY355, the transgenic B. napus plants produced red anthers and red petals. The results of metabolomic experiments showed that specific anthocyanins accumulated to high levels in the red petals. This study illustrates the feasibility of producing red‐flowered oilseed rape, thereby enhancing its ornamental value, via the ectopic expression of the OvPAP2 gene. Moreover, the practical application of this study for insect pest management in the crop is discussed.

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Section: Introductionmentioning

confidence: 97%

Production of red‐flowered oilseed rape via the ectopic expression of Orychophragmus violaceus OvPAP2

Chen

Pan

et al. 2017

Plant Biotechnology Journal

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“…Xie et al studied G. hirsutum (genomic AADD) and found that cotton has an increased miRNA relative to its two diploid ancestors (Xie and Zhang 2015). In addition, in the Brassica, Fu et al found that the number and expression levels of miRNAs in the newly synthesized B. napus from different generations increased compared with the parents (Fu et al 2016a). These conclusions are consistent with our findings, indicating that the process of hybridization and doubling of the multiploidization results in a general increase in miRNAs.…”

Section: Flowering Trait Analysismentioning

confidence: 99%

Peer Review #3 of "Analysis of small RNA changes in different Brassica napus synthetic allopolyploids (v0.1)"

Markandan¹

2019

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Allopolyploidy is an evolutionary and mechanisticaly intriguing process involving the reconciliation of two or more sets of diverged genomes and regulatory interactions, resulting in new phenotypes. In this study, we explored the small RNA changes of eight F2 synthetic B. napus using small RNA sequencing. We found that a part of miRNAs and siRNAs were non-additively expressed in the synthesized B. napus allotetraploid. Differentially expressed miRNAs and siRNAs differed among eight F2 individuals, and the differential expression of miR159 and miR172 was consistent with that of flowering time trait. The GO enrichment analysis of differential expression miRNA target genes found that most of them were concentrated in ATP-related pathways, which might be a potential regulatory process contributing to heterosis. In addition, the number of siRNAs present in the offspring was significantly higher than that of the parent, and the number of high parents was significantly higher than the number of low parents. The results have shown that the differential expression of miRNA lays the foundation for explaining the trait separation phenomenon, and the significant increase of siRNA alleviates the shock of the newly synthesized allopolyploidy. It provides a new perspective between small RNA changes and trait separation in the early stages of allopolyploid polyploid formation.

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“…Rapeseed (Brassica napus L.), an ideal plant for phytoremediation, is an important source of edible vegetable oil, vegetable, animal fodder, green manure and biodiesel [11]. Breeding rapeseed cultivars with Pb-tolerant require germplasms and genetic loci related to Pb tolerance.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide association study (GWAS) reveals genetic loci of lead (Pb) tolerance during seedling establishment in rapeseed (Brassica napus L.)

Zhang

Xiao

et al. 2020

BMC Genomics

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Background: Lead (Pb) pollution in soil has become one of the major environmental threats to plant growth and human health. Safe utilization of Pb contaminated soil by phytoremediation require Pb-tolerant rapeseed (Brassica napus L.) accessions. However, breeding of new B. napus cultivars tolerance to Pb stress has been restricted by limited knowledge on molecular mechanisms involved in Pb tolerance. This work was carried out to identify genetic loci related to Pb tolerance during seedling establishment in rapeseed. Results: Pb tolerance, which was assessed by quantifying radicle length (RL) under 0 or 100 mg/L Pb stress condition, shown an extensive variation in 472 worldwide-collected rapeseed accessions. Based on the criterion of relative RL > 80%, six Pb-tolerant genotypes were selected. Four quantitative trait loci (QTLs) associated with Pb tolerance were identified by Genome-wide association study. The expression level of nine promising candidate genes, including GSTUs, BCATs, UBP13, TBR and HIPP01, located in these four QTL regions, were significantly higher or induced by Pb in Pb-tolerant accessions in comparison to Pb-sensitive accessions.Conclusion: To our knowledge, this is the first study on Pb-tolerant germplasms and genomic loci in B. napus. The findings can provide valuable genetic resources for the breeding of Pb-tolerant B. napus cultivars and understanding of Pb tolerance mechanism in Brassica species.

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Research progress and strategies for multifunctional rapeseed: A case study of China

Cited by 77 publications

References 32 publications

Production of red‐flowered oilseed rape via the ectopic expression of Orychophragmus violaceus OvPAP2

Production of red‐flowered oilseed rape via the ectopic expression of Orychophragmus violaceus OvPAP2

Peer Review #3 of "Analysis of small RNA changes in different Brassica napus synthetic allopolyploids (v0.1)"

Genome-wide association study (GWAS) reveals genetic loci of lead (Pb) tolerance during seedling establishment in rapeseed (Brassica napus L.)

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