Research of photon correlation technology

Zou, Lianying; Gao, Ning; Xiao, Qian

doi:10.1088/1757-899x/711/1/012097

Cited by 2 publications

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The correlation in Eq. 1 can be calculated with a computerbased software or hardware correlator [20][21][22][23]. With the software correlator, photon counts and arrival times are imported into the computer and then correlated with relevant programs.…”

Section: Discussionmentioning

confidence: 99%

A Comprehensive Review of Fluorescence Correlation Spectroscopy

Lei

et al. 2021

Front. Phys.

View full text Add to dashboard Cite

Fluorescence correlation spectroscopy (FCS) is a powerful technique for quantification of molecular dynamics, and it has been widely applied in diverse fields, e.g., biomedicine, biophysics, and chemistry. By time-correlation of the fluorescence fluctuations induced by molecules diffusing through a focused light, FCS can quantitatively evaluate the concentration, diffusion coefficient, and interaction of the molecules in vitro or in vivo. In this review, the basic principle and implementation of FCS are introduced. Then, the advances of FCS variants are reviewed, covering dual-color FCCS, multi-focus FCS, pair correlation function (pCF), scanning FCS, focus-reduced FCS, SPIM-FCS, and inverse-FCS. Besides, the applications of FCS are demonstrated with the measurement of local concentration, hydrodynamic radius, diffusion coefficient, and the interaction of different molecules. Lastly, a discussion is given by summarizing the pros and cons of different FCS techniques, as well as the outlooks and perspectives of FCS.

show abstract

Section: Discussionmentioning

confidence: 99%

A Comprehensive Review of Fluorescence Correlation Spectroscopy

Lei

et al. 2021

Front. Phys.

View full text Add to dashboard Cite

show abstract

Binning method for artifact-free time-tag based correlation function calculations

Urquidi,

Brazard,

Adachi

2024

Opt. Lett.

View full text Add to dashboard Cite

Correlation functions are nowadays routinely computed using time-tagged photon information instead of a hardware autocorrelator. The algorithm developed by Laurence et al. [Opt. Lett. 31, 829 (2006)10.1364/OL.31.000829] is a powerful example. Despite its ease of implementation and fast computation process, it presents a prevalent noisy feature at the short time-lag range when computed on commonly used logarithmically spaced bins. We identified that arbitral logarithmic spacing produces the mismatch between the edges of generated bins and acquisition frequency, resulting in an aliasing artifact at the short time-lag range of the correlation function. We introduce a binning method that considers the acquisition frequency during the bin generation. It effectively eliminates the artifact and improves the accuracy of the autocorrelation. Applying the binning method herein can be particularly crucial when one extracts photophysical processes from fluorescence correlation spectroscopy or the diffusion coefficient of nanoparticles from dynamic light scattering at the time range below 10−5 s lag time.

show abstract

Research of photon correlation technology

Cited by 2 publications

References 8 publications

A Comprehensive Review of Fluorescence Correlation Spectroscopy

A Comprehensive Review of Fluorescence Correlation Spectroscopy

Binning method for artifact-free time-tag based correlation function calculations

Contact Info

Product

Resources

About