Research on the early stages of spore germination in Bacillus licheniformis using dynamic phase microscopy

Tychinskii, V. P.; Nikolaev, Yu. A.; Lisovskii, V. V.; Kretushev, A. V.; Vyshenskaya, T. V.; Mulyukin, A. L.; Suzina, N. E.; Дуда, В. И.; Эль-Регистан, Г. И.

doi:10.1134/s0026261707020063

Cited by 5 publications

(4 citation statements)

References 11 publications

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“…are commonly present in soil microbiota. These species can withstand harsh conditions, including limited nutrients, because of their endospore forming ability (Tychinskii et al 2007;Soni et al 2016). The spores are very tolerant to heat, cold, radiation, and alkalinity.…”

Section: Introductionmentioning

confidence: 99%

Characterization of moderately thermostable α-amylase-producing Bacillus licheniformis from decaying potatoes and sweet potatoes

Ashraf

Arshad

Rahman

et al. 2018

BioRes

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Bacillus licheniformis is an endospore-forming bacterium that is commonly present in soil. The aim of the present study was to isolate and characterize local strains of α-amylase producing B. licheniformis. Soil samples were collected from the decaying surfaces of potatoes and sweet potatoes. The samples were identified by Gram staining, spore staining, and motility testing under aerobic conditions. Twenty-three isolates were found to be from the Bacillus genus and six of those (26%) were found to be B. licheniformis. Two representative samples were run on API 20E and API 50 CH biochemical kits, and 16S rDNA polymerase chain reaction. The isolates were confirmed to be B. licheniformis by the API-web program and molecular detection. Partially purified α-amylase was characterized to determine the effect of the incubation period, temperature tolerance, and pH stability. The activity peaked at 740 mU/mL after 42 h of culturing. The relative activity reached a maximum at 55 °C and a pH of 8.0. The decaying surfaces of potatoes and sweet potatoes are promising sources of α-amylase-producing strains of B. licheniformis that can tolerate both a high temperature and drastic pH level.

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Section: Introductionmentioning

confidence: 99%

Characterization of moderately thermostable α-amylase-producing Bacillus licheniformis from decaying potatoes and sweet potatoes

Ashraf

Arshad

Rahman

et al. 2018

BioRes

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“…Определенную зависимость фазовой толщины от этапа жизненного цикла выявила группа ученых при исследовании стадий прорастания спор. Споры, прорастая, растворяют наружную оболочку и принимают «распластанную форму» [10]. Фибробласты, напротив, готовясь к митозу, подбирают отростки и приобретают округлую форму.…”

Section: результаты и обсуждениеunclassified

“…Такой подход позволяет осуществлять неинвазивные исследования клеточной структуры, получать изображение с нанометровым разрешением и проводить анализ оптических свойств объекта [3,6], максимально исключая погрешности и присутствие артефактов. Модуляционная интерференционная микроскопия уже используется в изучении таких объектов, как клетки крови, опухолевые клетки, нейрон-глиальная сеть, а также бактерии и споры микроорганизмов [7][8][9][10][11].…”

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The use of the interference microscopy to study structural characteristics of cultured dermal fibroblasts

Nefedova

Rossinskaya

2018

Alʹm. klin. med.

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Rationale: The use of electron, nuclear power and confocal microscopy for the screening of biologically active compounds, medical products and express diagnostics of some diseases at the cell level is associated with laborand time-consuming sample preparation, which cannot exclude potential measurement errors and artifacts. The modulation interference microscopy does not have these disadvantages; it allows for non-invasive studies of cell structures, imaging with nanometer resolution and analysis of the optical properties of an object.Aim: To assess the potential of the interference microscopy in the evaluation of morphofunctional characteristics of in vitro mitomycin conditioned cultured cell nuclei (dermal fbroblasts taken as a model).Materials and methods: Native culture of human dermal fbroblasts of the 6th passage, grown on glass with mirror coating in the cell culture laboratory of the Institute of Experimental Medicine and Biotechnology of Samara State Medical University (Russia), was examined with a modulation interference microscope MIM-340 (JSC PA UOMZ, Russia). Changes over time in the structural characteristics of dermal fbroblast nuclei conditioned with mitomycin were evaluated. The control group included fbroblasts cultured in the same conditions on glass with mirror coating without mitomycin. Imaging with MIM-340 was done at three hours, one and four days after adding the cytostatic. The control group was assessed at the same time points.Results: We have shown that the cell culture grown on dielectric glasses does not diﬀer in its morphofunctional characteristics from the culture grown on culture plastics. This proves the possibility to study the adhesive native culture using interference microscopy. We have found that the cells respond to a single mitomycin 0.04% exposure with a change to a globular shape and a sharp increase in the nuclear phase thickness (217.8 vs. 142.18 nm in the control group, p ≤ 0.05). Thereafter, the morphofunctional characteristics of the cells are restored, which is confrmed by the changes over time in the culture density, cell shape and size, and the phase thickness of the nucleus.Conclusion: The results obtained make it possible to recommend the method of modulation interference microscopy for evaluation of toxicity and biocompatibility of drugs, medical products and physical factors for diagnosis and treatment.

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“…Popescu et al, 2005;Popescu et al, 2006;Rappaz et al, 2007;Rappaz et al, 2008;Rappaz et al, 2009;Yusipovich, I et al, 2009;Yusipovich et al, 2011), fibroblasts (Dunn & Zicha, 1995), lymphocytes (Tychinsky et al, 2012), neurons (Rappaz et al, 2005;Yusipovich et al, 2006), nerve fibers (see e.g. Bibineyshvili et al, 2013;Brazhe et al, 2008;LaPorta & Kleinfeld, 2012;Maksimov et al, 2001), mast cells (Bondarenko et al, 2013), any microbiological objects (Tychinskii et al, 2007;Yusipovich et al, 2012;Zhurina et al, 2013) and others. In these work we propose a new assay for estimation a number of non-activated, activated and dead isolated peritoneal macrophages in a sample in vitro based on cell phase images.…”

Section: Introductionmentioning

confidence: 99%

Application of Phase Images for Estimation of Peritoneal Macrophages State

Yusipovich¹,

Baizhumanov²,

Казакова³

et al. 2015

IJB

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Procedure based on phase images obtained by the method of Laser Interference Microscopy (LIM) for detection of number non-activated, activated and dead isolated peritoneal macrophages in a sample in vitro was provided. The phase images provide quantitative estimates of the optical path difference (OPD) in each point of object. The OPD varies with cell thickness and concentration of cellular substances. Therefore, it provides useful information on cell structure, localization of subcellular structures, local concentrations of substances and dry mass of cell. In these work we propose a new assay for estimation a number of non-activated, activated and dead isolated peritoneal macrophages in a sample in vitro based on cell phase images. The results were compared with phagocytic index, which was evaluated using latex beads, and the percentage of cells with damaged membranes (dead cells). It was shown that with increasing time after the isolation, the percentage of dead macrophages estimated by LIM was similar to that of the cells with damaged membranes. However, the percentage of activated cells calculated using latex beads exceeded the percentage calculated using the phase images significantly.

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Research on the early stages of spore germination in Bacillus licheniformis using dynamic phase microscopy

Cited by 5 publications

References 11 publications

Characterization of moderately thermostable α-amylase-producing Bacillus licheniformis from decaying potatoes and sweet potatoes

Characterization of moderately thermostable α-amylase-producing Bacillus licheniformis from decaying potatoes and sweet potatoes

The use of the interference microscopy to study structural characteristics of cultured dermal fibroblasts

Application of Phase Images for Estimation of Peritoneal Macrophages State

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