Research on Creation of Methods for Determining Betulin in Birch Bark Processing Products Using HPLC

Abstract

The paper assesses the possibility of creating a method for determining betulin in birch bark processing products using HPLC. The relevance and feasibility of developing an analysis are associated with an increase in the production of various products from birch bark and the need for a reliable and rapid way to assess their quality. The basis of the hypothesis is the reproducibility of the determination results of betulin in birch bark processing products by means of chromatography-mass spectrometry using the method of reversed-phase high-performance liquid chromatography. Initial qualitative and quantitative analyzes of betulin-containing extracts are carried out using gas chromatography-mass spectrometry methods. The reproducibility of the results obtained using HPLC has been verified. HPLC analysis of betulin-containing extracts and agrochemical “Stimulin” have revealed a correlation of the quantitative composition of betulin-containing preparations.