Research on the effect and underlying molecular mechanism of Cangzhu in the treatment of gouty arthritis

Li, Chao; Wang, Chan; Yijing, Guo; Wen, Rou; Yan, Liping; Zhang, FengRong; Gong, Qianfeng; Yu, Huan

doi:10.1016/j.ejphar.2022.175044

Cited by 18 publications

(11 citation statements)

References 48 publications

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“…Evidence has demonstrated the crosstalk between atractylenolide I-induced downregulation of IL1B, IL6, and TNF and TLR4 signaling [81]. Noteworthily, atractylenolide I has been determined as one of the main active components of Rhizoma Atractylodis to treat gouty arthritis via downregulating IL1B, IL6, TNF, and uric acid [82]. Consistent with the previous studies, our data imply that Simiao pill may treat HUA by targeting the release of inflammatory factors and inflammation-related signaling pathways.…”

Section: Discussionsupporting

confidence: 89%

[Retracted] A Network Pharmacology Method Combined with Molecular Docking Verification to Explore the Therapeutic Mechanisms Underlying Simiao Pill Herbal Medicine against Hyperuricemia

Yin

et al. 2023

BioMed Research International

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Objective. Hyperuricemia (HUA) is a common metabolic disease caused by disordered purine metabolism. We aim to reveal the mechanisms underlying the anti-HUA function of Simiao pill and provide therapeutic targets. Methods. Simiao pill-related targets were obtained using Herbal Ingredients’ Targets (HIT), Traditional Chinese Medicine Systems Pharmacology (TCMSP), and Traditional Chinese Medicine Integrated Database (TCMID). HUA-associated targets were retrieved from GeneCards, DisGeNET, and Therapeutic Targets Database (TTD). Protein-protein interaction (PPI) network was constructed using the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database, ggraph and igraph R packages. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed using ClusterProfiler. The top 10 core targets were identified through cytoHubba. Molecular docking was conducted using PyMOL and AutoDock high-performance liquid chromatograph (HPLC) analysis was performed to identify effective compounds of Simiao pill. Results. Simiao pill-HUA target network contained 80 targets. The key targets were mainly involved in inflammatory responses. Insulin (INS), tumor necrosis factor (TNF), interleukin-6 (IL6), interleukin 1 beta (IL1B), vascular endothelial growth factor A (VEGFA), leptin (LEP), signal transducer and activator of transcription 3 (STAT3), C-C motif chemokine ligand 2 (CCL2), interleukin-10 (IL10), and toll like receptor 4 (TLR4) were the top 10 targets in the PPI network. GO analysis demonstrated the main implication of the targets in molecular responses, production, and metabolism. KEGG analysis revealed that Simiao pill might mitigate HUA through advanced glycation end-product- (AGE-) receptor for AGE- (RAGE-) and hypoxia-inducible factor-1- (HIF-1-) associated pathways. IL1B, IL6, IL10, TLR4, and TNF were finally determined as the promising targets of Simiao pill treating HUA. Through molecular docking and HPLC analysis, luteolin, quercetin, rutaecarpine, baicalin, and atractylenolide I were the main active compounds. Conclusions. Simiao pill can mitigate HUA by restraining inflammation, mediating AGE-RAGE- and HIF-1-related pathways, and targeting IL1B, IL6, IL10, TLR4, and TNF.

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Section: Discussionsupporting

confidence: 89%

[Retracted] A Network Pharmacology Method Combined with Molecular Docking Verification to Explore the Therapeutic Mechanisms Underlying Simiao Pill Herbal Medicine against Hyperuricemia

Yin

et al. 2023

BioMed Research International

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“…The cut‐off values of some parameters were as follows: species, Homo sapiens; minimum interaction, high confidence (0.9); data type, TSV format. Subsequently, Cytoscape 3.8.2 (Li et al, 2022) software was used to visualize the PPI network. CytoHubba, a plug‐in for Cytoscape was used to screen the top 10 core genes with higher degree.…”

Section: Methodsmentioning

confidence: 99%

Coptidis rhizoma and evodiae fructus against lipid droplet deposition in nonalcoholic fatty liver disease‐related liver cancer by AKT

