Research on the Structure of Fish Collagen Nanofibers Influenced Cell Growth

Zhou, Gang; Zhang, Guimin; Wu, Zhe; Hou, Yongzhao; Yan, M.; Liu, Haifeng; Niu, Xufeng; Ruhan, A; Fan, Yubo

doi:10.1155/2013/764239

Cited by 7 publications

(6 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, they also found that higher collagen concentration (at least 20%) was needed when acetic acid was used as the solvent to prepare the spinning solution. Fish collagen electrospinning also has been reported by other groups and the produced nanofibers were found to support cell biological activities [76][77][78].…”

Section: Effects Of Collagen Sources On Electrospun Collagen Nanofiberssupporting

confidence: 61%

Electrospun Collagen Nanofibers and Their Applications in Skin Tissue Engineering

Law

Liau

Saim

et al. 2017

Tissue Eng Regen Med

168

107

View full text Add to dashboard Cite

Electrospinning is a simple and versatile technique to fabricate continuous fibers with diameter ranging from micrometers to a few nanometers. To date, the number of polymers that have been electrospun has exceeded 200. In recent years, electrospinning has become one of the most popular scaffold fabrication techniques to prepare nanofiber mesh for tissue engineering applications. Collagen, the most abundant extracellular matrix protein in the human body, has been electrospun to fabricate biomimetic scaffolds that imitate the architecture of native human tissues. As collagen nanofibers are mechanically weak in nature, it is commonly cross-linked or blended with synthetic polymers to improve the mechanical strength without compromising the biological activity. Electrospun collagen nanofiber mesh has high surface area to volume ratio, tunable diameter and porosity, and excellent biological activity to regulate cell function and tissue formation. Due to these advantages, collagen nanofibers have been tested for the regeneration of a myriad of tissues and organs. In this review, we gave an overview of electrospinning, encompassing the history, the instrument settings, the spinning process and the parameters that affect fiber formation, with emphasis given to collagen nanofibers' fabrication and application, especially the use of collagen nanofibers in skin tissue engineering.

show abstract

Section: Effects Of Collagen Sources On Electrospun Collagen Nanofiberssupporting

confidence: 61%

Electrospun Collagen Nanofibers and Their Applications in Skin Tissue Engineering

Law

Liau

Saim

et al. 2017

Tissue Eng Regen Med

168

107

View full text Add to dashboard Cite

show abstract

“…Its pore size was about 100-300 m, and its porosity was about 80%. Cellular activities were affected by chemical composition, conformation, porosity, and hydrophobicity of matrix [25]. The osteogenesis ability of nHAC/PLA composites were confirmed by osteoblasts culture and animal model tests [9].…”

Section: Discussionmentioning

confidence: 92%

Vascularization of Nanohydroxyapatite/Collagen/Poly(L-lactic acid) Composites by Implanting Intramuscularly In Vivo

Wang

Chang

Qiu

et al. 2014

International Journal of Polymer Science

View full text Add to dashboard Cite

It still remains a major challenge to repair large bone defects in the orthopaedic surgery. In previous studies, a nanohydroxyapatite/collagen/poly(L-lactic acid) (nHAC/PLA) composite, similar to natural bone in both composition and structure, has been prepared. It could repair small sized bone defects, but they were restricted to repair a large defect due to the lack of oxygen and nutrition supply for cell survival without vascularization. The aim of the present study was to investigate whether nHAC/PLA composites could be vascularized in vivo. Composites were implanted intramuscularly in the groins of rabbits for 2, 6, or 10 weeks (n=5×3). After removing, the macroscopic results showed that there were lots of rich blood supply tissues embracing the composites, and the volumes of tissue were increasing as time goes on. In microscopic views, blood vessels and vascular sprouts could be observed, and microvessel density (MVD) of the composites trended to increase over time. It suggested that nHAC/PLA composites could be well vascularized by implanting in vivo. In the future, it would be possible to generate vascular pedicle bone substitutes with nHAC/PLA composites for grafting.

show abstract

“…4,[7][8][9] Scientists from Nanyang Technological University (NTU) in Singapore have demonstrated that collagen derived from fish scales is highly effective across a variety of biomedical applications such as wound healing. 10 Research conducted by Zhou et al (2013), strongly suggests that fish scales containing collagen can increase blood vessel and lymph formation, thereby promoting tissue repair and regeneration. 11 However, the findings of these studies suggest that the viability of the scales and thorns of Gurami and Kuniran fish remains unproven.…”

Section: Introductionmentioning

confidence: 99%

“…10 Research conducted by Zhou et al (2013), strongly suggests that fish scales containing collagen can increase blood vessel and lymph formation, thereby promoting tissue repair and regeneration. 11 However, the findings of these studies suggest that the viability of the scales and thorns of Gurami and Kuniran fish remains unproven. It is known that Gurami (fresh water) and Kuniran (salt water) are the types of fish most frequently consumed by the population of Jember, East Java, Indonesia.…”

Section: Introductionmentioning

confidence: 99%

The role of Kuniran (U. moluccensis) and Gurami (O. goramy) fish thorns and scales in increasing salivary leukocyte and monocyte cells viability against Streptococcus mutans

Dewanti

Susilawati

et al. 2019

Dent. J.

View full text Add to dashboard Cite

Background: Kuniran thorns and Gurami fish scales are rich in protein and minerals such as dentin believed to increase cell viability against Streptococcus mutans (S. mutans) that causes dental caries. These, in turn, can cause systemic diseases if left untreated. Purpose: This study aims to analyze the influence of Kuniran thorns and Gurami fishes scales on the viability of monocytes and salivary leukocytes against S. mutans. Methods: Monocytes and leukocytes salivary cells were placed on a microtiter plate and treated according to the nature of each group. This study comprised the following groups: control group: untreated; S. mutans group: induced by S. Mutans; Gurami thorn group: thorns + S. Mutans; Gurami scales group: scales + S. Mutans; Kuniran thorn group: thorns + S. Mutans; Kuniran scales group: scales + S. mutans. Viability analysis involved staining with Tripan Blue. Furthermore, the number of viable cells (white) was calculated under an inverted microscope at 200 times magnification from five fields of view. Data was analyzed by means of an ANOVA test followed by LSD test. Results: The ANOVA and LSD tests confirmed significant differences (0.01<P). Kuniran thorns and Gurami fish scales increased the viability of monocytes and salivary leukocytes, but not significantly. The content of flavonoids, amino acids, omega 3, omega 6 and antioxidants increased leukocyte metabolism, thereby increasing cell resistance to S. mutans infection. Conclusion: Kuniran thorns (U. moluccensis) and Gurami (O. goramy) fish scales increase the viability of salivary leukocyte and monocyte cells against Streptococcus mutans.

show abstract

Research on the Structure of Fish Collagen Nanofibers Influenced Cell Growth

Cited by 7 publications

References 32 publications

Electrospun Collagen Nanofibers and Their Applications in Skin Tissue Engineering

Electrospun Collagen Nanofibers and Their Applications in Skin Tissue Engineering

Vascularization of Nanohydroxyapatite/Collagen/Poly(L-lactic acid) Composites by Implanting Intramuscularly In Vivo

The role of Kuniran (U. moluccensis) and Gurami (O. goramy) fish thorns and scales in increasing salivary leukocyte and monocyte cells viability against Streptococcus mutans

Contact Info

Product

Resources

About