ADS40 is the first commercial three line scanner(TLS) Airborne Digital Sensor developed by LH Systems jointly with DLR and launched in 2000.The unique hardware design and dataset processing workflow of ADS40 bring challenges to traditional calibration theory and methods. Considering the imagery acquirement and interior orientation methods of ADS40, the ADS40 test field calibration using posteriori compensation method is proposed: An analysis is made to the systematic error of ADS40’s cam file. A set of first order bivariate polynomial functions is developed to fit the systematic error, and the solution method is deduced. With the practical ADS40 dataset, a set of experiments is done, and the experimental results demonstrate the effectiveness and feasibility of proposed method.