Hao

Deng

et al. 2023

Chem Biol Drug Des

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Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease in the world. NAFLD has become one of the major factors contributing to hepatocellular carcinoma (HCC) development. However, there are no clear targets and therapeutic drugs for NAFLD‐related liver cancer. This study explored the active compounds, target and mechanism of coptidis rhizoma and evodiae fructus in the treatment of NAFLD‐related liver cancer based on the network pharmacology and experimental verification. There were 455 intersection targets of NAFLD‐related liver cancer, and 65 drug‐disease common targets. AKT1 has the highest degree, indicating that it may be a key target of coptidis rhizoma and evodiae fructus in the treatment of NAFLD‐related liver cancer. The expression level of AKT1 was high in high‐risk group, and the overall survival rate was lower than that in low‐risk group. After oleic acid induction, p‐AKT expression and lipid droplet deposition were promoted in HepG2 cells. Quercetin and resveratrol reduced lipid droplet deposition in vivo. Moreover, quercetin inhibited p‐AKT expression, resveratrol both reduced the expression of p‐AKT and AKT. The overall findings suggested that quercetin inhibited AKT in the treatment of NAFLD‐related liver cancer.

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“…Li et al identified the effective components and the mechanism of atractylodis rhizome extract in the treatment of gouty arthritis through network pharmacology and verified their results through experiments. Atractylodes macrocephala could lower the levels of TGF-β1, PGE2, UA, IL-1β, IL-6, and TNF-α and XOD activity and can regulate apoptosis-related pathways, which may be the mechanism of atractylodes macrocephala for gout treatment [44]. The atractylodes macrocephala extract can inhibit XOD activity, reduce uric acid level, and decrease inflammation in gouty arthritis rats by hindering the formation of inflammatory factors such as IL-1β and TNF-α [45].…”

Section: Herbal Medicines Originated From Asteraceaementioning

confidence: 99%

Effects and Mechanisms of Traditional Chinese Medicine and Related Active Constituents Treating Gout

2023

Curr Res Cmpl Alt Med

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Gout is an inherited metabolic disorder resulting from abnormalities in purine metabolism manifesting as episodes of acute inflammatory arthritis. The incidence of gout shows an increasing tendency each year, due to the complicated pathogenesis, the currently available drugs have poor treatment effects on it. Therefore, the development of effective drugs for gout treatment is urgently needed. Because of its high biological activity and safety, Traditional Chinese medicine (TCM) has significant advantages in the prevention and treatment of gout. This paper systematically reviewed the related research on TCM's treatment of gout in the previous five years, and summarized the actions and mechanisms of 25 Chinese herbal extracts, 38 Chinese medicinal monomers, and 19 Chinese medicinal compounds in the treatment of gout, including serum uric acid levels reduction, anti-inflammation, and anti-oxidative stress. These primarily involve the signaling pathways NLRP3/ASC/caspase, TLRs/MYD88/NF-κB, ATP/ P2X7R, and others. Furthermore, the limitations and shortcomings of the experimental study on the treatment of gout with TCM are discussed and appropriate measures are proposed. To summarize, TCM is likely to be a potential candidate for gout treatment, but more clinical research is needed to fully understand its role in the treatment of gout.

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Research on the effect and underlying molecular mechanism of Cangzhu in the treatment of gouty arthritis

Cited by 18 publications

References 48 publications

[Retracted] A Network Pharmacology Method Combined with Molecular Docking Verification to Explore the Therapeutic Mechanisms Underlying Simiao Pill Herbal Medicine against Hyperuricemia

[Retracted] A Network Pharmacology Method Combined with Molecular Docking Verification to Explore the Therapeutic Mechanisms Underlying Simiao Pill Herbal Medicine against Hyperuricemia

Coptidis rhizoma and evodiae fructus against lipid droplet deposition in nonalcoholic fatty liver disease‐related liver cancer by AKT

Effects and Mechanisms of Traditional Chinese Medicine and Related Active Constituents Treating Gout

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