Mesoporous silica nanoparticles (MSNs),
the inorganic nanomaterials
with stable physicochemical property and versatile surface functional
groups, have been increasingly studied and applied in biomedical field
as nanocarriers. There are a limited number of reports on their potential
applications in clinical dentistry. Herein, we fabricated the fluorescence-labeled
MSNs (RITC-NPs) and investigated for the first time their infiltration
profiles in the dentinal tubules of extracted permanent teeth prepared
by different irrigation approaches. Ethylenediaminetetraacetic acid
(EDTA) enabled removal of the smear layer efficiently and exposure
of the dentinal tubules on the root canal walls. Confocal scanning
laser microscopy showed that the RITC-NPs could accumulate on the
root canal walls, and effectively infiltrate into the dentin. In general,
the fluorescence intensity in dentin from EDTA-treated group was much
higher than that from the ultrasound-treated group, and stronger red
fluorescence was observed at the crown and middle sections than the
apical one in the EDTA group. The field-emission gun scanning electron
microscopy further confirmed the infiltration effects of RITC-NPs
in the dentin, through the patent dentinal tubules. The present findings
on the infiltration of MSNs in the root canal system may provide a
potential approach for further applying MSN-encapsulated antimicrobials
in advanced endodontic therapy.